Atelocollagen sponge Mighty

KOKEN

Catalog No.:
KOU-CSM-25
Cross-sectional image of MIGHTY seeded with Mouse Fibroblast cell (NIH3T3, fluorescent microscopy)
Sectional view of MIGHTY 1 month after transplantation under the skin of a mouse. Penetration of the cells and maintenance of the MIGHTY sponge structure were confirmed.
Atelocollagen sponge Mighty
Cell proliferation using MIGHTY
Compressive loading culture experiment (Shimomura K, et al. Osaka University) Human synovium derived cells were cultured on MIGHTY (Fig. 1). After 3 days of culture, cyclic compressive loading of 40 kPa was applied to the sponge for 1 hour and then expression levels of arthritis related genes PGE2, IL-6 and IL-8 were measured and found elevated. On the other hand, expression levels of proinflammatory cytokines, IL-1 β and TNF- α , were unchanged (data not shown), which suggests there might be another signal pathway regulating PGE2 expression. mRNA level of mPGES-1 (PGE2 synthase) were upregulated by mechanical stress and this upregulation was suppressed by COX-2 selective inhibitor in a dose-dependent manner (Fig. 2).
Compressive loading culture experiment (Shimomura K, et al. Osaka University) With dexamethasone, up-regulated protein expression of PGE2, IL-6 and IL-8 were suppressed in a dose- dependent manner. These results show usefulness for MIGHTY in an in vitro arthritis model (Ref. 8).
Atelocollagen sponge Mighty
Atelocollagen sponge Mighty
SEM image of cross section through MIGHTY
Atelocollagen sponge Mighty
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Background
Atelocollagen sponge MIGHTY consists primarily of type I atelocollagen derived from bovine dermis and is strong enough to withstand compressive loading of up to 40 kPa. Culturing cells in MIGHTY under cyclic compressive loading provides a more in vivo-like environment to evaluate cell function. MIGHTY is also applicable as a scaffold for conventional 3D cell culture.

Applications

  • Cell culture under mechanical stimuli
  • Conventional 3D cell culture
  • Scaffold for regenerative medicine research

Features

  • Retains structural rigidity during repeated compression loading of 40 kPa.
  • Sized for 96-well plates.
  • Made of highly purified type I atelocollagen derived from bovine dermis.

This product is manufactured in Japan and produced from raw materials obtained from livestock certified born and raised in Australia or New Zealand. Japan, Australia and New Zealand are all recognized by the World Health Organization as having negligible risk for Bovine Spongiform Encephalitis (BSE). Nevertheless, please consult with your country's Customs office to confirm importability.




Documents & Links for Atelocollagen sponge Mighty
Datasheet Atelocollagen sponge Mighty Datasheet
Flyer Atelocollagen sponge Mighty Flyer
Purchase Agreement Direct Shipment and Fees Authorization

Documents & Links for Atelocollagen sponge Mighty
Datasheet Atelocollagen sponge Mighty Datasheet
Flyer Atelocollagen sponge Mighty Flyer
Purchase Agreement Direct Shipment and Fees Authorization


Documentation Requirements for Order Placement

Import Permit Required for US Customers
This product contains animal-derived, plant-derived, or agricultural materials, such as bovine, porcine, equine, etc., components. These are regulated materials and permits ensure they are imported into the United States in compliance with USDA requirements.
Prior to placing an order, customers are required to apply for a VS 16-3 Import Permit directly from the US government regulatory agency, APHIS (Animal and Plant Health Inspection Service). Please click here for full guidance on applying for an import permit.
Any questions? We are ready to assist you in the process. Please contact us at support@cosmobiousa.com or call us at +1 760 431 4600

Direct Shipment and Fees Authorization
This item must ship directly from our Japan office due to permit requirements, temperature sensitivity, or other shipping considerations. Access the direct shipment authorization form in the Documents section above. Please download and fill in the document as requested. Submit the completed document with your order to orders@cosmobiousa.com.

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