Sperm Quality Assessment
4MID® ProAKAP4 ELISA Kits from 4BioDx offer a path to predictable sperm quality.
Breeders Take Note: Pig. Horse. Bull. Dog. Camel. Ram. Rabbit. Goat. Mouse. Human.
ProAKAP4: A promising biomarker for sperm quality assessment in humans and other mammals.
Investigations of molecular actors through proteomic methods have help to underline mechanisms of male fertility and have generate new methods to improve semen quality and pre-dict fertility. The proAKAP4 precursor is a key protein that is playing crucial roles in sperm physiology. The fertilizing ability of ejaculated spermatozoa that can nowadays being evaluate through the 4MID® kits, that quantify expression levels of proAKAP4 in ejaculate and/or doses. Measuring proAKAP4 level of expressions should generate pertinent information to guide the prognosis of male fertility and prolificity in breeding activities. The 4MID® kits represent a good example of how biotechnology and proteomics tools can get into routine as an innovative tool for breeders. Such examples could lead to the development of other innovative markers to improve sperm quality assessment in competitive breeding industries, animal reproduction clinics and veterinarian practices and being a breakthrough in the field of artificial insemination.
The concentration of the proAKAP4 biomarker is a good indicator of sperm quality and of how the spermatozoa can keep its motility high and efficient up to fecondation (Delehedde et al., 2019).
The Human 4MID® proAKAP4 Kits are complete immunoassays that allows you to quantify human proAKAP4 in sperm samples as a biomarker of sperm quality.
4MID® proAKAP4 ELISA Kit Features and Benefits
- Innovative complete immunoassay to assess semen quality.
- These ELISA Kits are the 1st functional tests to evaluate sperm quality.
- All reagents and buffers required for the quantification of proAKAP4 protein in sperm are included with the kit.
- Works with fresh ejaculate or with frozen semen.
- Easy to use sandwich immunoassay / Manual or automated.
- Robust and reproductible method.
Ordering Information: 4MID® proAKAP4 ELISA Kits
| Specificity | Cat. No. | Name | Test Sample Capacity |
Sample Type |
| Pig | BDX-4VDX-18K2 | Pig proAKAP4 ELISA | 88 | fresh or frozen semen |
| Horse | BDX-4VDX-18K3 | Horse proAKAP4 ELISA | 88 | fresh or frozen semen |
| Horse** | BDX-4VDX-18K3BB | Horse proAKAP4 ELISA BB | 1-24 | fresh or frozen semen |
| Bull | BDX-4VDX-18K4 | Horse proAKAP4 ELISA | 88 | fresh or frozen semen |
| Dog | BDX-4VDX-18K5 | Dog proAKAP4 ELISA | 88 | fresh or frozen semen |
| Dog** | BDX-4VDX18K5BB | Dog proAKAP4 ELISA BB | 1-24 | fresh or frozen semen |
| Rabbit | BDX-4VDX-18K6 | Rabbit proAKAP4 ELISA | 88 | fresh or frozen semen |
| Ram | BDX-4VDX-18K7 | Ram proAKAP4 ELISA | 88 | fresh or frozen semen |
| Mouse | BDX-4BDX-18K8 | Mouse proAKAP4 ELISA | 88 | fresh or frozen semen |
| Mouse** | BDX-4BDX-18K8BB | Mouse proAKAP4 ELISA BB | 1-24 | fresh or frozen semen |
| Goat | BDX-4VDX-18K9 | Goat proAKAP4 ELISA | 88 | fresh or frozen semen |
| Feline | BDX-4VDX-19K10 | Feline proAKAP4 ELISA | 88 | fresh or frozen semen |
| Camel | BDX-4VDX-19K11 | Camel proAKAP4 ELISA | 88 | fresh or frozen semen |
| Human/Rodent | BDX-4VDX-18K12RUO | Human proAKAP4 ELISA | 88 | fresh or frozen semen |
| ** BB kits are formatted for the convenient testing of 2 samples at a time (2 samples per strip), while preserving the remaining strips for later use. With 12 strips per plate, BB format kits allow testing 1-24 semen samples in total. | ||||
Monoclonal Antibodies to AKAP4 and proAKAP4
| Name (click for order infor and datasheet) |
Clonality |
Host | Reactivity | Applications |
| Anti AKAP4 | mAb, clone 7E10 | mouse | sheep bovine equine porcine human |
WB IHC IF ELISA EM
|
| Anti-AKAP4 | mAb, clone 10F8 | mouse | dog rabbit pig ram cat goat | WB IHC |
| Anti-proAKAP4 | mAb, clone 6F12 | mouse | human horse bull mouse ram rat | WB IHC IF |
| Anti-proAKAP4 | mAb, clone 5H2 | mouse | human mouse goat cat dog pig | WB IHC IF |
proAKAP4 Literature References
Delehedde M, Carracedo S, Selleslagh M, et al. ProAKAP4 polypeptide as a biomarker of sperm functionality and male fertility disorders. Int J Gynecol and Reprod Sci. (2019);2(1):13-19.
Delehedde M, Demouveaux B, Remy G, Selleslagh M Dewulf Q, Desseyn JL, Moreau M, Gosset P, Muriel Pichavant M and Sergeant N (2019a) ProAKAP4 concentrations as an indicator of good spermatogenesis and sperm quality under oxidative stress conditions. Andrology. Vol. 7(1):86.
Delehedde M, Demouveaux B, Remy G, Desseyn JL, Mitchell V, Barbotin AL, Gosset P, Pichavant M and Sergeant N (2019b) Evaluation of the proAKAP4 detection kits as functional tests of sperm quality under stress conditions. Human Reproduction. In press.
Jumeau F, Sigala J, Dossou-Gbete F, Frimat K, Barbotin AL, Buée L, Béhal H, Sergeant N and Mitchell V (2018) A-kinase anchor protein 4 precursors (pro-AKAP4) in human sperm. Andrology. Vol. (6):854-859.
Jumeau F, Sigala J, Fernandez-Gomez FJ, Eddarkaoui S, Duban-Deweer S, Buée L, Béhal H, Sergeant N and Mitchell V (2018) Gel electrophoresis of human sperm: a simple method for evaluating sperm protein quality. Basic Clin. Androl. Vol. 3(28):10.
Escalier D and Albert M (2006) New ?brous sheath anomaly in spermatozoa of men with consanguinity. Fertility and Sterility. Vol. 86:2191-2199.
All Products on This Page Are Research Use Only Products (RUO) / Not for Diagnosis or Therapeutic Use.