Atelocollagen Honeycomb sponge

KOKEN

Catalog No.:
KOU-CSH-10
Atelocollagen Honeycomb sponge
Atelocollagen Honeycomb sponge
Atelocollagen Honeycomb sponge
Atelocollagen Honeycomb sponge
Suppression of teratoma formation by embryoid body (EB) following transplantation of EB on Honeycomb Sponge (CSH) (Yamaki M, Harvard University, Stem Cell Regenerative Biology) Mouse kidney at 12 weeks following transplantation of EB on CSH scaffold (EB+CSH) barely showed sign of teratoma formation while EB transplanted without CSH (EB alone) generated teratomas in all mice. Histologically, transplanted EB+CSH were indistinguishable from adjacent host renal tissue, suggesting spontaneous differentiation without specific induction. Methods: Plate cultured mouse embryonic stem (ES) cells were trypsinized, filtered through nylon mesh, seeded into 96-well plates (1x104 cells/well), and cultured for 5 days to form embryoid bodies. When EB were mixed with CSH, They were rapidly and uniformly integrated into the CSH matrix. EB+CSH complex was then transplanted under renal capsule of 6-week-old mice (Ref. 7).
3D culture of vascular smooth muscle cells (SMCs) using CSH (Ishii I, Graduate School of Pharmaceutical Sciences, Chiba University) It has been reported that mouse SMCs cultured in CSH turn to differentiated state from dedifferentiated state and also the proliferation is suppressed (Ref. 6). In order to investigate the mechanism of proliferative inhibition, p27 deficient mouse derived SMCs and p21Cip1 siRNA were used but the effect on proliferation was observed only in SMCs cultured on plates. Although further research is required to clarify the mechanism, SMCs cultured in CSH can maintain similar features to in vivo aortic SMCs for more than one month and therefore it may be useful for regenerative medicine (Ref. 1).
Administration of medical plant extract and cell migration using CSH (Maeda A, Ph. D. Skin Regeneration, PIAS Collaborative Research, Osaka University) he ethanol extract from Mallotus philippinensis bark (EMPB) is impregnated to CSH and topically applied to the wound of mouse. Then, luciferase-expressing MSCs (Luc-MSCs) were intravenously injected. As a result, accumulation of Luc-MSCs at the wound area was promoted in EMPB treated group. Further evaluations at 17 days after EMPB administration to the wound area revealed that accelerated wound healing and increased numbers of capillaries and granulation tissue. These results suggests that CSH is useful for sustained release of bioactive substance (Ref. 5).
Implantation of bone marrow stem cells (BMSCs) into hemisected spinal cord using CSH (Enomoto M, Department of Orthopaedic Surgery and Hyperbaric Medical Center, Tokyo Medical and Dental University) A previous report demonstrated that the pore structure of CSH enhances nerve regeneration (Ref. 9). In this research, a remarkable increase of neurite growth was observed when dorsal root ganglia (DRG)s were cultured on GEP-expressing BMSCs contained CSH (BMSCs+CSH) for ten days compared to DRGs cultured on CSH alone. Improved locomotor and sensory function was also observed four weeks after implantation of BMSC+CSH into hemisected spinal cord (Ref. 4). [Tuj-1: A maker for neuron; SMI31: A marker for neurofilament; GFAP: a marker for astrocyte; BBB score: an evaluation method for hindlimb motor function]
Induction of hepatic differentiation of ES cells and transplantation using CSH Embryoid bodies (EBs) were formed from ES cells and then inserted into CSH. EBs both with and without CSH were cultured to differentiate and induce hepatic histogenesis. The EB-derived cells expressed liver-specific genes, and albumin-positive cells formed cordlike structures that were not present in those without CSH. The scaffold including EB-derived hepatocyte-like cells was transplanted into the median lobe of mice. After 14 days, cells positive for both albumin and cytokeratin 18 appeared in the transplant and formed clustered aggregates. (Ref. 10) [AFP: Marker for inicial livers hepatic cells; ALB: Marker for inicial to matured hepatic cells; G6P and CK18: Marker for matured hepatic cells]
Atelocollagen Honeycomb sponge
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Background

Honeycomb Sponge is made from type I atelocollagen and possess honeycomb-like pore structure with high pore density. This honeycomb structure enables easy supply of nutrients to cells and excretion of waste products from cells.

