Written by Dr. Naomi Nakagata, this beautifully bound, full color manual offers step-by-step protocols with illustrative pictures, media composition, and references. It is the perfect companion piece to accompany your mouse IVF work performed with KYD and CSR products.
Available in English and Spanish,and French. Free, no purchase necessary, and free shipping!*
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Reproductive Engeneering Techniques in Mice (3rd Edition)
TABLE OF CONTENTS
Chapter 1 In Vitro Fertilization (IVF)
- 1-1 Preparing and Assembling Pipettes for Embryo Handling p.4
- 1-2 In Vitro Fertilization (IVF) 6 1-3 In Vitro Fertilization (IVF) using Ultra-Superovulation Reagent p.12
Chapter 2 Transportation of sperm
- 2-1 Collection and Transport at Cold Temperature of Cauda Epididymis p.14
- 2-2 In Vitro Fertilization using Epididymal Sperm Transported at Cold Temperature p.18
Chapter 3 Cryopreservation of sperm
- 3-1 Cryopreservation of Mouse Spermatozoa p.20
- 3-2 In Vitro Fertilization using Cryopreserved Spermatozoa p.26
- 3-3 Rescue In Vitro Fertilization Method for Legacy Stock of Cryopreserved Spermatozoa p.32
Chapter 4 Preparation of Oocyte & Embryo
- 4-1 Preparation of Laser-microdissected Oocytes p.36
- 4-2 Partial Zona Dissection (PZD) p.39
- 4-3 Collecting 2-Cell Stage Embryos p.42
Chapter 5 Transportation of Oocyte & Embryo
- 5-1 Transport of 2-Cell Embryos at Cold Temperature p.46
- 5-2 Transportation of Mouse Oviducts Containing 2-Cell Embryos at Low Temperature p.52
Chapter 6 Cryopreservation of Oocyte & Embryo
- 6-1 Simple Vitrification of Mouse Embryos p.54
- 6-2 Simple Vitrification of Mouse Oocytes p.59
- 6-3 Vitrification and Transplantation of Mouse Ovaries p.62
Chapter 7 Other Techniques
- 7-1 Vasectomy for the Creation of Sterile Males p.64
- 7-2 Embryo Transfer into the Oviduct p.66
- 7-3 Embryo Transfer into the Uterus p.72
- 7-4 Caesarean Section and Fostering p.76
Chapter 8 Media
- 8-1 Storage of Media and Solutions in Ampoules Under Nitrogen Gas p.78
- 8-2 Tables of Media Composition p.79
All material:Copyright © 2015 by Naomi Nakagata
| Documents & Links for Reproductive Engineering Techniques in Mice Technical Manual | |
| Manual | KYD Catalog (2023 ver) |
| Documents & Links for Reproductive Engineering Techniques in Mice Technical Manual | |
| Manual | KYD Catalog (2023 ver) |