Reproductive Engineering Home
Go to: IVF and Fertility Dashboard

KYUDO/CARD
IVF PRODUCTS

Reagents FOR MOUSE

Fertiup CP - Cryopreservation Media

Fertiup PM
 -  Preincubation Media

CARD Media
 - for IVF procedure

Card HyperOva - Superovulation Reagent

CARD IVF Companion Reagents -  mHTF; KSOM; DMSO; DAP213; Sucrose

Hardware Accessories - Sperm Straws, Freezing Cassettes and Canisters and more!

CARD Cold Transport Kit - for safe transport of cauda epididymis and embryos


Reagents FOR RAT

CARD FERTIUP® Rat Sperm Cryopreservation Agent
- high performance reagent for rat sperm cryopreservation

CARD Rat Sperm Freezing Kit - sperm straw holder and float to promote optimal sperm freezing

CARD Rat IVF Medium
- high performance reagent for rat IVF


FLYERS and BROCHURES

Kyudo/CARD Products for RAT IVF Flyer
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CARD FERTIUP® Rat Sperm Cryopreservation Agent flyer
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Product Information

PROTOCOL: Rat Sperm Cryopreservation
RAT Products FAQ

Ordering Information

CARD Technical Manual: "Reproductive Engineering Techniques in Mice" by Naomi Nakagata (3rd edition)

Instruction Manuals
CARD FERTIUP® Cryoprotectant
CARD FERTIUP®  Preincubation Media
CARD MEDIUM®
CARD FERTIUP® : PM - CARD MEDIUM® Set

VIDEOS
Protocols:
Rat sperm cryopreservation protocol
Frozen rat sperm thawing protocol

Center for Animal Resources and Development (CARD), Kumamoto University
URL: https://nakagatarbi.com


2023 KYUDO
Product Catalog


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Download (English)
Download (Japanese)
Download (Chinese)


LEARN

CARD Technical Manual: "Reproductive Engineering Techniques in Mice" by Naomi Nakagata (3rd edition)

Journal Article: Nakagata et. al. (2020), Establishment of sperm cryopreservation and in vitro fertilization protocols for rats


Cosmo Bio Brand IVF Products

for MOUSE- HTF; KSOM; mWM; Sucrose; DMSO; DAP213; R18S3 

For RAT: - HTF; mR1ECM; Rat KSOM; SPB1; P10 PEPeS  

Defined reagents for PIG:  Kits; POE-CM; POM; HP-POM; dbcAMP; SEM; PFM; PZM-5; PBM


4BioDx Brand Products

AKAP4 ELISA Kits and Abs - Test fresh or frozen semen to determine sperm quality and fertility potential. 

IVF and Fertility Dashboard

Reproductive Engineering and IVF: Media, Solutions, and Accessories

A Treasure Box of Products for In Vitro Fertilization (IVF) Related Procedures

 Welcome to the IVF and Fertility dashboard at Cosmo Bio USA. Follow links in the navigation column at left to explore all of the more than one hundred IVF-related products available at Cosmo Bio USA from these fine suppliers.

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  KYUDO/CARD IVF PRODUCTS

 Cosmo Bio Brand IVF Products

4BioDx Brand Products

 A treasure box of reagents,
tools, and experience for
mouse and rat sperm cryopreservation and IVF

* Mouse and rat IVF accessory reagents.
* Porcine IVF reagents, media, and kits 

 ProAKAP4 (demonstrated
marker of 
sperm quality)
ELISA 
kits and antibodies. 10 species!