Applications

  • 3D cell culture
  • Scaffold for regenerative medicine research
  • Sustained release of bioactive substances

Features

  • Unidirectional pore structure facilitates cell migration and vascularization in vivo
  • Cells can be easily harvested by collagenase treatment.

This product is manufactured in Japan and produced from raw materials obtained from livestock certified born and raised in Australia or New Zealand. Japan, Australia and New Zealand are all recognized by the World Health Organization as having negligible risk for Bovine Spongiform Encephalitis (BSE). Nevertheless, please consult with your country's Customs office to confirm importability.




Documents & Links for Atelocollagen Honeycomb sponge
Datasheet Atelocollagen Honeycomb sponge Datasheet
Flyer Atelocollagen Honeycomb sponge Flyer
Purchase Agreement Direct Shipment and Fees Authorization

Documents & Links for Atelocollagen Honeycomb sponge
Datasheet Atelocollagen Honeycomb sponge Datasheet
Flyer Atelocollagen Honeycomb sponge Flyer
Purchase Agreement Direct Shipment and Fees Authorization
Citations for Atelocollagen Honeycomb sponge – 17 Found
Hayashi, Koichiro; Tokumaru, Tatsuya; Shibahara, Keigo; Taleb Alashkar, Ahmad Nazir; Zhang, Cheng; Kishida, Ryo; Nakashima, Yasuharu; Ishikawa, Kunio. Wood-Derived Hydrogels for Osteochondral Defect Repair. Acs Nano. 2025;19(1):520-534.  PubMed
- Comments: Bone, Tooth, Cartilage
Kishikawa M, Ueda M, Yasui K, Morikuni H, Hashimoto Y, Nishiura A.. Bone regeneration using three-dimensional spheroids derived from dedifferentiated fat cells. Oral Sci Int.. 2025;22(1):e1286. 
- Comments: Bone, Tooth, Cartilage
Cho, Yung-Chieh; Peng, Pei-Wen; Ou, Yu-Sin; Liu, Chung-Ming; Huang, Bai-Hung; Lan, Wen-Chien; Kuo, Hsin-Hui; Hsieh, Chia-Chien; Chen, Brian; Huang, Mao-Suan; Nakano, Hiroyuki. An Innovative Design to Enhance Osteoinductive Efficacy and Biomechanical Behavior of a Titanium Dental Implant. Materials (Basel, Switzerland). 2024;17(10)  PubMed
- Comments: Bone, Tooth, Cartilage
Liu, Shuang. Scaffolded Chondrogenic Spheroid-Engrafted Model. Methods In Molecular Biology (Clifton, N.j.). 2766:17-24.  PubMed
- Comments: Bone, Tooth, Cartilage
Kim, Byumsu; Bouklas, Nikolaos; Cohen, Itai; Bonassar, Lawrence J. Instabilities induced by mechanical loading determine the viability of chondrocytes grown on porous scaffolds. Journal Of Biomechanics. 2023;152:111591.  PubMed
- Comments: Bone, Tooth, Cartilage
Adachi, Tetsuya; Miyamoto, Nao; Imamura, Hayata; Yamamoto, Toshiro; Marin, Elia; Zhu, Wenliang; Kobara, Miyuki; Sowa, Yoshihiro; Tahara, Yoshiro; Kanamura, Narisato; Akiyoshi, Kazunari; Mazda, Osam; Nishimura, Ichiro; Pezzotti, Giuseppe. Three-Dimensional Culture of Cartilage Tissue on Nanogel-Cross-Linked Porous Freeze-Dried Gel Scaffold for Regenerative Cartilage Therapy: A Vibrational Spectroscopy Evaluation. International Journal Of Molecular Sciences. 2022;23(15)  PubMed
- Comments: Bone, Tooth, Cartilage
Kaneko, Hiroshi; Hasegawa, Daigaku; Itoyama, Tomohiro; Yoshida, Shinichiro; Tomokiyo, Atsushi; Hamano, Sayuri; Sugii, Hideki; Maeda, Hidefumi. Inhibition of c-Jun N-terminal kinase signaling promotes osteoblastic differentiation of periodontal ligament stem cells and induces regeneration of periodontal tissues. Archives Of Oral Biology. 2022;134:105323.  PubMed
- Comments: Bone, Tooth, Cartilage
