Product Description

PrimeSurface cultureware consists of ultra-low attachment (ULA) dishes and plates designed to promote scaffold-free, self-assembly spheroid formation with uniform size and shape. These plates are pre-coated with a unique ultra-hydrophilic polymer that facilitates spontaneous spheroid formation. Their high optical clarity makes them highly suitable for bright-field imaging and confocal microscopy. In addition to the commonly used 96-well U-bottom plates, spindle bottom and V-bottom 96-well plates are also available, allowing researchers to form aggregates required for specific cell types. For high-throughput screening (HTS) applications, 384-well plates are also available.

Application

• Stem Cell Research: Induction of ES, iPS and mesenchymal stem cells differentiation by embryoid body formation.
• Drug Screening Research & Development: An ideal product for drug response studies. 3D spheroid models are more physiologically relevant than 2D monolayer model.
• Compatible with brightfield and fluorescence Imaging and Confocal microscopy:
    •    NoviSight 3D High Content Analysis by Olympus
    •    Celigo® by Nexcelom
    •    Spheroid applications on IncuCyte® by Sartorius
    •    Cytation 5 Biotek
• Tissue engineering and Regenerative medicine

Technical Data

•    Generation of Human iPS Cell-Derived Kidney Organoids and Their Application in Drug Discovery Research
•    Stimulus effects Evaluation
•    Blood-brain barrier (BBB)
•    Comparison data (3D spheroid culture using HepG2 cell line)
•    For Tissue engineering and Regenerative medicine
•    Application example of hES cells differentiation using PrimeSurface
     The uniform spheroids form spontaneously within one day for effective aggregate formation of ES cells by using PrimeSurface

Please check the S-Bio’s website for more detail example of application.
Link: https://www.sumibe.co.jp/english/product/s-bio/cell-culture/primesurface-96u/index.html

Specifications

• Dish

Cat. No Product Description Culture Area Qty/Pk Qty/Cs
SBC-MS-90350 PrimeSurface Dish 35 φ, clear 35 Φ × 14(H)mm 9 cm2 5 50
SBC-MS-90600 PrimeSurface Dish 60 φ, clear 60 Φ × 15(H)mm 21 cm2 10 120
SBC-MS-90900 PrimeSurface Dish 90 φ, clear 90 Φ × 20(H)mm 57 cm2 10 50

• Microplate

Cat. No Product Wells Bottom Volume Qty/Pk Qty/Cs
SBC-MS-90240 PrimeSurface Plate 24 well F plate, clear 24 Flat bottom 3.4mL/well 1 10
SBC-MS-9096VZ PrimeSurface 96 well V plate, clear 96 V bottom 0.3mL 1 20
SBC-MS-9096MZ PrimeSurface 96 well M plate, clear 96 Spindle bottom 0.2mL 1 20
SBC-MS-9096UZ PrimeSurface 96 well U plate, clear 96 U bottom 0.3mL 1 20
SBC-MS-9384U PrimeSurface 384well U plate, clear 384 U bottom 0.1mL 1 20

• Radiation sterilized
• Preserved at room temperature, Product life: 2 years after production




Documents & Links for PrimeSurface Cultureware Dishes and Plates
Manual PrimeSurface Cultureware Dishes and Plates Manual

Documents & Links for PrimeSurface Cultureware Dishes and Plates
Manual PrimeSurface Cultureware Dishes and Plates Manual
Citations for PrimeSurface Cultureware Dishes and Plates – 1 Found
Ohnishi, Hiroe; Kawasaki, Takashi; Deguchi, Tomonori; Yuba, Shunsuke. Generation of Xeroderma Pigmentosum-A Patient-Derived Induced Pluripotent Stem Cell Line for Use As Future Disease Model. Cellular Reprogramming. 2015;17(4):268-74.  PubMed