Reactivity: Human, Mouse, Porcine
See all IVF and fertility-related products available from Cosmo Bio USA
The anti-AKAP4 mouse monoclonal antibody clone 5H2 recognizes the carboxy-terminal region of A-kinase anchor protein 4 (AKAP4). This antibody is suitable for Western Blot, for Immunohistochemistry, for ELISA, for Electron Microscopy, for Flow cytometry and Immunofluorescence.
This monoclonal antibody was produced from a mouse hybridoma resulting from a mouse immunized with a peptide covering the C-terminus of human AKAP4 protein sequence (Uniprot ref. Q5JQC9) which is 90% homologous between mammals.
This antibody is determined by its ability to recognise the C-terminus human AKAP4 protein. The monoclonal antibody (clone 5H2) recognizes both the pro-form of AKAP4 (110 kDa / 854 AA) and the AKAP4 (82 kDa / 665 AA) but does not react with the prodomain (21 kDa). It reacts also with AKAP4 proteins from horse, bull, mouse, rat and ram semens.
| Documents & Links for Anti-pro AKAP4 mAb (clone 5H2) | |
| Datasheet | Anti-pro AKAP4 mAb (clone 5H2) 100ug Datasheet |
| Datasheet | Anti-pro AKAP4 mAb (clone 5H2) 20ug Datasheet |
| Vendor Page | Anti-pro AKAP4 mAb (clone 5H2) at 4BioDx |
| Documents & Links for Anti-pro AKAP4 mAb (clone 5H2) | |
| Datasheet | Anti-pro AKAP4 mAb (clone 5H2) 100ug Datasheet |
| Datasheet | Anti-pro AKAP4 mAb (clone 5H2) 20ug Datasheet |
| Vendor Page | Anti-pro AKAP4 mAb (clone 5H2) |
| Citations for Anti-pro AKAP4 mAb (clone 5H2) – 7 Found |
| Riesco et al. 2022. Establishment of innovative biomarkers to optimize cooling and cryopreservation protocols in ram sperm. Reproduction in Domestic Animals. 57(1):101. Journal |
| Le Couazer et al. 2021. Are AKAP4 and proAKAP4 Found in Canine Semen? Preliminary Results.. Aspects Nanotechnol . 3(1):70-75. Journal |
| Carracedo et al. 2020. The sperm specific proAKAP4 polypeptide exhibited conserved functions, localizations and metabolism among mammals.. Animal Reproduction Science. 220:106448. Journal |
| Riesco et al. 2020. ProAKAP4 as Novel Molecular Marker of Sperm Quality in Ram: An Integrative Study in Fresh, Cooled and Cryopreserved Sperm. Biomolecules. 10(7):1046. PubMed, Journal |
| Dewulf et al. 2019. The effects of freeze-thaw cycles and of storage time on the stability of proAKAP4 polypeptide in raw sperm samples: implications for semen analysis assessment in breeding activities.. Journal of Dairy & Veterinary Sciences. . 13(3):44743. Journal |
| Delehedde et al. 2018. Concentration of proAKAP4 as a pertinent read-out of sperm quality in mammals. . Animal Reproduction Science. . 194:24. Journal |
| Sergeant et al. 2016. Investigating proteomic methods and tools to assess sperm quality. Animal Reproduction Science . 169: 99–135. Journal |