Collagen AGEs Assay Kit, CML-Specific, Glyoxal

Cosmo Bio LTD

Catalog No: CSR-AAS-AGE-K02

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Although carbohydrates are indispensable for ATP production, excess amounts of carbohydrates modify amino residues of amino acids such as lysine and arginine, and results in the irreversible functional disorders of proteins by changingthe three-dimensional structure and net negative charge in patients with disordered metabolism. Since this reaction was first reported by Louis Camille Maillard in 1912, the reaction is called the Maillard reaction, or glycation. The Maillard reactionis divided by early and advanced stages. Early stage

generates Amadori rearrangement products, such as haemoglobin A1c, whereas advanced stage generates the

AGEs (advanced glycation end products), which is characterized by colour in brown andprotein

cross-linking. Collagen, the structural protein that forms skin, blood vessel wall and bone, also undergo

glycation reaction.

CML is generated by the oxidative cleavage of Amadori products by hydroxyl radicals (1), peroxynitrite

(2)and hypochloric acid (3), thus suggesting that CML is an important biological marker of oxidative stress in vivo. CML concentration, adjusted for age and duration of diabetes, is further increased in patients who have severe complications, includingnephropathy (4), retinopathy (5), and atherosclerosis (6).

CML ELISA Assay Kit provides rapid detection of CML formed by glycation with glyoxal on the collagen

coating plate. This kit is suitable to the research for functional foods and cosmeticmaterials which have anti-glycation activity.

Principle: Collagen coated on 96-well plate is glycated by glyoxal and CML is formed onto collagen coating wells for 24hrs at 37 °C. The inhibitory effects of glycation by aminoguanidin (positive control) or samples are probed with an anti-CML antibody, followed by an HRP conjugated secondary antibody. The inhibitory effect of glycation in unknown sample is determined by comparing with the inhibitory effect of glycation by aminoguanidin (positive control).

“Collagen AGEs Assay Kit, CML-Specific, Glyoxal” is suitable to the research for functional foods and cosmetic materials which have an anti-glycation activity.