More About Our IVF Suppliers

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Kyudo Product Lineup

Product Name Size Catalog Number
CARD FERTIUP Cryoprotectant: CPA
500 ul KYD-001-05-EX
25 x 500 ul KYD-001-05-EX-X25
5 x 500 ul KYD-001-05-EX-X5
50 x 500 ul KYD-001-05-EX-X50
1 ml KYD-001-EX
25 x 1 ml KYD-001-EX-X25
5 x 1 ml KYD-001-EX-X5
50 x 1 ml KYD-001-EX-X50
CARD FERTIUP Preincubation Medium: PM
500 ul KYD-002-05-EX
25 x 500 ul KYD-002-05-EX-X25
5 x 500 ul KYD-002-05-EX-X5
50 x 500 ul KYD-002-05-EX-X50
1 ml KYD-002-EX
25 x 1 ml KYD-002-EX-X25
5 x 1 ml KYD-002-EX-X5
50 x 1 ml KYD-002-EX-X50
CARD MEDIUM

1 Kit KYD-003-EX
10 Kits KYD-003-EX-X10
5 Kits KYD-003-EX-X5
CARD FERTIUP PM 1 mL - CARD MEDIUM Set

1 Kit KYD-004-EX
10 Kits KYD-004-EX-X10
5 Kits KYD-004-EX-X5
CARD FERTIUP PM 0.5 mL - CARD MEDIUM Set

1 Kit KYD-005-EX
10 Kits KYD-005-EX-X10
5 Kits KYD-005-EX-X5
CARD Cold Transport Kit 1 Set KYD-006-EX
Cold storage solution for cauda epididymides

1 Kit KYD-007-EX
10 Kits KYD-007-EX-X10
5 Kits KYD-007-EX-X5
CARD mHTF (modified Human Tubal Fluid)
2 ml KYD-008-02-EX
25 x 2 ml KYD-008-02-EX-X25
5 x 2 ml KYD-008-02-EX-X5
50 x 2 ml KYD-008-02-EX-X50
5 ml KYD-008-05-EX
15 x 5 ml KYD-008-05-EX-X15
3 x 5 ml KYD-008-05-EX-X3
30 x 5 ml KYD-008-05-EX-X30
CARD HyperOva (Superovulation Reagent for mouse)
600 ul KYD-010-06-EX
25 x 600 ul KYD-010-06-EX-X25
5 x 600 ul KYD-010-06-EX-X5
50 x 600 ul KYD-010-06-EX-X50
1 ml KYD-010-EX
25 x 1 ml KYD-010-EX-X25
5 x 1 ml KYD-010-EX-X5
50 x 1 ml KYD-010-EX-X50
CARD KSOM
2 ml KYD-011-EX-2
5 ml KYD-011-EX-5
CARD 1M DMSO 1 ml KYD-012-EX
CARD DAP213
500 ul KYD-013-EX-0.5
1 ml KYD-013-EX-1
CARD 0.25M Sucrose
2 ml KYD-014-EX-2
5 ml KYD-014-EX-5
CARD HyperOva FD
1 x 2 ml KYD-015-EX
25 x 2 ml KYD-015-EX-X25
5 x 2 ml KYD-015-EX-X5
50 x 2 ml KYD-015-EX-X50
CARD Fertiup Rat Sperm Cryopreservation Agent
1.5 ml KYD-FR-001
10 x 1.5 ml KYD-FR-001-X10
5 x 1.5 ml KYD-FR-001-X5
CARD Rat IVF Medium
1 ml KYD-FR-002
10 x 1 ml KYD-FR-002-X10
5 x 1 ml KYD-FR-002-X5
CARD Rat Freezing Kit 1 Kit KYD-GAR-001
Reproductive Engineering Techniques in Mice Technical Manual 1 Piece KYD-MANUAL
Freezing Canister 1 Unit KYD-S018
Sperm Straws (10 Pieces x 10 Units) 10 Pieces KYD-S020X10
Triangular Cassette Short (10units) 10 Units KYD-S021
Straw Connector 1 Piece KYD-S025
Triangular Cassette Long (10units) 10 Units KYD-S035
Embryo manipulation instrument set 1 Set KYD-S036
Glass capillary 20PC 1 Set KYD-S037

Kyudo's products anchor Cosmo Bio USA's IVF and Fertility product category. In collaboration with the Center for Animal Resources and Development (CARD) at Kumamoto University, Kyudo products are recognized worldwide for quality and performance. Their innovative reagents for boosting the efficiencies of mouse sperm cryopreservation, superovulation, and IVF have contributed greatly to the worldwide increase in strain preservation via sperm cryopreservation.

