PrimeSurface Cultureware Dishes and Plates

S-BIO

Catalog No.: SBC-MS-90350

Product Description

PrimeSurface cultureware consists of ultra-low attachment (ULA) dishes and plates designed to promote scaffold-free, self-assembly spheroid formation with uniform size and shape. These plates are pre-coated with a unique ultra-hydrophilic polymer that facilitates spontaneous spheroid formation. Their high optical clarity makes them highly suitable for bright-field imaging and confocal microscopy. In addition to the commonly used 96-well U-bottom plates, spindle bottom and V-bottom 96-well plates are also available, allowing researchers to form aggregates required for specific cell types. For high-throughput screening (HTS) applications, 384-well plates are also available.

Application

• Stem Cell Research: Induction of ES, iPS and mesenchymal stem cells differentiation by embryoid body formation.
• Drug Screening Research & Development: An ideal product for drug response studies. 3D spheroid models are more physiologically relevant than 2D monolayer model.
• Compatible with brightfield and fluorescence Imaging and Confocal microscopy:
    •    NoviSight 3D High Content Analysis by Olympus
    •    Celigo® by Nexcelom
    •    Spheroid applications on IncuCyte® by Sartorius
    •    Cytation 5 Biotek
• Tissue engineering and Regenerative medicine

Technical Data

•    Generation of Human iPS Cell-Derived Kidney Organoids and Their Application in Drug Discovery Research
•    Stimulus effects Evaluation
•    Blood-brain barrier (BBB)
•    Comparison data (3D spheroid culture using HepG2 cell line)
•    For Tissue engineering and Regenerative medicine
•    Application example of hES cells differentiation using PrimeSurface
     The uniform spheroids form spontaneously within one day for effective aggregate formation of ES cells by using PrimeSurface

Please check the S-Bio’s website for more detail example of application.
Link: https://www.sumibe.co.jp/english/product/s-bio/cell-culture/primesurface-96u/index.html

Specifications

• Dish

Cat. No	Product	Description	Culture Area	Qty/Pk	Qty/Cs
SBC-MS-90350	PrimeSurface Dish 35 φ, clear	35 Φ × 14(H)mm	9 cm2	5	50
SBC-MS-90600	PrimeSurface Dish 60 φ, clear	60 Φ × 15(H)mm	21 cm2	10	120
SBC-MS-90900	PrimeSurface Dish 90 φ, clear	90 Φ × 20(H)mm	57 cm2	10	50

• Microplate

Cat. No	Product	Wells	Bottom	Volume	Qty/Pk	Qty/Cs
SBC-MS-90240	PrimeSurface Plate 24 well F plate, clear	24	Flat bottom	3.4mL/well	1	10
SBC-MS-9096VZ	PrimeSurface 96 well V plate, clear	96	V bottom	0.3mL	1	20
SBC-MS-9096MZ	PrimeSurface 96 well M plate, clear	96	Spindle bottom	0.2mL	1	20
SBC-MS-9096UZ	PrimeSurface 96 well U plate, clear	96	U bottom	0.3mL	1	20
SBC-MS-9384U	PrimeSurface 384well U plate, clear	384	U bottom	0.1mL	1	20

• Radiation sterilized
• Preserved at room temperature, Product life: 2 years after production

Citations for PrimeSurface Cultureware Dishes and Plates – 17 Found

Ohnishi, Hiroe; Kawasaki, Takashi; Deguchi, Tomonori; Yuba, Shunsuke. Generation of Xeroderma Pigmentosum-A Patient-Derived Induced Pluripotent Stem Cell Line for Use As Future Disease Model. Cellular Reprogramming. 2015;17(4):268-74. 
- Comments: Generation of iPS Cell Research

Ohnishi, Hiroe; Oda, Yasuaki; Aoki, Tetsuhiro; Tadokoro, Mika; Katsube, Yoshihiro; Ohgushi, Hajime; Hattori, Koji; Yuba, Shunsuke. A comparative study of induced pluripotent stem cells generated from frozen, stocked bone marrow- and adipose tissue-derived mesenchymal stem cells. Journal Of Tissue Engineering And Regenerative Medicine. 2012;6(4):261-71. 
- Comments: Generation of iPS Cell Research

Aoki, Tetsuhiro; Ohnishi, Hiroe; Oda, Yasuaki; Tadokoro, Mika; Sasao, Mari; Kato, Hiroyuki; Hattori, Koji; Ohgushi, Hajime. Generation of induced pluripotent stem cells from human adipose-derived stem cells without c-MYC. Tissue Engineering. Part A. 2010;16(7):2197-206. 
- Comments: Generation of iPS Cell Research

