Westase Yeast Cell Lytic Enzymes

Ozeki

Catalog No.: OZK-OZ-20EX

Application:

Digestive enzyme of cell walls of yeast Westase was prepared from liquid culture supernatant of Streptomyces rochei DB-34. This product has complex lytic activities of yeast cell mainly consisting of (beta)-1,6 glucanase and (beta)-1,3 glucanase activity. This enzyme works well forAscosporogenous yeasts such as Saccharomyces cerevisiae. But it is also available for fission yeasts such as Schizosaccharomyces pombe which cannot be protoplasted just with Zymolyase treatment or Basidiosporogenous yeasts, Imperfect yeasts such as Ustilago maydis, Phaffia rhodozyma, Cryptococcus albidus, etc. which are rarely protoplasted, and make them efficiently into protoplast.

Definition of Activity:

(beta)-1,6 glucanase activity
One unit is defined as the amount required to release 1 (mu)mol reducing sugar from 10 mg/ml Pustulan solution in 1 min. at 37°C, pH6.0.

Lytic activity:

One unit is defined as the amount required to cause a 1% decrease in absorbance at 660 nm in 1 min. at 30°C, pH6.0 when using cell wall fraction of Cryptococcus albidus IFO 0612

Specific activities:

(beta)-1,6 glucanase activity : >400 units / g

Lytic activity :

>35,000 units / g
DNase activity : ND (McIlvain Buffer, pH6.0)

Storage:

Lyophilized preparation is stable for at least 1 year when stored at 4°C.

Westase Yeast Cell Lytic Enzymes FAQs

Is filtration required when using Westase Yeast Cell Lytic Enzymes (Cat. No. OZK-OZ-20EX)?
Yes. The diatomaceous earth contained as an excipient in Westase Yeast Cell Lytic Enzymes is insoluble, so without filtration, a precipitate will form and the product will be difficult to handle.
Please be sure to perform filtration. Diatomaceous earth also acts as a filter aid.

Does the buffer have to be McIlvaine when using Westase Yeast Cell Lytic Enzymes (Cat. No. OZK-OZ-20EX)?
No. McIlvaine buffer is not required. Please use a buffer that is suitable for your application.

What is the appropriate concentration of Westase Yeast Cell Lytic Enzymes (Cat. No. OZK-OZ-20EX) to use?
A concentration of 0.5% during the reaction is expected to be sufficient, but depending on the application, a lower concentration may be preferable.

I was unable to produce protoplasts when using sorbitol as an osmotic adjuster with Westase Yeast Cell Lytic Enzymes (Cat. No. OZK-OZ-20EX). What should I do?
Westase Yeast Cell Lytic Enzymes cannot exert its full effect unless an organic acid salt is used as an osmotic pressure adjuster.
Be sure to use an organic acid salt, preferably sodium tartrate. When regenerating protoplasts for transformation, use sorbitol as an osmotic agent.

Are there any other enzymes available for lysing microbial cell walls besides Westase Yeast Cell Lytic Enzymes (Cat. No. OZK-OZ-20EX)?
Yes. We offer cell wall lytic enzymes for different types of microorganisms.

Labiase Bacterial Cell Lytic Enzymes (Cat. No. OZK-OZ-30EX): Suitable for bacterial cell lysis, including lactic acid bacteria.
Yatalase™ Fungal Cell Lytic Enzymes (Cat. No. OZK-OZ-10EX): Suitable for fungal cell lysis.
Westase™ Yeast Cell Lytic Enzymes (Cat. No. OZK-OZ-20EX): Suitable for yeast cell lysis.

Please select the enzyme according to the target microorganism.