Yatalase, Fungal Cell Lytic Enzymes

Cosmo Bio LTD

Catalog No: OZK-OZ-10EX

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Yatalase is used to lyse cell walls of filamentous fungi. The product is prepared from culture supernatants of Corynebacterium sp. OZ-21, and consists mainly of chitinase, chitobiase and β-1,3-glucanase.

Features
Has excellent thermostability and can be stored at room temperature. Efficiently digests native chitin. Has the revitalization of Chitinase, Chitobiase, Chitosanase, and β-1,3-Glucanarse Can be used alone to prepare protoplasts from filamentous fungi.

Source
Corynebacterium sp. OZ-21 Form : Lyophilized powder(containing lactose)

Storage
Lyophilized preparation is stable for at least 1 year at 4°C.
Maintain at -20°C for long term strage. Avoid freeze/thaw cycles.

Specific activities
Chitinase activity : Approximately 50 units/g powder
Chitobiase activity : Approximately 500 units/g powder Lytic activity against cell walls : Approximately 10,000 units/g powder

Properties
Optimum pH : pH 5〜8 Optimum temperature for enzyme activity : 30〜50℃

UNIT DEFINITION
Chitinase activity: One unit of chitinase activity is determined as the amount required to release 1µmol of N-acetylglucosamine from chitin in 1 minute.
Chitobiase activity: One unit of chitobiase activity is defined as the amount of enzyme required to release 1µmol of p-nitrophenol from p-nitrophenyl-N-acetyl-β-D-glucosaminide in 1 minute. Lytic activity: One unit of enzyme activity is defined as the amount required to cause a 1% decrease in absorbance in 1 hour.