Abs For Paraffin Embedded Tissue

  • Figure 1. Western blotting of purified human aorta versican (primarily a mixture of V1 and V2 isoforms) resolved by SDS-PAGE on 3-8% linear gradient gels under reducing conditions (MW, HiMark Unstained Protein Standard) before (Intact) and after combined chondroitinase ABC and endo-b-galactosidase pretreatment (Digested). * Banding pattern observed by immunoblotting depends upon the isoforms and is often complex. In the intact forms, i.e. without removal of GAGs, V0, V1 and V2 do not enter acrylamide gels and therefore agarose gels are recommended. Following chondroitinase-digestion and extensive enzymatic deglycosylation, most isoforms still show complex, smeared banding patterns.

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    Anti Versican (VCAN) mAb (Clone 6B10)

    Catalog No.(s): CAC-PRPG-VS-M04

    Catalog numbers beginning with "CAC" are antibodies from our exclusive Cosmo Bio Antibody Collection. Visit the CAC Antibody homepage to browse the collection list, organized by research topic.Click here to browse a well organized list of...

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  • Figure 1. Western blotting of purified human aorta versican resolved by SDS-PAGE on 3-8% linear gradient gels (MW, HiMark Unstained Protein Standard) before (Intact) and after combined chondroitinase ABC and endo-b-galactosidase pretreatment (Digested). * Banding pattern observed by immunoblotting depends upon the isoforms and is often complex. In the intact forms, i.e. without removal of GAGs, V0, V1 and V2 do not enter acrylamide gels and therefore agarose gels are recommended. Following chondroitinase-digestion and extensive enzymatic deglycosylation, most isoforms still show complex, smeared banding patterns.

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    Anti Versican (VCAN) mAb (Clone 2B3)

    Catalog No.(s): CAC-PRPG-VS-M03

    Catalog numbers beginning with "CAC" are antibodies from our exclusive Cosmo Bio Antibody Collection. Visit the CAC Antibody homepage to browse the collection list, organized by research topic.Click here to browse a well organized list of...

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  • Figure 1. Immunoblotting of intact versican (mixture of V1 and V2 isoforms) in its untreated and chondroitinase ABC (Chase ABC)-digested form, or after combined digestion with chondroitinase ABC and a number of exo- and endoglycosydases. The proteoglycan was resolved by SDS-PAGE under reducing conditions on 3-8% linear gradient gels (MW, HiMark Unstained Protein Standard).

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    Anti Versican/CSPG2 (VCAN) mAb (Clone 5C12)

    Catalog No.(s): CAC-PRPG-VS-M01

    Catalog numbers beginning with "CAC" are antibodies from our exclusive Cosmo Bio Antibody Collection. Visit the CAC Antibody homepage to browse the collection list, organized by research topic.Click here to browse a well organized list of...

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  • Immunostaining of malignant glioblastoma A) and cutaneous melanoma C) lesions. B) Western blot of whole tissue/cell lysates of a human glioblastoma lesion and the human melanoma A375 cell line after SDS-PAGE under reducing conditions on 4-10% gradient gels. D) Immunostaining of human melanoma MeWo cells. Nuclear counterstaining was performed with Hoescht.

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    Anti NG2 (CSPG4) mAb (Clone 2161D7)

    Catalog No.(s): CAC-PRPG-NG-M01

    Catalog numbers beginning with "CAC" are antibodies from our exclusive Cosmo Bio Antibody Collection. Visit the CAC Antibody homepage to browse the collection list, organized by research topic.Click here to browse a well organized list of...

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  • Figure 1. Western blotting on intact (left) and chondroitinase ABC-digested (right) bovine decorin after SDS-PAGE on 8% gels.

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    Anti Decorin (DCN) mAb (Clone 889C7)

    Catalog No.(s): CAC-PRPG-DC-M01

    Catalog numbers beginning with "CAC" are antibodies from our exclusive Cosmo Bio Antibody Collection. Visit the CAC Antibody homepage to browse the collection list, organized by research topic. Click here to browse a well organized list of...

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  • Figure 1. Immunoprecipitation of byglycan from human smooth muscle cells and Western blotting with a second anti-biglycan mAb on the intact (right) and chondroitinase ABC predigested (left) precipitate resolved by SDS-PAGE on gradient 4-18% gels.

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    Anti Biglycan (BGN) mAb (Clone 905A7)

    Catalog No.(s): CAC-PRPG-BG-M01

    Catalog numbers beginning with "CAC" are antibodies from our exclusive Cosmo Bio Antibody Collection. Visit the CAC Antibody homepage to browse the collection list, organized by research topic. Click here to browse a well organized list of...

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  • Figure 1. Immunoblotting of a human articular cartilage aggrecan preparation following SDS-PAGE on 3-8% linear gradient gels under reducing conditions, prior to (Intact) and after combined chondroitinase ABC and keratanase II digestion (Digested).

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    Anti Aggrecan (ACAN) mAb (Clone 5G2)

    Catalog No.(s): CAC-PRPG-AG-M03

    Catalog numbers beginning with "CAC" are antibodies from our exclusive Cosmo Bio Antibody Collection. Visit the CAC Antibody homepage to browse the collection list, organized by research topic.Click here to browse a well organized list of...

