proAKAP4 4MID BB Kit (ELISA, Horse)

4BioDx

Catalog No: BDX-4VDX-18K3BB

Reactivity: Equine 

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BB size: Innovative immunoassay to assess semen quality now available in BB size!

Convenient multi-use format allows you to test 1 or 2 samples at a time, and use the rest of the kit at another time. You can run 1 to 24 samples in total, one strip at the time, then 12 times, as compared to the traditional kit which runs 1-88 samples one time.

Includes more detection antibody, positive control, and uses a shorter standard curve.
 

The proAKAP4 ELISA kit is the first immunoassay that validates objectively semen quality by quantifying a protein biomarker named proAKAP4 in samples of horse semen. This ELISA Kit is the 1st functional test to evaluate sperm quality.

The dosage of the proAKAP4 biomarker with the ELISA Kit will bring you:

• Tool for the selection of the breeding males
• Qualification of each breeding male 
• Qualification of male ejaculates before and after cryopreservation

The concentration of the proAKAP4 biomarker is a good indicator of sperm quality and of how the spermatozoa can keep its motility high and efficient up to fecundation.

Studies are currently conducted together with the "European Horse Center of Mont Le Soie Linalux" (Belgium) and the research teams of SPQI - 4BioDx.

Technical data


• Fewer samples, more time points: You can run 1 to 24 samples in total, one strip at the time, then 12 times

• Working with fresh ejaculate or with frozen semen 

• Easy to use immunoassay / Manual and automated assay

• Robust and reproductible method


Research Use Only Product (RUO) / Not for Diagnosis or Therapeutic Use.

Reagents provided: The complete proAKAP4 ELISA kit contains all reagents and buffers required for the quantification of in horse semen.


• R1 - Capture Antibody Coated Microplate of 96-wells (12 x 8-well strips)

• R2 - 1 Bottle of 10x Washing Buffer Solution (30 mL)

• R3 - 1 Bottle of 1x Dilution Buffer (30 mL)

• R4 - 5 Vials of Ready-to-Use Standard Solution (1.5 mL per vial)

• R5 - 1 Bottle of 1x Dog Spermatozoa Lysis Buffer (30 mL)

• R6 - 6 Vials of Detection Antibody (110 μL)

• R7 - 1 Bottle of Substrate Solution (11 mL)

• R8 - 1 Bottle 1x Stop Solution (6 mL)

• R9 - 1 Vial of Positive Control (1.5 mL)

• R10 - 2 Adhesive Plate Sealers

• R11 - User Manual Instructions






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