What's NEW for IVF from KYUDO? (updated January 2021)

Superovulation reagent HyperOVA® is now also available in a freeze-dried format. HyperOva® FD delivers the same great performance but ships and stores at 4 C instead of dry ice and -80 C. 

CARD FERTIUP® Rat Sperm Cryopreservation Agent and CARD MEDIUM® for RAT IVF!


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Cosmo Bio offers our own brand of high performance IVF-related reagents with a focus on convenience and value. We offer an assortment of accessory reagents for mouse and rat IVF-related procedures in sizes that may be most compatible with your laboratory's work flow. Our reagent quality and consistency are assured. Cosmo Bio's IVF reagents are produced and packaged in Japan under ISO9001 global quality management standards. All reagents are sealed in glass ampoules and store at 4 degrees C with shelf lives of at least one year from the date of manufacture. 

For porcine IVF, Cosmo Bio offers a range of IVF reagents developed over more than 10 years by academic researchers and Japan's Institute for Functional Peptides with a focus on high performance animal-free, defined media for the production of high quality embryos.

What's NEW for IVF from Cosmo Bio? (updated January 2021) 

Nothing at the moment, Please check back later.

Cosmo Bio Product Lineup

Product Name Size Catalog Number
DAP213 (DMSO, Acetamide, Propylene Glycol) 10 x 1 ml CSR-R-T073
DMSO 1M 10 x 2 ml CSR-R-T072
HTF (Human Tubal Fluid)
10 x 2 ml CSR-R-B070
10 x 5 ml CSR-R-B071
M2
10 x 2 ml CSR-R-M083
10 x 5 ml CSR-R-M084
Modified Krebs-Ringer Bicarbonate (mKRB) medium 100 ml CSR-R-M193
Mouse KSOM (Potassium Simplex Optimized Medium)
10 x 2 ml CSR-R-B074
10 x 5 ml CSR-R-B075
mR1ECM (modified Rat 1-cell Embryo Culture Medium)
10 x 2 ml CSR-R-M191
10 x 5 ml CSR-R-M174
mWM (modified Whitten’s Medium)
10 x 2 ml CSR-R-B080
10 x 5 ml CSR-R-B081
P10 (Propylene Glycol in PB1) 10 x 2 ml CSR-R-P186
PB1
10 x 2 ml CSR-R-P185
10 x 5 ml CSR-R-P138
PEPeS (Percoll in PB1) 10 x 1 ml CSR-R-P187
R18S3 (Medium for Mouse Sperm Cryopreservation) 10 x 1 ml CSR-R-R082
Rat KSOM (Potassium Simplex Optimized Medium) 10 x 1 ml CSR-R-R148
SPB1
10 x 2 ml CSR-R-S187
10 x 5 ml CSR-R-S188
Sucrose 0.25M
10 x 2 ml CSR-R-Y077
10 x 5 ml CSR-R-Y078

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A major focus of 4BioDx (Lille, France) is the development and commercialization or reagents and ELISA kits to measure proAKAP4 concentration in semen. proAKAP4 is an indispensable molecule during spermatogenesis and any modification of proAKAP4 expression during spermatogenesis or modification in its metabolism will have consequences on sperm motility, hyper motility, sperm capacitation and then fertility. Consequently, measurement of proAKAP4 in sperm is an increasingly promising approach to evaluate semen quality prior to in vitro fertilization in both laboratory animals and commercial livestock.

What's NEW from 4BioDx? (updated January 2021):  

  • 4MID proAKAP4 ELISA Kits are available in ten species. Human. Pig. Horse. Bull. Dog. Camel. Ram. Rabbit. Goat. Mouse. 
  • 4BioDx has a new slide deck. "Functional Assay for Semen Quality and Boar Fertility Assessment". Please find it on this page.    