Oda, Yasuaki; Yoshimura, Yasuhide; Ohnishi, Hiroe; Tadokoro, Mika; Katsube, Yoshihiro; Sasao, Mari; Kubo, Yoko; Hattori, Koji; Saito, Shigeru; Horimoto, Katsuhisa; Yuba, Shunsuke; Ohgushi, Hajime. Induction of pluripotent stem cells from human third molar mesenchymal stromal cells. The Journal Of Biological Chemistry. 2010;285(38):29270-8. 
- Comments: Generation of iPS Cell Research

Murata, Daiki; Tokunaga, Satoshi; Tamura, Tadashi; Kawaguchi, Hiroaki; Miyoshi, Noriaki; Fujiki, Makoto; Nakayama, Koichi; Misumi, Kazuhiro. A preliminary study of osteochondral regeneration using a scaffold-free three-dimensional construct of porcine adipose tissue-derived mesenchymal stem cells. Journal Of Orthopaedic Surgery And Research. 2015;10:35. 
- Comments: Bone and Cartilage Research

Sato, Kazutoshi; Itoh, Takehiro; Kato, Toshiki; Kitamura, Yukiko; Kaul, Sunil C; Wadhwa, Renu; Sato, Fujio; Ohneda, Osamu. Serum-free isolation and culture system to enhance the proliferation and bone regeneration of adipose tissue-derived mesenchymal stem cells. In Vitro Cellular & Developmental Biology. Animal. 2015;51(5):515-29. 
- Comments: Bone and Cartilage Research

Fujimoto, Mai; Ohte, Satoshi; Shin, Masashi; Yoneyama, Katsumi; Osawa, Kenji; Miyamoto, Arei; Tsukamoto, Sho; Mizuta, Takato; Kokabu, Shoichiro; Machiya, Aiko; Okuda, Akihiko; Suda, Naoto; Katagiri, Takenobu. Establishment of a novel model of chondrogenesis using murine embryonic stem cells carrying fibrodysplasia ossificans progressiva-associated mutant ALK2. Biochemical And Biophysical Research Communications. 2014;455(3-4):347-52. 
- Comments: Bone and Cartilage Research

Ishihara, Kohei; Nakayama, Koichi; Akieda, Shizuka; Matsuda, Shuichi; Iwamoto, Yukihide. Simultaneous regeneration of full-thickness cartilage and subchondral bone defects in vivo using a three-dimensional scaffold-free autologous construct derived from high-density bone marrow-derived mesenchymal stem cells. Journal Of Orthopaedic Surgery And Research. 2014;9:98. 
- Comments: Bone and Cartilage Research

Muguruma, Keiko; Nishiyama, Ayaka; Kawakami, Hideshi; Hashimoto, Kouichi; Sasai, Yoshiki. Self-organization of polarized cerebellar tissue in 3D culture of human pluripotent stem cells. Cell Reports. 2015;10(4):537-50. 
- Comments: Neuroscience Research

Bamba, Yohei; Shofuda, Tomoko; Kanematsu, Daisuke; Nonaka, Masahiro; Yamasaki, Mami; Okano, Hideyuki; Kanemura, Yonehiro. Differentiation, polarization, and migration of human induced pluripotent stem cell-derived neural progenitor cells co-cultured with a human glial cell line with radial glial-like characteristics. Biochemical And Biophysical Research Communications. 2014;447(4):683-8. 
- Comments: Neuroscience Research

Minamino, Yuki; Ohnishi, Yuichi; Kakudo, Kenji; Nozaki, Masami. Isolation and propagation of neural crest stem cells from mouse embryonic stem cells via cranial neurospheres. Stem Cells And Development. 2015;24(2):172-81. 
- Comments: Neuroscience Research

Kadoshima, Taisuke; Sakaguchi, Hideya; Nakano, Tokushige; Soen, Mika; Ando, Satoshi; Eiraku, Mototsugu; Sasai, Yoshiki. Self-organization of axial polarity, inside-out layer pattern, and species-specific progenitor dynamics in human ES cell-derived neocortex. Proceedings Of The National Academy Of Sciences Of The United States Of America. 2013;110(50):20284-9. 
- Comments: Neuroscience Research

Ogawa, Yasuhiro; Tanaka, Makoto; Tanabe, Miho; Suzuki, Toshihiro; Togawa, Tadayasu; Fukushige, Tomoko; Kanekura, Takuro; Sakuraba, Hitoshi; Oishi, Kazuhiko. Impaired neural differentiation of induced pluripotent stem cells generated from a mouse model of Sandhoff disease. Plos One. 8(1):e55856. 
- Comments: Neuroscience Research

Gomi, Masanori; Takagi, Yasushi; Morizane, Asuka; Doi, Daisuke; Nishimura, Masaki; Miyamoto, Susumu; Takahashi, Jun. Functional recovery of the murine brain ischemia model using human induced pluripotent stem cell-derived telencephalic progenitors. Brain Research. 2012;1459:52-60. 
- Comments: Neuroscience Research

