Collagen AGEs Assay Kit, CML-Specific, Glyoxal

Cosmo Bio

Catalog No.: CSR-AAS-AGE-K02

Product Description

Although carbohydrates are indispensable for ATP production, excess amounts of carbohydrates modify amino residues of amino acids such as lysine and arginine, and results in the irreversible functional disorders of proteins by changingthe three-dimensional structure and net negative charge in patients with disordered metabolism. Since this reaction was first reported by Louis Camille Maillard in 1912, the reaction is called the Maillard reaction, or glycation. The Maillard reactionis divided by early and advanced stages. Early stage generates Amadori rearrangement products, such as haemoglobin A1c, whereas advanced stage generates the AGEs (advanced glycation end products), which is characterized by colour in brown andprotein cross-linking. Collagen, the structural protein that forms skin, blood vessel wall and bone, also undergo glycation reaction. This Collagen AGEs Assay Kit, CML-Specific provides rapid detection of CML formed by glycation with glyoxal on the collagen coating plate. This kit is suitable to the research for functional foods and cosmeticmaterials which have anti-glycation activity.

Principle: Collagen coated on 96-well plate is glycated by glyoxal and CML is formed onto collagen coating wells for 24hrs at 37 °C. The inhibitory effects of glycation by aminoguanidin (positive control) or samples are probed with an anti-CML antibody, followed by an HRP conjugated secondary antibody. The inhibitory effect of glycation in unknown sample is determined by comparing with the inhibitory effect of glycation by aminoguanidin (positive control).

Features

• Specific detection of CML (Nε-carboxymethyllysine) by ELISA
• Ideal for screening AGE formation inhibitors
• Useful for the development of functional food ingredients and cosmetic materials with anti-glycation activity

Product Data

Fig 1. Screening for anti-glycation compounds using CML generated from collagen glycated with glyoxal for one day. Concentrations indicate the final concentration in the well. 

AGEs Research & Anti-Glycation Kit Selection Guide

Fig 2. AGEs formation pathway

Figure	Target	Recommended Kit (Product name / Cat.No.)
1	Glucose/Fructose-Induced AGE Formation*	Collagen Glycation Assay Kit, Glucose/Fructose  (PMC-AK70)
2-4	Glyceraldehyde-Induced AGE Formation*	Collagen Glycation Assay Kit, Glyceraldehyde  (PMC-AK71) Albumin Glycation Assay Kit, Glyceraldehyde  (CSR-AAS-AGE-K01) Elastin Glycation Assay Kit, Glyceraldehyde  (CSR-AAS-AGE-K05)
5	CML (Nε-Carboxymethyllysine)	Collagen AGEs Assay Kit, CML-Specific, Glyoxal  (CSR-AAS-AGE-K02)
6	CMA (Nω-Carboxymethylarginine)	Collagen AGEs Assay Kit, CMA-Specific, Glyoxal  (CSR-AAS-AGE-K03)
7	RAGE-reactive AGEs	RAGE Reactive AGEs Assay Kit, Glyceraldehyde  (CSR-AAS-AGE-K04)

* Select the assay according to the target protein or glycation model:
• PMC-AK70: Collagen (glucose/fructose-induced glycation)
• PMC-AK71: Collagen (glyceraldehyde-induced glycation)
• CSR-AAS-AGE-K01: Albumin
• CSR-AAS-AGE-K05: Elastin