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  • Figure 1. SDS-PAGE on 3-8% linear gradient gels under reducing conditions of purified human articular cartilage aggrecan prior to (intact) and after combined keratanase I, endo-galactosidase and chondroitinase ABC-digestion.

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    Anti Aggrecan (ACAN) mAb (Clone 5D3)

    Catalog No.(s): CAC-PRPG-AG-M02

    Catalog numbers beginning with "CAC" are antibodies from our exclusive Cosmo Bio Antibody Collection. Visit the CAC Antibody homepage to browse the collection list, organized by research topic.Click here to browse a well organized list of...

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  • Figure 1. Western blotting of purified human cartilage aggrecan. (A) SDS-agarose gel electrophoresis (0.5%) before and after combined chondroitinase ABC digestion of aggrecan. (B) SDS-PAGE on 3-8% linear gradient gels, after the combined digestion of aggrecan indicated in the legend.

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    Anti Aggrecan (ACAN) mAb (Clone 6F4)

    Catalog No.(s): CAC-PRPG-AG-M01

    Catalog numbers beginning with "CAC" are antibodies from our exclusive Cosmo Bio Antibody Collection. Visit the CAC Antibody homepage to browse the collection list, organized by research topic.Click here to browse a well organized list of...

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  • Figure 1. Characterization of Neurocan peptides pAb and Neurocan peptides mAb (clone 1G2) with samples obtained from 10-day-old rat brain. The PBS-soluble brain CSPG mixture of 10-day-old rats (lane 1) and the brain PBS-extract digested with chondroitinase ABC (lanes 2 and 3) were separated by SDS-PAGE. Neurocan peptides pAb recognized a very diffuse band with the same mobility as that recognized by Neurocan peptides mAb (clone 1G2). Monoclonal antibody (clone 1G2) recognized both 220 and 150 kDa core glycoproteins in the chondroitinase-digested sample. Polyclonal antibody recognized not only the 220 kDa core glycoprotein but also that with a molecular weight of 130 kDa in both (Ref.3).

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    Anti Neurocan (NCAN) Peptides pAb

    Catalog No.(s): CAC-NU-07-005

    Catalog numbers beginning with "CAC" are antibodies from our exclusive Cosmo Bio Antibody Collection. Visit the CAC Antibody homepage to browse the collection list, organized by research topic.Click here to browse a well organized list of...

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  • Figure 1. Characterization of the polyclonal anti-N-syndecan antiserum. Western blot using anti-N-syndecan of a partially purified preparation of membrane-bound proteoglycan (lane 1), and that digested with chondroitinase ABC (lane 2), and heparitinase I (lane 3). After the heparitinase I treatment , the immunoreactive band is sifted to an apparent molecular weight of approximately 140 kDa, identical to that of the core glycoprotein of rat N-syndecan.

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    Anti N-Syndecan (SDC3) pAb

    Catalog No.(s): CAC-NU-07-004

    Visit our Neurodegeneration Products homepage to browse related research productsClick here to browse a well organized list of products for Bone, Collagen, and Extracellular Matrix research.Catalog numbers beginning with "CAC" are antibodies...

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  • Characterization of the monoclonal anti-neuroglycan C antibody (clone C1). Immunoblot analysis of anti-neuroglycan C antibody on partially purified membrane-bound proteoglycans from brains of 10-day-old rats before (lane 1) and after digestion with chondroitinase ABC (CHase ABC; lane 2). [from: J. Biol. Chem. (1995) 270:26876-26882.]

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    Anti Neuroglycan C (CSPG5) mAb (Clone C1)

    Catalog No.(s): CAC-NU-07-003

    Catalog numbers beginning with "CAC" are antibodies from our exclusive Cosmo Bio Antibody Collection. Visit the CAC Antibody homepage to browse the collection list, organized by research topic.Click here to browse a well organized list of...

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  • Characterization of neurocan peptide pAb and neurocan mAb (clone 1G2) with samples obtained from 10-day-old rat brain. PBS-soluble brain CSPG mixture of 10-day-old rats (lane 1) and brain PBS-extract digested with chondroitinase ABC (lanes 2 and 3) were separated by SDS-PAGE. Neurocan peptide pAb recognized a very diffuse band with the same mobility as that recognized by neurocan mAb (clone 1G2). Monoclonal antibody (clone 1G2) recognized both 240 and 150 kDa core glycoproteins in the chondroitinase-digested sample. The polyclonal antibody recognized not only the 240 kDa core glycoprotein but also one with a molecular weight of 130 kDa. [from: Neurochem. Int. (1994) 25:425-431.]

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    Anti Neurocan (NCAN) mAb (Clone 1G2)

    Catalog No.(s): CAC-NU-07-002

    Catalog numbers beginning with "CAC" are antibodies from our exclusive Cosmo Bio Antibody Collection. Visit the CAC Antibody homepage to browse the collection list, organized by research topic.Click here to browse a well organized list of...

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