proAKAP4 4MID Kit (ELISA, Horse)

4BioDx

Catalog No: BDX-4VDX-18K3

Reactivity: Equine 

See all IVF and fertility-related products available from Cosmo Bio USA

Innovative immunoassay to assess semen quality in horse

The proAKAP4 ELISA kit is the first immunoassay that validates objectively semen quality by quantifying a protein biomarker named proAKAP4 in samples of horse semen. This ELISA Kit is the 1st functional testto evaluate sperm quality.

The dosage of the proAKAP4 biomarker with the ELISA Kit will bring you:

• Tool for the selection of the breeding males
• Qualification of each breeding male 
• Qualification of male ejaculates before and after cryopreservation

The concentration of the proAKAP4 biomarker is a good indicator of sperm quality and of how the spermatozoa can keep its motility high and efficient up to fecondation.

Studies are currently conducted together with the "European Horse Center of Mont Le Soie Linalux" (Belgium) and the research teams of SPQI - 4BioDx.

Technical data

• 40 samples can be run in duplicate
• Working with fresh ejaculate or with frozen semen 
• Easy to use immunoassay / Manual and automated assay
• Robust and reproductible method

Research Use Only Product (RUO) / Not for Diagnosis or Therapeutic Use.

Reagents provided: The complete proAKAP4 ELISA kit contains all reagents and buffers required for the quantification of in horse semen.

• One Microplate pre-coated with the Capture Antibody
• One vial of Standard
• One vial of Detection Antibody
• One bottle of the Enzyme Reagent
• One bottle of 10X Wash Buffer
• One bottle of the Sample Diluant
• One bottle of the Acid Stop Solution
• Three ELISA plate sealers

Citations for proAKAP4 4MID Kit (ELISA, Horse) – 12 Found

Kowalczyk et al. 2022. The Concentration of ProAKAP4 and Other Indicators of Cryopotential of Spermatozoa Cryopreserved in Extender with Holothuroidea Extract Addition. Animals (Basel). 12(4):521.   , Journal

Dordas-Perpinya et al. 2022. ProAKAP4 concentrations in stallion and donkey: Comparison with kinetic parameters, motility and concentration. . Reproduction in Domestic Animals. 57(1):61.  Journal

Blommaert et al. 2021. First results about ProAKAP4 concentration in stallion semen after cryopreservation in two different freezing media. Cryobiology. 102:133-35.   , Journal

Sergeant et al. 2020. Proteolysis of proAKAP4 in semen as a regulatory sensor of sperm quality and functionality. . Andrology. 8(Supp 1):44-45.  Journal

Carracedo et al. 2020. The sperm specific proAKAP4 polypeptide exhibited conserved functions, localizations and metabolism among mammals.. Animal Reproduction Science. 220:106448.  Journal

Sergeant et al. 2020. Assessment of the proAKAP4 concentrations as a pertinent sperm parameter to select extenders and preservatives of stallion semen. Animal Reproduction Science. 220:106449.  Journal

Blommaert et al. 2019. Expression, localization, and concentration of A-kinase anchor protein 4 (AKAP4) and its precursor (proAKAP4) in equine semen: promising marker correlated to the total and progressive motility in thawed spermatozoa.. Theriogenology. 131:52-60.   , Journal

Sergeant et al. 2019. The sperm specific protein proAKAP4 as an innovative marker to evaluate sperm quality and fertility. Journal of Dairy & Veterinary Sciences. 11:43466.  Journal

Blommaert et al. 2019. The Horse 4MID® Kit as a promising tool to compare different freezing media to preserve stallion semen. . Rev. Bras. Reprod. Anim.. 43(2):585.  Journal

Delehedde et al. 2018. Concentration of proAKAP4 as a pertinent read-out of sperm quality in mammals. . Animal Reproduction Science. . 194:24.  Journal

Blommaert et al. 2018. Significant correlation between the proAKAP4 concentration and the total and progressive motility in stallion sperm after thawing. . Journal of Equine Veterinary Science. 66:43.  Journal

Sergeant et al. 2016. Investigating proteomic methods and tools to assess sperm quality. Animal Reproduction Science . 169: 99–135.  Journal