Osteoclast cells (mouse)

Cosmo Bio

Catalog No.: PMC-OSC14C

Product Description

Bone marrow-derived osteoclast precursor cells from ICR mouse (frozen, 2.0 x 10⁶ cells/vial)

Citations for Osteoclast cells (mouse) – 4 Found

Egashira, Kenji; Ino, Yoko; Nakai, Yusuke; Ohira, Takashi; Akiyama, Tomoko; Moriyama, Kayano; Yamamoto, Yu; Kimura, Mitsuo; Ryo, Akihide; Saito, Tomoyuki; Inaba, Yutaka; Hirano, Hisashi; Kumagai, Ken; Kimura, Yayoi. Identification of gravity-responsive proteins in the femur of spaceflight mice using a quantitative proteomic approach. Journal Of Proteomics. 2023;288( 37482271):104976. 

Yamamichi, Gaku; Kato, Taigo; Arakawa, Noriaki; Ino, Yoko; Ujike, Takeshi; Nakano, Kosuke; Koh, Yoko; Motoyama, Yuichi; Outani, Hidetatsu; Myoba, Shohei; Ishizuya, Yu; Yamamoto, Yoshiyuki; Hatano, Koji; Kawashima, Atsunari; Fukuhara, Shinichiro; Uemura, Hiroji; Okada, Seiji; Morii, Eiichi; Nonomura, Norio; Uemura, Motohide. GDF15 propeptide promotes bone metastasis of castration-resistant prostate cancer by augmenting the bone microenvironment. Biomarker Research. 2024;12(1):147. 

Nagasawa, Yosuke; Takei, Masami; Iwata, Mitsuhiro; Nagatsuka, Yasuko; Tsuzuki, Hiroshi; Imai, Kenichi; Imadome, Ken-Ichi; Fujiwara, Shigeyoshi; Kitamura, Noboru. Human osteoclastogenesis in Epstein-Barr virus-induced erosive arthritis in humanized NOD/Shi-scid/IL-2Rγnull mice. Plos One. 16(4):e0249340. 

Ogata, Kenichi; Matsumura, Mayu; Moriyama, Masafumi; Katagiri, Wataru; Hibi, Hideharu; Nakamura, Seiji. Cytokine Mixtures Mimicking Secretomes From Mesenchymal Stem Cells Improve Medication-Related Osteonecrosis of the Jaw in a Rat Model. Jbmr Plus. 2018;2(2):69-80. 

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Osteoclast cells (mouse) FAQs

What is the composition of Rat Osteoclast Culture Medium (Cat. No. PMC-OSCMR), Mouse Osteoclast Culture Medium (Cat. No. PMC-OSCMM) and Human Osteoclast Culture Medium (Cat. No. PMC-OSCMHB)?
These culture media consist of α-MEM, 10% FBS, M-CSF, RANKL and Penicillin-Streptomycin.

How can I observe pits on dentine slices when using Rat Osteoclast Cells (Cat. No. PMC-OSC12C), Mouse Osteoclast Cells (Cat. No. PMC-OSC14C) or Human Osteoclast Cells (Cat. No. PMC-OSC15C) in a pit formation assay?
After staining the samples with hematoxylin, you can observe the pits on dentine slices under a stereomicroscope.

How long was Human Osteoclast Cells (Cat. No. PMC-OSC15C) cultured to generate the pits shown in Figure 4 of the datasheet?
The pits on the ivory slice were formed after 2 weeks of culture.

Can I passage Rat Osteoclast Cells (Cat. No. PMC-OSC12C), Mouse Osteoclast Cells (Cat. No. PMC-OSC14C) or Human Osteoclast Cells (Cat. No. PMC-OSC15C)?
Generally, it is difficult to detach osteoclasts from culture dishes using trypsin treatment. Known methods for passaging mature osteoclasts include physically detaching them with a cell scraper or culturing them on collagen gel plates beforehand. However, please note that both methods generally result in low recovery rates for viable cells.

Can I use Rat Osteoclast Culture Medium (Cat. No. PMC-OSCMR), Mouse Osteoclast Culture Medium (Cat. No. PMC-OSCMM) and Human Osteoclast Culture Medium (Cat. No. PMC-OSCMHB) after refreezing?
If you plan to use these culture media in multiple experiments, aliquot them into 10 mL tubes after thawing and store the aliquots at -20°C or below. Repeated freeze-thaw cycles should be avoided because they may reduce the activity of M-CSF and RANKL. Once thawed, each aliquot should be used immediately.

How should I culture Rat Osteoclast Cells (Cat. No. PMC-OSC12C), Mouse Osteoclast Cells (Cat. No. PMC-OSC14C) or Human Osteoclast Cells (Cat. No. PMC-OSC15C) to prevent them from differentiating into osteoclasts?
We recommend using these culture media without RANKL. To avoid culture variations caused by different serum lots, please use Osteoclast Wash Medium (Cat. No. PMC-OSCMW). Also, you should add M-CSF to the washing medium.

How many days does it take for Rat Osteoclast Cells (Cat. No. PMC-OSC12C), Mouse Osteoclast Cells (Cat. No. PMC-OSC14C) and Human Osteoclast Cells (Cat. No. PMC-OSC15C) to form osteoclasts, and how long can they be maintained in culture?
You can observe osteoclast formation after the following culture periods:

Rat Osteoclast Cells / Mouse Osteoclast Cells:
Osteoclast formation is typically observed after 4–5 days of culture. Cells can be maintained for 2 weeks or longer.

Human Osteoclast Cells:
Osteoclast formation is typically observed after 4–10 days of culture. Cells can be maintained for 2 weeks or longer.