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The Mouse and Rat 4MID® Kit (Ref. 4BDX-18K8) contains all reagents and buffers required for the quantification of proAKAP4 protein in mouse and rat sperm. This ELISA Kit is the 1st functional test to evaluate sperm quality.
The concentration of the proAKAP4 biomarker is a good indicator of sperm quality and of how the spermatozoa can keep its motility high and efficient up to fecondation.
Reagents provided in the ProAKAP4 4MID® Kit
The complete proAKAP4 ELISA kit (Ref. 4VDX-18K8) called Ram 4MID kit contains all reagents and buffers required for the quantification of proAKAP4 protein in ram semen.
- One Microplate pre-coated with the Capture Antibody
- One vial of Standard
- One vial of Detection Antibody
- One bottle of the Enzyme Reagent
- One bottle of 10X Wash Buffer
- One bottle of the Sample Diluant
- One bottle of the Acid Stop Solution
- One Vial of Positive Control
- Two adhesive plate sealers
Documents & Links for proAKAP4 4MID Kit (ELISA, Mouse) | |
Manual | proAKAP4 4MID Kit (ELISA, Mouse) Manual |
Vendor Page | proAKAP4 4MID Kit (ELISA, Mouse) at 4BioDx |
Documents & Links for proAKAP4 4MID Kit (ELISA, Mouse) | |
Manual | proAKAP4 4MID Kit (ELISA, Mouse) Manual |
Vendor Page | proAKAP4 4MID Kit (ELISA, Mouse) |
Citations for proAKAP4 4MID Kit (ELISA, Mouse) – 9 Found |
Boersma et al. 2022. Influence of sperm cryopreservation on sperm motility and proAKAP4 concentration in mice. Reprod Med Biol.. 21:e12480. PubMed, Journal |
Delehedde et al. 2020. Cigarette smoking affects the sperm-specific proAKAP4 concentrations and impairs both spermatogenesis and sperm quality. Andrology. 8(Supp 1):45-46. Journal |
Sergeant et al. 2020. Proteolysis of proAKAP4 in semen as a regulatory sensor of sperm quality and functionality. . Andrology. 8(Supp 1):44-45. Journal |
Carracedo et al. 2020. The sperm specific proAKAP4 polypeptide exhibited conserved functions, localizations and metabolism among mammals.. Animal Reproduction Science. 220:106448. Journal |
Delehedde et al. 2019. ProAKAP4 polypeptide as a biomarker of sperm functionality and male fertility disorders. . Int J Gynecol and Reprod Sci. . 2(1):13-19. Journal |
Delehedde et al. 2019. ProAKAP4 concentrations as an indicator of good spermatogenesis and sperm quality under oxidative stress conditions. . Andrology. 7(1):86. Journal |
Sergeant et al. 2019. The sperm specific protein proAKAP4 as an innovative marker to evaluate sperm quality and fertility. Journal of Dairy & Veterinary Sciences. 11:43466. Journal |
Delehedde et al. 2018. Concentration of proAKAP4 as a pertinent read-out of sperm quality in mammals. . Animal Reproduction Science. . 194:24. Journal |
Sergeant et al. 2016. Investigating proteomic methods and tools to assess sperm quality. Animal Reproduction Science . 169: 99–135. Journal |