Product Description
PrimeSurface cultureware consists of ultra-low attachment (ULA) dishes and plates designed to promote scaffold-free, self-assembly spheroid formation with uniform size and shape.
These plates are pre-coated with a unique ultra-hydrophilic polymer that facilitates spontaneous spheroid formation. Their high optical clarity makes them highly suitable for bright-field imaging and confocal microscopy.
In addition to the commonly used 96-well U-bottom plates, spindle bottom and V-bottom 96-well plates are also available, allowing researchers to form aggregates required for specific cell types.
For high-throughput screening (HTS) applications, 384-well plates are also available.
Application
• Stem Cell Research: Induction of ES, iPS and mesenchymal stem cells differentiation by embryoid body formation.
• Drug Screening Research & Development: An ideal product for drug response studies. 3D spheroid models are more physiologically relevant than 2D monolayer model.
• Compatible with brightfield and fluorescence Imaging and Confocal microscopy:
• NoviSight 3D High Content Analysis by Olympus
• Celigo® by Nexcelom
• Spheroid applications on IncuCyte® by Sartorius
• Cytation 5 Biotek
• Tissue engineering and Regenerative medicine
Technical Data
• Generation of Human iPS Cell-Derived Kidney Organoids and Their Application in Drug Discovery Research
• Stimulus effects Evaluation
• Blood-brain barrier (BBB)
• Comparison data (3D spheroid culture using HepG2 cell line)
• For Tissue engineering and Regenerative medicine
• Application example of hES cells differentiation using PrimeSurface
The uniform spheroids form spontaneously within one day for effective aggregate formation of ES cells by using PrimeSurface
Please check the S-Bio’s website for more detail example of application.
Link: https://www.sumibe.co.jp/english/product/s-bio/cell-culture/primesurface-96u/index.html
Specifications
• Dish
| Cat. No | Product | Description | Culture Area | Qty/Pk | Qty/Cs |
| SBC-MS-90350 | PrimeSurface Dish 35 φ, clear | 35 Φ × 14(H)mm | 9 cm2 | 5 | 50 |
| SBC-MS-90600 | PrimeSurface Dish 60 φ, clear | 60 Φ × 15(H)mm | 21 cm2 | 10 | 120 |
| SBC-MS-90900 | PrimeSurface Dish 90 φ, clear | 90 Φ × 20(H)mm | 57 cm2 | 10 | 50 |
• Microplate
| Cat. No | Product | Wells | Bottom | Volume | Qty/Pk | Qty/Cs |
| SBC-MS-90240 | PrimeSurface Plate 24 well F plate, clear | 24 | Flat bottom | 3.4mL/well | 1 | 10 |
| SBC-MS-9096VZ | PrimeSurface 96 well V plate, clear | 96 | V bottom | 0.3mL | 1 | 20 |
| SBC-MS-9096MZ | PrimeSurface 96 well M plate, clear | 96 | Spindle bottom | 0.2mL | 1 | 20 |
| SBC-MS-9096UZ | PrimeSurface 96 well U plate, clear | 96 | U bottom | 0.3mL | 1 | 20 |
| SBC-MS-9384U | PrimeSurface 384well U plate, clear | 384 | U bottom | 0.1mL | 1 | 20 |
• Radiation sterilized
• Preserved at room temperature, Product life: 2 years after production
| Documents & Links for PrimeSurface Cultureware Dishes and Plates | |
| Manual | PrimeSurface Cultureware Dishes and Plates Manual |
| Documents & Links for PrimeSurface Cultureware Dishes and Plates | |
| Manual | PrimeSurface Cultureware Dishes and Plates Manual |
| Citations for PrimeSurface Cultureware Dishes and Plates – 1 Found |
| Ohnishi, Hiroe; Kawasaki, Takashi; Deguchi, Tomonori; Yuba, Shunsuke. Generation of Xeroderma Pigmentosum-A Patient-Derived Induced Pluripotent Stem Cell Line for Use As Future Disease Model. Cellular Reprogramming. 2015;17(4):268-74. PubMed |