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The Rabbit 4MID® Kit (Ref. 4VDX-18K6) is the first immunoassay that validates objectively semen quality by quantifying a protein biomarker named proAKAP4 in samples of rabbit semen. This ELISA kit is the 1st functional testto evaluate sperm quality.
The dosage of the proAKAP4 biomarker with the Rabbit 4MID® Kit, will bring you:
- Objective information on semen quality
- Qualification of each breeding male and their ejaculates
- Pertinent parameter to improve sperm cryopreservation
- A functional indicator correlated to fertility and prolificity
The concentration of the proAKAP4 biomarker is a good indicator of sperm quality and of how the spermatozoa can keep its motility high and efficient up to fecondation (Sergeant et al., 2019).
Reagents provided the Rabbit 4MID® Kit
The Rabbit 4MID® Kit (Ref. 4VDX-18K6) contains all reagents and buffers required for the quantification of proAKAP4 protein in rabbit semen.
- One Microplate pre-coated with the Capture Antibody
- One vial of Standard
- One vial of Detection Antibody
- One bottle of the Enzyme Reagent
- One bottle of 10X Wash Buffer
- One bottle of the Sample Diluant
- One bottle of the Acid Stop Solution
- One vial of Positive Control
- Two adhesive plate sealers
| Documents & Links for proAKAP4 4MID Kit (ELISA, Rabbit) | |
| Manual | proAKAP4 4MID Kit (ELISA, Rabbit) Manual |
| Vendor Page | proAKAP4 4MID Kit (ELISA, Rabbit) at 4BioDx |
| Documents & Links for proAKAP4 4MID Kit (ELISA, Rabbit) | |
| Manual | proAKAP4 4MID Kit (ELISA, Rabbit) Manual |
| Vendor Page | proAKAP4 4MID Kit (ELISA, Rabbit) |
| Citations for proAKAP4 4MID Kit (ELISA, Rabbit) – 4 Found |
| Carracedo et al. 2020. The sperm specific proAKAP4 polypeptide exhibited conserved functions, localizations and metabolism among mammals.. Animal Reproduction Science. 220:106448. Journal |
| Sergeant et al. 2019. The sperm specific protein proAKAP4 as an innovative marker to evaluate sperm quality and fertility. Journal of Dairy & Veterinary Sciences. 11:43466. Journal |
| Delehedde et al. 2018. Concentration of proAKAP4 as a pertinent read-out of sperm quality in mammals. . Animal Reproduction Science. . 194:24. Journal |
| Sergeant et al. 2016. Investigating proteomic methods and tools to assess sperm quality. Animal Reproduction Science . 169: 99–135. Journal |