PrimeSurface Cultureware White Plate

S-BIO

Catalog No.: SBC-MS-9096WZ

Product Description

PrimeSurface White plates are optimized for chemiluminescent assays, which often outperform fluorescent assays due to their low background and high signal-to-noise ratio.
White plates reflect light to maximize signal output, enhancing assay sensitivity. PrimeSurface cultureware are ultra-low attachment (ULA) dishes and microplates pre-coated with a proprietary ultra-hydrophilic polymer that enables uniform, spontaneous spheroid formation. Typically, tumor spheroids are generated using ULA plates and then transferred to white plates for luminescence readout, which may damage spheroids. PrimeSurface White 96- and 384-well ULA plates allow for both spheroid formation and luminescence analysis within the same plate, streamlining workflows, reducing assay time, preventing spheroid damage, and providing robust, high-sensitivity luminescent HTS assays.

Application

• Stem Cell Research: Induction of ES, iPS and mesenchymal stem cells differentiation by embryoid body formation.
• Drug Screening Research & Development: An ideal product for drug response studies. 3D spheroid models are more physiologically relevant than 2D monolayer model.
• Compatible with brightfield and fluorescence Imaging and Confocal microscopy:
    •    NoviSight 3D High Content Analysis by Olympus
    •    Celigo® by Nexcelom
    •    Spheroid applications on IncuCyte® by Sartorius
    •    Cytation 5 Biotek
• Tissue engineering and Regenerative medicine

Technical Data

•    Generation of Human iPS Cell-Derived Kidney Organoids and Their Application in Drug Discovery Research
•    Stimulus effects Evaluation
•    Blood-brain barrier (BBB)
•    Comparison data (3D spheroid culture using HepG2 cell line)
•    For Tissue engineering and Regenerative medicine
•    Application example of hES cells differentiation using PrimeSurface
     The uniform spheroids form spontaneously within one day for effective aggregate formation of ES cells by using PrimeSurface

Please check the S-Bio’s website for more detail example of application.
Link: https://www.sumibe.co.jp/english/product/s-bio/cell-culture/primesurface-96u/index.html

Specifications

Cat. No.	Product	Wells	Bottom	Volume	Qty/Pk	Qty/Cs
SBC-MS-9096WZ	PrimeSurface 96 well U White Plate	96	U bottom	0.3mL	1	20
SBC-MS-9384W	PrimeSurface 384 well U White Plate	384	U bottom	0.1mL	1	20

• Radiation sterilized
• Preserved at room temperature, Product life: 2 years after production

PrimeSurface Cultureware White Plate FAQs

What coating is used on PrimeSurface® products (PrimeSurface® (Cultureware Dishes and Plates, White Plate, 96 Slit-well Plate)?
PrimeSurface® products are coated with a unique ultra-hydrophilic polymer that is covalently bound to the plastic surface and effectively inhibits cell attachment without cytotoxicity or material degradation. The superior coating technology and manufacturing process offer uniform spheroid/EB formation and a smooth surface for clear cell imaging.

What are the dimensions of PrimeSurface® products (PrimeSurface® (Cultureware Dishes and Plates, White Plate, 96 Slit-well Plate) to support instrument setup?
Please follow the link below to download the dimensions.
Link: S-BIO_PrimeSurface_ULA-Dimensions

What is the sterilization method used for PrimeSurface® products (PrimeSurface® (Cultureware Dishes and Plates, White Plate, 96 Slit-well Plate)? What is the expiration period?
PrimeSurface products are radiation sterilized after packaging. The expiration date is indicated on the product packaging. PrimeSurface products can be used for 2 to 3 years after production.
Sterilization is conducted according to the sterilization dose of SAL 10^-6 established by a method in accordance with ISO 11137-2/JIST0806-2.

What is the difference between PrimeSurface® flat dishes and PrimeSurface® culture plates?
PrimeSurface flat dishes (35, 60 and 90 mm) have flat bottoms and randomly generate multiple spheroids with different sizes, whereas PrimeSurface culture plates (96 and 384 wells) were designed to generate single and uniformly sized spheroids in every well.

How do I change the culture medium of cell spheroids when using PrimeSurface® products (PrimeSurface® (Cultureware Dishes and Plates, White Plate, 96 Slit-well Plate)?
We recommend changing half of the culture medium using a micropipette and handling the sample gently. Because the spheroids are non-adherent, they may be inadvertently removed during medium exchange. Avoid positioning the pipette tip close to the spheroid. Slow and careful aspiration is strongly recommended to minimize spheroid loss.

Are there any special instructions for using PrimeSurface® products (PrimeSurface® (Cultureware Dishes and Plates, White Plate, 96 Slit-well Plate)?
Yes. As the surface of each well is coated with a hydrophilic polymer, during seeding make sure pipette tips do not scratch the bottom or sides of the wells. Cells may attach to the well if there is a scratch.

Can I use a centrifuge to spin down cells when using PrimeSurface® products (PrimeSurface® (Cultureware Dishes and Plates, White Plate, 96 Slit-well Plate)?
Yes. A centrifugal force of 3000 × g was used in our internal studies; however, this is highly variable, depends on the cell type, and is not a guaranteed value.

How can I control the size of spheroids when using PrimeSurface® products (PrimeSurface® (Cultureware Dishes and Plates, White Plate, 96 Slit-well Plate)?
The diameter of the spheroids can be controlled by changing the initial seeding density.

What imaging systems have been used with PrimeSurface® products (PrimeSurface® (Cultureware Dishes and Plates, White Plate, 96 Slit-well Plate)?
The following imaging systems have been used with PrimeSurface® products:
・Olympus IX71
・Carl Zeiss LSM710
・Cell3iMager by SCREEN Holdings
・IncuCyte® by Sartorius
・Cytation by BioTek (now Agilent Technologies)
・Celligo by Nexcelom
・Opera Phenix by PerkinElmer