Atelocollagen Permeable Membrane Sheet

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Catalog No.:: KOU-CLF-01

Remodeling epithelial polarity using the permeable collagen membrane (Yoshizato K, et al. J Cell Sci. 91(Pt4): 491-499)

Glucose consumption and lactic acid excretion in the basal compartment was significantly higher than that of the apical compartment. On the other hand, pH reduction in the apical compartment was abolished by membrane transporter inhibitor.

Induction of urinary tract differentiation from embryo body (EB) and transplantation with the permeable collagen membrane. (Kinebuchi Y, et al., Shinonoi General Hospital/Shinshu Univrsity, Japan.)

Following 28 days culture of mouse EB on a collagen membrane using KSFM medium and 3T3 cell culture supernatant, a four layer structure was formed. A duct-like structure was observed when cultured tissue attached to collagen membrane was transplanted into mice. Expression of UPIII (a marker of urothelium) and CK8 and CK18 (markers of urinary bladder) were also observed. These results suggest that collagen membranes are useful for the formation of tissue like structures and may also be useful following transplantation.

Induction of corneal endothelial differentiation and transplantation with the permeable collagen membrane (Hato S, et al., Keio University, Japan.)

ultipotent Cornea-derived precursors isolated from mouse and human corneal stroma were cultured on collagen membrane and differentiated to functional corneal endothelium. Transplanting TECE attached collagen membrane to donor cornea where corneal endothelium was stripped suppressed edematous and notably increased the transparency of cornea compared to control groups. These results show that collagen membrane, with its high transparency and biocompatibility, is suitable for transplantation, such as corneal transplantation.

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Background
Permeable collagen membranes are made from highly purified bovine dermal type I atelocollagen for single and double layer cell culture applications. These membranes are particularly suitable for studying cell-cell interactions between two different cell types seeded on opposite sides of the same membrane. Small molecules pass freely through the membrane while cells cannot. They are also useful for remodeling epithelial polarity. When epithelial cells are cultured on the membrane, cells will absorb nutrients and excrete metabolic waste through the membrane.

Applications

in vitro experiments: cell-cell interaction studies; remodeling of epithelial polarity; primary cell culture
in vivo experiment: cell transplantation

This product is manufactured in Japan and produced from raw materials obtained from livestock certified born and raised in Australia or New Zealand. Japan, Australia and New Zealand are all recognized by the World Health Organization as having negligible risk for Bovine Spongiform Encephalitis (BSE). Nevertheless, please consult with your country's Customs office to confirm importability.

Documents & Links for Atelocollagen Permeable Membrane Sheet
Datasheet	Atelocollagen Permeable Membrane Sheet Datasheet
Flyer	Atelocollagen Permeable Membrane Sheet Flyer
Flyer	Atelocollagen Permeable Membrane Sheet Flyer
Purchase Agreement	Direct Shipment and Fees Authorization

Documents & Links for Atelocollagen Permeable Membrane Sheet
Datasheet	Atelocollagen Permeable Membrane Sheet Datasheet
Flyer	Atelocollagen Permeable Membrane Sheet Flyer
Flyer	Atelocollagen Permeable Membrane Sheet Flyer
Purchase Agreement	Direct Shipment and Fees Authorization

Documentation Requirements for Order Placement

Import Permit Required for US Customers
This product contains animal-derived, plant-derived, or agricultural materials, such as bovine, porcine, equine, etc., components. These are regulated materials and permits ensure they are imported into the United States in compliance with USDA requirements.
Prior to placing an order, customers are required to apply for a VS 16-3 Import Permit directly from the US government regulatory agency, APHIS (Animal and Plant Health Inspection Service). Please click here for full guidance on applying for an import permit.
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Direct Shipment and Fees Authorization
This item must ship directly from our Japan office due to permit requirements, temperature sensitivity, or other shipping considerations. Access the direct shipment authorization form in the Documents section above. Please download and fill in the document as requested. Submit the completed document with your order to orders@cosmobiousa.com.