Yatalase, Fungal Cell Lytic Enzymes

Ozeki

Catalog No.: OZK-OZ-10EX

Background

Yatalase is used to lyse cell walls of filamentous fungi. The product is prepared from culture supernatants of Corynebacterium sp. OZ-21, and consists mainly of chitinase, chitobiase and β-1,3-glucanase.

Features

Has excellent thermostability and can be stored at room temperature. Efficiently digests native chitin. Has the revitalization of Chitinase, Chitobiase, Chitosanase, and β-1,3-Glucanarse Can be used alone to prepare protoplasts from filamentous fungi.

Source

Corynebacterium sp. OZ-21 Form : Lyophilized powder(containing lactose)

Storage

Lyophilized preparation is stable for at least 1 year at 4°C.
Maintain at -20°C for long term strage. Avoid freeze/thaw cycles.

Specific activities 

Chitinase activity : Approximately 50 units/g powder
Chitobiase activity : Approximately 500 units/g powder Lytic activity against cell walls : Approximately 10,000 units/g powder

Properties 

Optimum pH : pH 5〜8 Optimum temperature for enzyme activity : 30〜50℃

Unit definition

Chitinase activity: One unit of chitinase activity is determined as the amount required to release 1µmol of N-acetylglucosamine from chitin in 1 minute.
Chitobiase activity: One unit of chitobiase activity is defined as the amount of enzyme required to release 1µmol of p-nitrophenol from p-nitrophenyl-N-acetyl-β-D-glucosaminide in 1 minute. Lytic activity: One unit of enzyme activity is defined as the amount required to cause a 1% decrease in absorbance in 1 hour.

Citations for Yatalase, Fungal Cell Lytic Enzymes – 0 Found

Reissig, J. L., Strominger, J. L. and Leloir, L. F.. J. Biol. Chem.. 1955;217:959. 

Fujii, F., Ozeki, K., Kanda, A., Hamaji, M., and Nunokawa, Y.. 1998;87:757. 

Ozeki, K., Ishimaru (Nagamasu), Y., Koda, A., Minetoki, T., Obuchi, K., Hamaji, M., and Kumagai, C. . 1998;76:187. 

Yatalase, Fungal Cell Lytic Enzymes FAQs

Is Yatalase (Cat. No. OZK-OZ-10EX) effective against any type of filamentous fungus?
The following filamentous fungi can be used to prepare protoplasts very efficiently with reduced enzyme usage:

• Aspergillus oryzae
• Trichoderma reesei
• T. viride
• T. koningii

For other filamentous fungi, including Aspergillus species, Ascomycetes, Zygomycetes, and Basidiomycetes, if protoplasts are difficult to obtain, we recommend using Yatalase in combination with 0.5% Cellulase "Onozuka" R-10.

Are there any other enzymes available for lysing microbial cell walls besides Yatalase (Cat. No. OZK-OZ-10EX)?
Yes. We offer cell wall lytic enzymes for different types of microorganisms.Please select the enzyme according to the target microorganism.

・Labiase Bacterial Cell Lytic Enzymes (Cat. No. OZK-OZ-30EX): Suitable for bacterial cell lysis, including lactic acid bacteria.
・Yatalase™ Fungal Cell Lytic Enzymes (Cat. No. OZK-OZ-10EX): Suitable for fungal cell lysis.
・Westase™ Yeast Cell Lytic Enzymes (Cat. No. OZK-OZ-20EX): Suitable for yeast cell lysis.