Collagen AGEs Assay Kit, CMA-Specific, Glyoxal

Cosmo Bio LTD

Catalog No: CSR-AAS-AGE-K03

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Although carbohydrates are indispensable for ATP production, excess amounts of carbohydrates modify amino residues of amino acids such as lysine and arginine, and results in the irreversible functional disorders of proteins by changing the three-dimensional structure and net negative charge in patients with disordered metabolism. Since this reaction was first reported by Louis Camille Maillard in 1912, the reaction is called the Maillard reaction, or glycation. The Maillard reaction is divided by early and advanced stages. Early stage generates Amadori rearrangement products, such as haemoglobin A1c, whereas advanced stage generates the AGEs (advanced glycation end products), which is characterized by colour in brown and protein cross-linking. Collagen, the structural protein that forms skin, blood vessel wall and bone, also undergo glycation reaction.

N epsilon-carboxymethylarginine (CMA), an AGE component was identified in glycated collagen (1), it generates during the reaction of collagen with reducing sugars or glyoxal. AGEs accumulation in collagen induced dermal fibroblasts to undergo apoptosis (2). Because AGEs accumulate in collagen as a function of aging (3), CMA may be involved in aging of collagen-rich tissues such as skin. CML is detected in many proteins such as collagen and albumin, whereas CMA is generates specifically in collagen, suggesting that CMA may provide a marker for collagen glycation. An anti-CMA monoclonal antibody specificallyand sensitively detects CMA in collagen (4).

CML ELISA Assay Kit provides rapid detection of CMA formed by glycation with glyoxal on the collagen

coating plate. This kit is suitable to the research for functional foods and cosmetic materialswhich have anti-glycation activity.

Principle: Collagen coated on 96-well plate is glycated by glyoxal and CMA is formed onto collagen coating wells for 7days at 37 °C. The inhibitory effects of glycation by aminoguanidin (positive control) or samples are probed with an anti-CMA antibody, followed by an HRP conjugated secondary antibody. The inhibitory effect of glycation in unknown sample is determined by comparing with the inhibitory effect of glycation by aminoguanidin (positivecontrol).

“Collagen AGEs Assay Kit, CMA-Specific, Glyoxal” is suitable to the research for functional foods and cosmetic materials which have an anti-glycation activity.