Kato, Hiroshi; Watanabe, Katsuhito; Saito, Akiko; Onodera, Shoko; Azuma, Toshifumi; Takano, Masayuki. Bone regeneration of induced pluripotent stem cells derived from peripheral blood cells in collagen sponge scaffolds. Journal Of Applied Oral Science : Revista Fob. 30:e20210491.  PubMed
- Comments: Bone, Tooth, Cartilage
Kim, B., Middendorf, J.M., Diamantides, N. et al.. The Role of Buckling Instabilities in the Global and Local Mechanical Response in Porous Collagen Scaffolds. Exp Mech. 2022;62:1067–1077. 
- Comments: Bone, Tooth, Cartilage
Okamoto, Keiichiro; Kakihara, Yoshito; Ohkura, Naoto; Tohma, Aiko; Washio, Ayako; Kitamura, Chiaki; Noiri, Yuichiro; Yamamura, Kensuke; Saeki, Makio. Effects of rice fermented extracts, "Sake Lees", on the functional activity of odontoblast-like cells (KN-3 cells). Odontology. 2022;110(2):254-261.  PubMed
- Comments: Bone, Tooth, Cartilage
Kusanagi, Akihiko; Blahut, Eric B; Ogura, Takahiro; Tsuchiya, Akihiro; Mizuno, Shuichi. Repairing Cartilage with Processed Chondrocyte Constructs: A 6-Month Study Using a Porcine Model. Cartilage. 2021;13(2_suppl):1088S-1101S.  PubMed
- Comments: Bone, Tooth, Cartilage
Middendorf, Jill M; Diamantides, Nicole; Kim, Byumsu; Dugopolski, Caroline; Kennedy, Stephen; Blahut, Eric; Cohen, Itai; Bonassar, Lawrence J. The influence of chondrocyte source on the manufacturing reproducibility of human tissue engineered cartilage. Acta Biomaterialia. 2021;131:276-285.  PubMed
- Comments: Bone, Tooth, Cartilage
Chang, Xuboya; Tamauchi, Satoshi; Yoshida, Kosuke; Yoshihara, Masato; Yokoi, Akira; Shimizu, Yusuke; Ikeda, Yoshiki; Yoshikawa, Nobuhisa; Kiyono, Tohru; Yamamoto, Yusuke; Kajiyama, Hiroaki. Downregulating vaccinia-related kinase 1 by luteolin suppresses ovarian cancer cell proliferation by activating the p53 signaling pathway. Gynecologic Oncology. 2023;173:31-40.  PubMed
Mogi, Kazumasa; Koya, Yoshihiro; Yoshihara, Masato; Sugiyama, Mai; Miki, Rika; Miyamoto, Emiri; Fujimoto, Hiroki; Kitami, Kazuhisa; Iyoshi, Shohei; Tano, Sho; Uno, Kaname; Tamauchi, Satoshi; Yokoi, Akira; Shimizu, Yusuke; Ikeda, Yoshiki; Yoshikawa, Nobuhisa; Niimi, Kaoru; Yamakita, Yoshihiko; Tomita, Hiroyuki; Shibata, Kiyosumi; Nawa, Akihiro; Tomoda, Yutaka; Kajiyama, Hiroaki. 9-oxo-ODAs suppresses the proliferation of human cervical cancer cells through the inhibition of CDKs and HPV oncoproteins. Scientific Reports. 2023;13(1):19208.  PubMed
Yuki Nishikawa, Atsuko Niwa, Kazuko Sakai, Kazuto Nishio, Noritaka Isogai. Human Adipose-derived Stem Cells Induce Cell Senescence in a Keloid Model. Medical Journal of Kindai University / formerly Acta Medica Kinki University. 2022;47(2):27–38. 
Brizi, Valerio; Buttò, Sara; Cerullo, Domenico; Michele Lavecchia, Angelo; Rodrigues-Diez, Raquel; Novelli, Rubina; Corna, Daniela; Benigni, Ariela; Remuzzi, Giuseppe; Xinaris, Christodoulos. Human iPSC-derived neural crest stem cells can produce EPO and induce erythropoiesis in anemic mice. Stem Cell Research. 2021;55:102476.  PubMed
- Comments: Subcutaneous transplantation of neural crest cells seeded on a honeycomb scaffold into mice
Morinaga, Hironobu; Mohri, Yasuaki; Grachtchouk, Marina; Asakawa, Kyosuke; Matsumura, Hiroyuki; Oshima, Motohiko; Takayama, Naoya; Kato, Tomoki; Nishimori, Yuriko; Sorimachi, Yuriko; Takubo, Keiyo; Suganami, Takayoshi; Iwama, Atsushi; Iwakura, Yoichiro; Dlugosz, Andrzej A; Nishimura, Emi K. Obesity accelerates hair thinning by stem cell-centric converging mechanisms. Nature. 2021;595(7866):266-271.  PubMed


