Anti-pro AKAP4 mAb (clone 6F12)

4BioDx

Catalog No.: BDX-4BDX-1701S

Product Description

Mouse monoclonal antibody clone 6F12 recognizes the prodomain of A-kinase anchor protein 4 (AKAP4) and is suitable for Western Blot, Immunofluorescence, ELISA and Immunohistochemistry.

Pro-AKAP4 is processed into mature AKAP4 by proteolytic cleavage of the amino-terminal prodomain (see picture).

AKAP4 (A-Kinase Anchor Protein 4) protein belongs to the family of A-kinase anchor proteins (AKAPs) all sharing a common function of binding to the regulatory subunit of protein kinase A (PKA) and confining the PKA holoenzyme to discrete locations within the cell.

The human AKAP4 gene is located on chromosome X and encodes a 854 amino acid polypeptide localized in the fibrous sheath of the sperm flagellum, AKAP4 plays a major role in sperm motility.

AKAP4 is also named AKAP-4, AKAP82 (A-Kinase Anchor Protein 82 KDa), PRKA4 (Protein Kinase A Anchoring Protein 4), HI, CT99 (Cancer/Testis Antigen 99), FSC1 (Fibrous sheath component 1) or P82.

The monoclonal antibody (clone 6F12) only recognizes the pro-form of AKAP4. It does not react with the cleaved forms AKAP4.

Western blotting and immunocytochemistry techniques using the monoclonal anti-pro AKAP4 antibody clone 6F12 showed that the pro-AKAP4 is specifically expressed in human, equine, porcine, bovine murine and ovine spermatozoa.

Mouse monoclonal antibody clone 6F12 is suitable for Western Blot, Immunofluorescence, ELISA and Immunohistochemistry (see applications).

Product Specifications

• Application: IEM, ELISA, IF
• Reactivity: Sheep, Bovine, Equine, Porcine, Human
• Clonality: Monoclonal (Clone No.: 6F12)
• Host: Mouse

Citations for Anti-pro AKAP4 mAb (clone 6F12) – 8 Found

Dordas-Perpinyà, Marta; Sergeant, Nicolas; Ruelle, Isabelle; Bruyas, Jean-François; Charreaux, Frédéric; Michaud, Sandrine; Carracedo, Sara; Catalán, Jaime; Miró, Jordi; Delehedde, Maryse; Briand-Amirat, Lamia. ProAKAP4 Semen Concentrations as a Valuable Marker Protein of Post-Thawed Semen Quality and Bull Fertility: A Retrospective Study. Veterinary Sciences. 2022;9(5) 

Benderradji, Hamza; Barbotin, Anne-Laure; Leroy-Billiard, Maryse; Prasivoravong, Julie; Marcelli, François; Decanter, Christine; Robin, Geoffroy; Mitchell, Valérie; Rigot, Jean-Marc; Bongiovanni, Antonino; Sauve, Florent; Buée, Luc; Maurage, Claude-Alain; Cartigny, Maryse; Villers, Arnauld; Prevot, Vincent; Catteau-Jonard, Sophie; Sergeant, Nicolas; Giacobini, Paolo; Pigny, Pascal; Leroy, Clara. Defining Reference Ranges for Serum Anti-Müllerian Hormone on a Large Cohort of Normozoospermic Adult Men Highlights New Potential Physiological Functions of AMH on FSH Secretion and Sperm Motility. The Journal Of Clinical Endocrinology And Metabolism. 2022;107(7):1878-1887. 

Malo, Clara; Carracedo, Sara; Delehedde, Maryse; Sergeant, Nicolas; Skidmore, Julian Alexandra. Identification of proAKAP4 concentration variations in dromedary sperm and their correlation with monthly semen parameters. Reproduction & Fertility. 2021;2(4):268-279. 

Blommaert, D; Sergeant, N; Delehedde, M; Donnay, I; Lejeune, J P; Franck, T; Serteyn, D. First results about ProAKAP4 concentration in stallion semen after cryopreservation in two different freezing media. Cryobiology. 2021;102( 34302806):133-135. 

Griffin, Róisín A; Swegen, Aleona; Baker, Mark; Aitken, Robert John; Skerrett-Byrne, David A; Silva Rodriguez, Antonio; Martín-Cano, Francisco E; Nixon, Brett; Peña, Fernando J; Delehedde, Maryse; Sergeant, Nicolas; Gibb, Zamira. Mass spectrometry reveals distinct proteomic profiles in high- and low-quality stallion spermatozoa. Reproduction (Cambridge, England). 2020;160(5):695-707. 

Langlade, Camille; Buff, Samuel; Dias, Catia; Commin, Loris. Assessment of Optimized Frozen/Thawed Semen Samples in Canines with the New A-Kinase Anchor Protein 4 Precursor Biomarker. Biopreservation And Biobanking. 2020;18(5):409-414. 

Blommaert, D; Sergeant, N; Delehedde, M; Jouy, N; Mitchell, V; Franck, T; Donnay, I; Lejeune, J P; Serteyn, D. Expression, localization, and concentration of A-kinase anchor protein 4 (AKAP4) and its precursor (proAKAP4) in equine semen: Promising marker correlated to the total and progressive motility in thawed spermatozoa. Theriogenology. 2019;131( 30947075):52-60. 

Nixon, Brett; Bernstein, Ilana R; Cafe, Shenae L; Delehedde, Maryse; Sergeant, Nicolas; Anderson, Amanda L; Trigg, Natalie A; Eamens, Andrew L; Lord, Tessa; Dun, Matthew D; De Iuliis, Geoffry N; Bromfield, Elizabeth G. A Kinase Anchor Protein 4 Is Vulnerable to Oxidative Adduction in Male Germ Cells. Frontiers In Cell And Developmental Biology. 7( 31921838):319.