4BioDX Product Lineup

Catalog Number Product Name Size Clone No Applications Clonality Host Reactivity
BDX-4BDX-1501 Anti-Tau mAb [pS422] (clone 2H9) 100ug 2H9 IHC, IF, WB Monoclonal Mouse Rat, Mouse, Human
BDX-4BDX-1501S Anti-Tau mAb [pS422] (clone 2H9) 20ug 2H9 IHC, IF, WB Monoclonal Mouse Rat, Mouse, Human
BDX-4BDX-1502 Anti-Tau [pS199] pAb 80ul   IHC, IF, WB Polyclonal Rabbit Rat, Mouse, Human
BDX-4BDX-1502S Anti-Tau [pS199] pAb 40ul   IHC, IF, WB Polyclonal Rabbit Rat, Mouse, Human
BDX-4BDX-1503 Anti-APP [pT668] pAb 80ul   IHC, IF, WB Polyclonal Rabbit Rat, Mouse, Human
BDX-4BDX-1503S Anti-APP [pT668] pAb 40ul   IHC, IF, WB Polyclonal Rabbit Rat, Mouse, Human
BDX-4BDX-1602 Anti-AKAP4 mAb (clone 7E10) 100ug 7E10 IEM, ELISA, IF Monoclonal Mouse Sheep, Bovine, Equine, Porcine, Human
BDX-4BDX-1602S Anti-AKAP4 mAb (clone 7E10) 20ug 7E10 IEM, ELISA, IF Monoclonal Mouse Sheep, Bovine, Equine, Porcine, Human
BDX-4BDX-1701 Anti-pro AKAP4 mAb (clone 6F12) 100ug 6F12 IEM, ELISA, IF Monoclonal Mouse Sheep, Bovine, Equine, Porcine, Human
BDX-4BDX-1701S Anti-pro AKAP4 mAb (clone 6F12) 20ug 6F12 IEM, ELISA, IF Monoclonal Mouse Sheep, Bovine, Equine, Porcine, Human
BDX-4BDX-1805 Anti-AKAP4 mAb (clone 10F8) 100ug 10F8 IF, IHC, WB     Dog, Goat, Porcine, Rabbit
BDX-4BDX-1805S Anti-AKAP4 mAb (clone 10F8) 20ug 10F8 IF, IHC, WB     Dog, Goat, Porcine, Rabbit
BDX-4BDX-18K12RUO proAKAP4 ELISA Kit (Rodent & Human) 1Kit         Human, Mouse, Rat, Guinea Pig, Hamster
BDX-4BDX-18K8 proAKAP4 4MID Kit (ELISA, Mouse) 1Kit         Mouse
BDX-4BDX-18K8BB proAKAP4 4MID BB Kit (ELISA, Mouse) 1Kit         Mouse
BDX-4BDX-1901 Anti-pro AKAP4 mAb (clone 5H2) 100ug 5H2 IF, IHC, FC, WB     Human, Mouse, Porcine
BDX-4BDX-1901S Anti-pro AKAP4 mAb (clone 5H2) 20ug 5H2 IF, IHC, FC, WB     Human, Mouse, Porcine
BDX-4VDX-18K2 proAKAP4 4MID Kit (ELISA, Pig) 1Kit         Porcine
BDX-4VDX-18K3 proAKAP4 4MID Kit (ELISA, Horse) 1Kit         Equine
BDX-4VDX-18K3BB proAKAP4 4MID BB Kit (ELISA, Horse) 1Kit         Equine
BDX-4VDX-18K4 proAKAP4 4MID Kit (ELISA, Bull) 1Kit         Bovine
BDX-4VDX-18K5 proAKAP4 4MID Kit (ELISA, Dog) 1Kit         Dog
BDX-4VDX-18K5BB proAKAP4 4MID BB Kit (ELISA, Dog) 1Kit         Dog
BDX-4VDX-18K6 proAKAP4 4MID Kit (ELISA, Rabbit) 1Kit         Rabbit
BDX-4VDX-18K7 proAKAP4 4MID Kit (ELISA, Ram) 1Kit         Ram
BDX-4VDX-18K9 proAKAP4 4MID Kit (ELISA, Goat) 1Kit         Goat
BDX-4VDX-19K10 proAKAP4 4MID Kit (ELISA, Feline) 1Kit         Feline
BDX-4VDX-19K11 proAKAP4 4MID Kit (ELISA, Camel) 1Kit         Camel