proAKAP4 4MID Kit (ELISA, Dog)

Catalog No:
BDX-4VDX-18K5
$670.00
Reactivity: Dog 

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The proAKAP4 ELISA kit allows you to quantify proAKAP4 in samples of dog semen as a biomarker of sperm quality.

SPECIFICITY

Dog proAKAP4

ANALYTICAL RANGE

5 ng to 300 ng

SAMPLE TYPE

Dog semen

Innovative complete immunoassay to assess semen quality in dog

The proAKAP4 ELISA kit is the first immunoassay that validates objectively semen quality by quantifying a protein biomarker named proAKAP4 in samples of dog semen. This ELISA Kit is the 1st functional test to evaluate sperm quality.

The dosage of the proAKAP4 biomarker with the ELISA Kit, will bring you:

  • Objective information on semen quality
  • Qualification of each breeding male and their ejaculates
  • Pertinent parameter to improve sperm cryopreservation
  • functional indicator correlated to fertility index

The concentration of the proAKAP4 biomarker is a good indicator of sperm quality and of how the spermatozoa can keep its motility high and efficient up to fecondation.

Technical data

  • 40 samples can be run in duplicate
  • Working with fresh ejaculate or with frozen semen 
  • Easy to use immunoassay / Manual and automated assay
  • Robust and reproductible method

Research Use Only Product (RUO) / Not for Diagnosis or Therapeutic Use.

Reagents provided: The complete proAKAP4 ELISA kit contains all reagents and buffers required for the quantification of proAKAP4 protein in dog semen.

  • One Microplate pre-coated with the Capture Antibody
  • One vial of Standard
  • One vial of Detection Antibody
  • One bottle of the Enzyme Reagent
  • One bottle of 10X Wash Buffer
  • One bottle of the Sample Diluant
  • One bottle of the Acid Stop Solution
  • Three ELISA plate sealers
Documents & Links for proAKAP4 4MID Kit (ELISA, Dog)
Manual proAKAP4 4MID Kit (ELISA, Dog) Manual
Vendor Page proAKAP4 4MID Kit (ELISA, Dog) at 4BioDx

Documents & Links for proAKAP4 4MID Kit (ELISA, Dog)
Manual proAKAP4 4MID Kit (ELISA, Dog) Manual
Vendor Page proAKAP4 4MID Kit (ELISA, Dog)

Citations for proAKAP4 4MID Kit (ELISA, Dog) – 8 Found
Schäfer-Somi et al. 2022. Canine Spermatozoa—Predictability of Cryotolerance . Animals. 12:733.  Journal
Sergeant et al. 2020. Proteolysis of proAKAP4 in semen as a regulatory sensor of sperm quality and functionality. . Andrology. 8(Supp 1):44-45.  Journal
Carracedo et al. 2020. The sperm specific proAKAP4 polypeptide exhibited conserved functions, localizations and metabolism among mammals.. Animal Reproduction Science. 220:106448.  Journal
Le Couazer et al. 2019. ProAKAP4 as a valuable marker to assess sperm quality in dogs.. Reproduction in Domestic Animals. 54(2):91-92.  
Le Couazer et al. 2019. Expression of proAKAP4 in dog semen as promising marker of sperm quality. . Reproduction in Domestic Animal. . 54(S3):73.  Journal
Sergeant et al. 2019. The sperm specific protein proAKAP4 as an innovative marker to evaluate sperm quality and fertility. Journal of Dairy & Veterinary Sciences. 11:43466.  Journal
Delehedde et al. 2018. Concentration of proAKAP4 as a pertinent read-out of sperm quality in mammals. . Animal Reproduction Science. . 194:24.  Journal
Sergeant et al. 2016. Investigating proteomic methods and tools to assess sperm quality. Animal Reproduction Science . 169: 99–135.  Journal