Nasu, Makoto; Takata, Nozomu; Danjo, Teruko; Sakaguchi, Hideya; Kadoshima, Taisuke; Futaki, Sugiko; Sekiguchi, Kiyotoshi; Eiraku, Mototsugu; Sasai, Yoshiki. Robust formation and maintenance of continuous stratified cortical neuroepithelium by laminin-containing matrix in mouse ES cell culture. Plos One. 7(12):e53024. 
- Comments: Neuroscience Research

Danjo, Teruko; Eiraku, Mototsugu; Muguruma, Keiko; Watanabe, Kiichi; Kawada, Masako; Yanagawa, Yuchio; Rubenstein, John L R; Sasai, Yoshiki. Subregional specification of embryonic stem cell-derived ventral telencephalic tissues by timed and combinatory treatment with extrinsic signals. The Journal Of Neuroscience : The Official Journal Of The Society For Neuroscience. 2011;31(5):1919-33. 
- Comments: Neuroscience Research

Kanemura, Yonehiro. Development of cell-processing systems for human stem cells (neural stem cells, mesenchymal stem cells, and iPS cells) for regenerative medicine. The Keio Journal Of Medicine. 59(2):35-45. 
- Comments: Neuroscience Research

PrimeSurface Cultureware Dishes and Plates FAQs

What coating is used on PrimeSurface® products (PrimeSurface® (Cultureware Dishes and Plates, White Plate, 96 Slit-well Plate)?
PrimeSurface® products are coated with a unique ultra-hydrophilic polymer that is covalently bound to the plastic surface and effectively inhibits cell attachment without cytotoxicity or material degradation. The superior coating technology and manufacturing process offer uniform spheroid/EB formation and a smooth surface for clear cell imaging.

What are the dimensions of PrimeSurface® products (PrimeSurface® (Cultureware Dishes and Plates, White Plate, 96 Slit-well Plate) to support instrument setup?
Please follow the link below to download the dimensions.
S-BIO_PrimeSurface_ULA-Dimensions

What is the sterilization method used for PrimeSurface® products (PrimeSurface® (Cultureware Dishes and Plates, White Plate, 96 Slit-well Plate)? What is the expiration period?
PrimeSurface products are radiation sterilized after packaging. The expiration date is indicated on the product packaging. PrimeSurface products can be used for 2 to 3 years after production.
Sterilization is conducted according to the sterilization dose of SAL 10^-6 established by a method in accordance with ISO 11137-2/JIST0806-2.

What is the difference between PrimeSurface® flat dishes and PrimeSurface® culture plates?
PrimeSurface flat dishes (35, 60 and 90 mm) have flat bottoms and randomly generate multiple spheroids with different sizes, whereas PrimeSurface culture plates (96 and 384 wells) were designed to generate single and uniformly sized spheroids in every well.

How do I change the culture medium of cell spheroids when using PrimeSurface® products (PrimeSurface® (Cultureware Dishes and Plates, White Plate, 96 Slit-well Plate)?
We recommend changing half of the culture medium using a micropipette and handling the sample gently. Because the spheroids are non-adherent, they may be inadvertently removed during medium exchange. Avoid positioning the pipette tip close to the spheroid. Slow and careful aspiration is strongly recommended to minimize spheroid loss.

Are there any special instructions for using PrimeSurface® products (PrimeSurface® (Cultureware Dishes and Plates, White Plate, 96 Slit-well Plate)?
Yes. As the surface of each well is coated with a hydrophilic polymer, during seeding make sure pipette tips do not scratch the bottom or sides of the wells. Cells may attach to the well if there is a scratch.

Can I use a centrifuge to spin down cells when using PrimeSurface® products (PrimeSurface® (Cultureware Dishes and Plates, White Plate, 96 Slit-well Plate)?
Yes. A centrifugal force of 3000 × g was used in our internal studies; however, this is highly variable, depends on the cell type, and is not a guaranteed value.

How can I control the size of spheroids when using PrimeSurface® products (PrimeSurface® (Cultureware Dishes and Plates, White Plate, 96 Slit-well Plate)?
The diameter of the spheroids can be controlled by changing the initial seeding density.

What imaging systems have been used with PrimeSurface® products (PrimeSurface® (Cultureware Dishes and Plates, White Plate, 96 Slit-well Plate)?
The following imaging systems have been used with PrimeSurface® products:
・Olympus IX71
・Carl Zeiss LSM710
・Cell3iMager by SCREEN Holdings
・IncuCyte® by Sartorius
・Cytation by BioTek (now Agilent Technologies)
・Celligo by Nexcelom
・Opera Phenix by PerkinElmer