Collagen Glycation Assay Kit, Glucose/Fructose

Cosmo Bio

Catalog No.: PMC-AK70

Product Description

For a long term examination that researches early saccharification from fructose. The non-enzymatic reaction of reducing carbohydrates with lysine side chains and N-terminal amino groups of macromolecules (proteins, phospholipids, and nucleic acids) is called the Maillard reaction or glycation. This reaction is initiated by the nonenzymatic reaction of reducing sugars with free amino groups on proteins to form Amadori products. The Amadori products undergo a variety of irreversible dehydration and rearrangement reactions, leading to the formation of advancedglycation end products (AGEs). AGEs have adverse effects on the functional properties of proteins. Many AGEs have fluorescent and covalent cross-linking properties. The accumulation of AGEs is thought to play an important role in the pathogenesis ofdiabetic patients and the aging process.

The collagen that forms bone, skin, and blood vessel is also glycated. 

Principle

The collagen gel formed in 96-well plates generates fluorescence after long-term incubation with glucose or fructose at 37°C. Some reagents or natural products inhibit this reaction. The Collagen Glycation Assay Kit, Glucose / Fructose is a complete assay system, which is designed to measure fluorescent AGEs using a fluorescence micro plate reader equipped with a 370 nm excitation filter and a 440 nm emission filter.

Collagen Glycation Assay Kit, Glucose/Fructose FAQs

What is the molecular weight of aminoguanidine included in Collagen Glycation Assay Kit, Glucose/Fructose (Cat. No. PMC-AK70) and Collagen Glycation Assay Kit, Glyceraldehyde (Cat. No. PMC-AK71)? Is it supplied as a hydrochloride salt?
The molecular weight of aminoguanidine is 110.55. It is supplied as the hydrochloride salt.

What is the mechanism by which aminoguanidine exhibits anti-glycation activity?
Aminoguanidine inhibits glycation by binding its amino groups to the carbonyl groups of Amadori compounds formed during the early stage of glycation and to 3-deoxyglucosone, a late-stage glycation product. This prevents further progression of the glycation reaction.
In vitro, aminoguanidine has been reported to suppress AGE formation and inhibit protein cross-linking and polymerization caused by glycation reactions.

What type of collagen is used in the neutralized collagen solution included in Collagen Glycation Assay Kit, Glucose/Fructose (Cat. No. PMC-AK70) and Collagen Glycation Assay Kit, Glyceraldehyde (Cat. No. PMC-AK71)?
The collagen is derived from porcine skin and is considered to contain predominantly type I collagen.

Can samples containing DMSO be used with Collagen Glycation Assay Kit, Glucose/Fructose (Cat. No. PMC-AK70) and Collagen Glycation Assay Kit, Glyceraldehyde (Cat. No. PMC-AK71)?
DMSO concentrations of 0.2% or less in the sample solution do not affect the assay. If higher concentrations are used, prepare a DMSO-only group and use it as the control.

Does the pH of the sample affect measurements in Collagen Glycation Assay Kit, Glucose/Fructose (Cat. No. PMC-AK70) and Collagen Glycation Assay Kit, Glyceraldehyde (Cat. No. PMC-AK71)?
Yes. The assay is affected by the pH of the sample. Please adjust samples using the buffer provided with the kit.
Particularly when strongly acidic samples are added, the cloudy collagen gel may dissolve and become transparent. If the gel dissolves, measurement results may be affected.

Can I use a CO2 incubator with Collagen Glycation Assay Kit, Glucose/Fructose (Cat. No. PMC-AK70) and Collagen Glycation Assay Kit, Glyceraldehyde (Cat. No. PMC-AK71)?
No. CO₂ incubator cannot be used because the buffer in the wells becomes acidic, which affects the glycation reaction.
Sealing the 96-well plate to block CO₂ is also not recommended because it restricts oxygen supply and inhibits the glycation reaction.
We recommend using a standard incubator. To maintain humidity, place the plate in a covered container together with moistened laboratory wipes and incubate under those conditions.

What are the differences between Collagen Glycation Assay Kit, Glucose/Fructose (Cat. No. PMC-AK70) and Collagen Glycation Assay Kit, Glyceraldehyde (Cat. No. PMC-AK71)?
Collagen Glycation Assay Kit, Glucose/Fructose (Cat. No. PMC-AK70):
• Uses glucose and fructose to initiate glycation.
• Suitable for evaluating early-stage glycation reactions induced by fructose.
• Supports long-term studies of approximately 10 to 30 days.

Collagen Glycation Assay Kit, Glyceraldehyde (Cat. No. PMC-AK71):
• Uses glyceraldehyde, a glucose metabolite, to initiate glycation.
• Provides results after a short glycation reaction period of approximately 24 hours.