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Import Permit Required for US Customers
This product contains animal-derived, plant-derived, or agricultural materials, such as bovine, porcine, equine, etc., components. These are regulated materials and permits ensure they are imported into the United States in compliance with USDA requirements.
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Atelocollagen Honeycomb sponge FAQs

What percentage of cells attach onto Atelocollagen Honeycomb sponge (Cat. No. KOU-CSH-10) during culture?
Approximately 20-30% of seeded cells attach onto Atelocollagen Honeycomb products, although the type of cell and the seeding method may affect attachment.

How can Atelocollagen Honeycomb sponge (Cat. No. KOU-CSH-10) be used?
Some applications include implanting a sponge impregnated with a specific protein to induce nerve cells into the sponge, as well as three-dimensional culture of vascular smooth muscle cells within the sponge. Additional examples with various cell types are available (Please see the list of publications for details).

Has Atelocollagen Honeycomb sponge (Cat. No. KOU-CSH-10) been used to culture iPSCs?
Atelocollagen Honeycomb products have been used in the differentiation and transplantation of iPSC-derived neural stem cells.

How long does Atelocollagen Honeycomb sponge (Cat. No. KOU-CSH-10) remain at the transplantation site?
We estimate that it remains for approximately one month; however, the duration depends on the transplantation site.

Is it possible to obtain custom-made Honeycomb sponges that are different in shape and pore size from the off-the-shelf product?
Please contact us, as custom-made products may be available upon request.
You can also cut Atelocollagen Honeycomb products into smaller pieces with a sharp knife such as a surgical scalpel.

How can I seed cells into Atelocollagen Honeycomb sponge (Cat. No. KOU-CSH-10)?
Please refer to the product manual, Atelocollagen Honeycomb sponge Datasheet.

How can I harvest cells from Atelocollagen Honeycomb sponge (Cat. No. KOU-CSH-10)?
Add collagenase to a final concentration of 0.1% and incubate at 37°C for approximately 30 minutes to 1 hour.

Is it possible to prepare sections from Atelocollagen Honeycomb sponge (Cat. No. KOU-CSH-10)?
Similar to tissue samples, Atelocollagen Honeycomb products can be fixed and subsequently embedded in paraffin or OCT compound for section preparation.

What precautions should I take when using collagen products for staining?
Collagen itself has blue autofluorescence. Therefore, fluorescent dyes in the yellow-to-red wavelength range are recommended to distinguish immunostains.
For example, Lipi-Red and DRAQ5 can be used to stain adipocytes and nuclei, respectively.
In H&E staining, collagen is stained reddish purple by eosin. Because it stains darker than the cytoplasm, consider Masson trichrome staining if you would like to distinguish cells from collagen.

Which atelocollagen products are used for cell transplantation?
If the transplantation site can be incised, transplantation of an atelocollagen sponge or membrane-containing cells is applicable.

Atelocollagen products:
3D Honeycomb Boosted (Cat. No. KOU-3D-HCB)
Collagen Sponge Honeycomb (Cat. No. KOU-CSH-10)
Honeycomb Disk 96 (Cat. No. KOU-CSH-96)
Collagen Sponge Mighty (Cat. No. KOU-CSM-25)
FibColl® Atelocollagen Inserts (Cat. No. KOU-FAI-24)
Atelocollagen Permeable Membrane (Cat. No. KOU-CM-24 and Cat. No. KOU-CLF-01)

If the incision is less preferable, it is possible to mix cells with a high-concentration of atelocollagen solution and administer it locally so that the collagen forms a gel and cells can be transplanted to the injection site.
Product to prepare a high-concentration atelocollagen solution: Atelocollagen Powder (Cat. No. KOU-CLP-01)

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