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The Ram 4MID® Kit (Ref. 4VDX-18K7) is the first immunoassay that validates objectively semen quality by quantifying a protein biomarker named proAKAP4 in samples of ram semen. This ELISA kit is the 1st functional test to evaluate sperm quality.
The dosage of the proAKAP4 biomarker with the Ram 4MID® Kit, will bring you:
- Objective information on semen quality
- Qualification of each breeding male and their ejaculates
- Pertinent parameter to improve sperm cryopreservation
- A functional indicator correlated to fertility index
The concentration of the proAKAP4 biomarker is a good indicator of sperm quality and of how the spermatozoa can keep its motility high and efficient up to fecondation (Sergeant et al., 2019).
Reagents provided in the Ram 4MID® Kit
The complete proAKAP4 ELISA kit (Ref. 4VDX-18K7) called Ram 4MID kit contains all reagents and buffers required for the quantification of proAKAP4 protein in ram semen.
- One Microplate pre-coated with the Capture Antibody
- One vial of Standard
- One vial of Detection Antibody
- One bottle of the Enzyme Reagent
- One bottle of 10X Wash Buffer
- One bottle of the Sample Diluant
- One bottle of the Acid Stop Solution
- One Vial of Positive Control
- Two adhesive plate sealers
| Documents & Links for proAKAP4 4MID Kit (ELISA, Ram) | |
| Manual | proAKAP4 4MID Kit (ELISA, Ram) Manual |
| Vendor Page | proAKAP4 4MID Kit (ELISA, Ram) at 4BioDx |
| Documents & Links for proAKAP4 4MID Kit (ELISA, Ram) | |
| Manual | proAKAP4 4MID Kit (ELISA, Ram) Manual |
| Vendor Page | proAKAP4 4MID Kit (ELISA, Ram) |
| Citations for proAKAP4 4MID Kit (ELISA, Ram) – 6 Found |
| Riesco et al. 2022. Establishment of innovative biomarkers to optimize cooling and cryopreservation protocols in ram sperm. Reproduction in Domestic Animals. 57(1):101. Journal |
| Carracedo et al. 2020. The sperm specific proAKAP4 polypeptide exhibited conserved functions, localizations and metabolism among mammals.. Animal Reproduction Science. 220:106448. Journal |
| Riesco et al. 2020. ProAKAP4 as Novel Molecular Marker of Sperm Quality in Ram: An Integrative Study in Fresh, Cooled and Cryopreserved Sperm. Biomolecules. 10(7):1046. PubMed, Journal |
| Sergeant et al. 2019. The sperm specific protein proAKAP4 as an innovative marker to evaluate sperm quality and fertility. Journal of Dairy & Veterinary Sciences. 11:43466. Journal |
| Delehedde et al. 2018. Concentration of proAKAP4 as a pertinent read-out of sperm quality in mammals. . Animal Reproduction Science. . 194:24. Journal |
| Sergeant et al. 2016. Investigating proteomic methods and tools to assess sperm quality. Animal Reproduction Science . 169: 99–135. Journal |