| BioMasher I | BioMasher II | BioMasher III | BioMasher V | BioMasher SP | |
|---|---|---|---|---|---|
| Features | Multiple preps at one time. | For high yield | For high yield and multple preps at a time | For high yield and multple preps at a time | For high yield |
| Collection Amount | Small | Large | Large | Large | Large |
| Produces Heat | No | Very little | Very little | Very little | Very little |
| Centrifuge Required | Yes | No | Yes | Yes | No |
| Soft Tissue | Excellent | Excellent | Excellent | Excellent | Excellent |
| Hard Tissue | Fair | Excellent | Excellent | Excellent | Excellent |
BioMasher I consists of a filter tube and a pestle. The sample is placed in the filter tube and the pestle is inserted from above. Centrifugal force is applied with a centrifuge allowing the pestle to crush the sample with this force. This allows the sample to pass through the filter of the filter tube, enabling the sample to be disrupted. The fibrous membrane component of the sample will be caught by the filter.
The pestle has a cross-shaped blade at the tip. By pushing and turning the pestle manually, the sample, which is hard to disrupt by centrifugation alone, can be ground.
Variations:
- With O-ring: BioMasher I with an O-ring is suitable for homogenizing soft tissue with centrifugal force alone. In particular, it is suitable for simultaneous processing of many samples and for infectious samples.
- Without O-ring: Hard tissue may not be homogenized sufficiently by centrifugal force alone. The model without an O-ring is suitable when grinding the sample on the filter area before centrifugation to enhance disruption efficiency.
BioMasher II consists of a 1.5 mL microtube and a pestle. The sample and extraction buffer are placed in the tube, and ground to prepare the homogenate. The microtube and pestle are molded so that they fit without any space between them. The texture of the inner wall of the tube and the outer periphery of the tip of the pestle enhances the efficiency of the disruption of the sample. The pestle has a splash guard, which is designed to fit the tube without unnecessary spaces. BioMasher II can be used to disrupt various samples, such as animal tissue/organ, plants (stems, roots, seeds), and insects.
BioMasher III has the features of both BioMasher I and II. This model consists of a filter tube with an abrasive surface inner wall and a pestle with a textured surface. After grinding the sample in the filter tube using the pestle, centrifuge the extraction liquid. The extracted sample will be filtered and caught in the tube. Since the fibrous membrane component get trapped by the filter, BioMasher III is recommended for homogenization of highly fibrous samples.
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