Food poisoning caused by pathogenic Escherichia coli (diarrheagenic Escherichia coli) is an infectious food poisoning that develops when pathogenic Escherichia coli is taken into the intestinal tract and proliferates due to food intake. This pathogenic Escherichia coli is roughly classified into 5 types. Of these, enterohemorrhagic Escherichia coli causes hemorrhagic colitis due to the verotoxin (VT) produced by them, and rarely causes hemolytic uremic syndrome (HUS) and encephalopathy, resulting in death. It is considered to be the most important in terms of food hygiene among pathogenic Escherichia coli because of the fact that cases of large-scale food poisoning have been reported in the past. In recent years, an increasing number of cases of serotype O26 being isolated from patients with enterohemorrhagic Escherichia coli infection have been observed, and it is the serotype with the second largest number of patients after O157. In addition, enterohemorrhagic Escherichia coli O111 is the second most frequently detected overseas after O157, and it is reported that it is the third most common in Japan after O157 and O26.
Features
- It is a simple one-step operation, so no proficiency is required.
- Get results quickly.
- No special detector is required.
- E. coli O157 detection kit (product number: 999100000) has obtained AOAC-RI PTM SM certification (*).
* AOAC: Various analytical methods for foods, pharmaceuticals, fertilizers / feeds, cosmetics, etc.
* PTM: Performance Tested Methods
Contents
A: Test strip 2 test x 10 Packaging
B: Instruction manual 1 copy
C: Vinyl pouch bag 1
Detection Principal
(1) The antigen in the sample and the gold colloid-labeled antibody bind to each other by the antigen-antibody reaction (form a complex)
(2) The complex moves in the nitrocellulose membrane by the capillary phenomenon
(3) Solid phase to the determination unit (T) The antibody and complex against the converted target substance are bound by an antigen-antibody reaction.
⇒ A reddish-purple color is developed due to the concentration of
gold colloids. Colloid-labeled antibody binds due to antigen-antibody reaction
⇒ Reddish purple color develops in judgment part (C)
Test Results
 
Sample dropping part: The part where the sample is dropped (be careful not to touch it)
b. Reagent-containing part: The part where the reagent required for the reaction is contained
c. Expanding part: Nitrocellulose membrane through which the sample flows (scratches) Be careful not to attach it)
d. Absorption pad: Part where excess sample solution is absorbed
It is possible to write the sample name etc.
e. Measurement item description position: Describe the measurement target of the kit
f. Test line appearance position: Magenta line If is found, it is judged as positive
. G. Control line appearance position: Check the development of the sample. A magenta line always appears.
Handling Precautions
Be sure to read the instruction manual before using this kit.
This kit is a reagent for detecting microorganisms in food and has not been approved as an in vitro diagnostic agent.
Be sure to check the samples that are positive with this kit by other methods.
When conducting a test, in order to prevent infection, perform it in an appropriate facility / facility and follow standard microbiological testing procedures.
Appropriately sterilize and dispose of the test strips, enrichment medium, samples, and the rest of the sample solution used for the test.
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