Collagen Glycation Assay Kit, Glyceraldehyde

Cosmo Bio

Catalog No.: PMC-AK71

Product Description

For rapid detection of fluorescent AGEs and inhibition assay for glycation of collagen by glyceraldehyde. The non-enzymatic reaction of reducing carbohydrates with lysine or arginine side chains and N-terminal amino groups of macromolecules (proteins, phospholipids and nucleic acids) is called the Maillard reaction or glycation. Theproducts of this process, termed advanced glycation end products (AGEs), adversely affect the functional properties of proteins. Many AGEs have fluorescent and convalent cross-linking properties. Accumulation of AGEs has been thought to play an important role in the pathogenesis of diabetic patients as well as the aging process.

Recent studies have suggested that AGEs can arise not only from sugars but also from carbonyl compounds derived from the autoxidation of sugars and other metabolic pathways. Among different AGEs, there is evidence that glyceraldehyde -derived AGEs are associated with such cytotoxcity.

Collagen Anti-glycation Assay Kit, Glyceraldehyde provides rapid detection of fluorescent AGEs and inhibition assay forglycation of collagen by glyceraldehyde. This kit provides sufficient reagents to perform up to 192 assays.

Principle: Collagen Anti-glycation Assay Kit, Glyceraldehyde is a complete assay system designed to measure the fluorescent AGEs usingthe fluorescence microplate reader equipped with a 370nm excitation filter and 440nm emission filter.

Collagen Glycation Assay Kit, Glyceraldehyde FAQs

What is the molecular weight of aminoguanidine included in Collagen Glycation Assay Kit, Glucose/Fructose (Cat. No. PMC-AK70) and Collagen Glycation Assay Kit, Glyceraldehyde (Cat. No. PMC-AK71)? Is it supplied as a hydrochloride salt?
The molecular weight of aminoguanidine is 110.55. It is supplied as the hydrochloride salt.

What is the mechanism by which aminoguanidine exhibits anti-glycation activity?
Aminoguanidine inhibits glycation by binding its amino groups to the carbonyl groups of Amadori compounds formed during the early stage of glycation and to 3-deoxyglucosone, a late-stage glycation product. This prevents further progression of the glycation reaction.
In vitro, aminoguanidine has been reported to suppress AGE formation and inhibit protein cross-linking and polymerization caused by glycation reactions.

What type of collagen is used in the neutralized collagen solution included in Collagen Glycation Assay Kit, Glucose/Fructose (Cat. No. PMC-AK70) and Collagen Glycation Assay Kit, Glyceraldehyde (Cat. No. PMC-AK71)?
The collagen is derived from porcine skin and is considered to contain predominantly type I collagen.

Can samples containing DMSO be used with Collagen Glycation Assay Kit, Glucose/Fructose (Cat. No. PMC-AK70) and Collagen Glycation Assay Kit, Glyceraldehyde (Cat. No. PMC-AK71)?
DMSO concentrations of 0.2% or less in the sample solution do not affect the assay. If higher concentrations are used, prepare a DMSO-only group and use it as the control.

Does the pH of the sample affect measurements in Collagen Glycation Assay Kit, Glucose/Fructose (Cat. No. PMC-AK70) and Collagen Glycation Assay Kit, Glyceraldehyde (Cat. No. PMC-AK71)?
Yes. The assay is affected by the pH of the sample. Please adjust samples using the buffer provided with the kit.
Particularly when strongly acidic samples are added, the cloudy collagen gel may dissolve and become transparent. If the gel dissolves, measurement results may be affected.

Can I use a CO2 incubator with Collagen Glycation Assay Kit, Glucose/Fructose (Cat. No. PMC-AK70) and Collagen Glycation Assay Kit, Glyceraldehyde (Cat. No. PMC-AK71)?
No. CO₂ incubator cannot be used because the buffer in the wells becomes acidic, which affects the glycation reaction.
Sealing the 96-well plate to block CO₂ is also not recommended because it restricts oxygen supply and inhibits the glycation reaction.
We recommend using a standard incubator. To maintain humidity, place the plate in a covered container together with moistened laboratory wipes and incubate under those conditions.

What are the differences between Collagen Glycation Assay Kit, Glucose/Fructose (Cat. No. PMC-AK70) and Collagen Glycation Assay Kit, Glyceraldehyde (Cat. No. PMC-AK71)?
Collagen Glycation Assay Kit, Glucose/Fructose (Cat. No. PMC-AK70):
• Uses glucose and fructose to initiate glycation.
• Suitable for evaluating early-stage glycation reactions induced by fructose.
• Supports long-term studies of approximately 10 to 30 days.

Collagen Glycation Assay Kit, Glyceraldehyde (Cat. No. PMC-AK71):
• Uses glyceraldehyde, a glucose metabolite, to initiate glycation.
• Provides results after a short glycation reaction period of approximately 24 hours.