PrimeSurface Cultureware 96 Slit-well Plate

S-BIO

Catalog No.: SBC-MS-9096S

Product Description

PrimeSurface 96 Slit-well plate is a newly designed ultra-low attachment (ULA) 3D plate for spheroid cell culture that improves media exchange handling without disrupting spheroid formation.

Spheroid cell culture requires frequent media replacement to supply nutrients to growing cells. In conventional 96-well ULA plates, careful media aspiration or dispensing is necessary to avoid disturbing unattached spheroids, making the process time-consuming. The PrimeSurface 96 Slit-well Plate allows one-step media dispensing or aspiration for all 96 wells simultaneously, reducing pipetting time by over 80% while minimizing spheroid damage risk. This enhancement significantly improves stem cell culture efficiency in regenerative medicine applications.

Key Benefits

• One‑step, uniform media exchange across 96 wells
• Ultra‑low attachment surface supports stable, reproducible spheroid formation
• Minimizes spheroid disruption during frequent media changes
• Reduces hands‑on time and manual pipetting by 80%+
• Improves consistency in stem cell and 3D culture workflows

Application

• Stem cell research
• Regenerative medicine
• Drug discovery and screening
• Long‑term spheroid maintenance

Specifications

Cat. No.	Product	Wells	Volume	Qty/Pk	Qty/Cs
SBC-MS-9096SZ	PrimeSurface 96 well U White Plate	96	0.3mL	1	20

• Radiation sterilized
• Preserved at room temperature, Product life: 2 years after production

 

PrimeSurface Cultureware 96 Slit-well Plate FAQs

What coating is used on PrimeSurface® products (PrimeSurface® (Cultureware Dishes and Plates, White Plate, 96 Slit-well Plate?
PrimeSurface® products are coated with a unique ultra-hydrophilic polymer that is covalently bound to the plastic surface and effectively inhibits cell attachment without cytotoxicity or material degradation. The superior coating technology and manufacturing process offer uniform spheroid/EB formation and a smooth surface for clear cell imaging.

What are the dimensions of PrimeSurface® products (PrimeSurface® (Cultureware Dishes and Plates, White Plate, 96 Slit-well Plate) to support instrument setup?
Please follow the link below to download the dimensions.
Link: S-BIO_PrimeSurface_ULA-Dimensions

What is the sterilization method used for PrimeSurface® products (PrimeSurface® (Cultureware Dishes and Plates, White Plate, , 96 Slit-well Plate)? What is the expiration period?
PrimeSurface products are radiation sterilized after packaging. The expiration date is indicated on the product packaging. PrimeSurface products can be used for 2 to 3 years after production.
Sterilization is conducted according to the sterilization dose of SAL 10^-6 established by a method in accordance with ISO 11137-2/JIST0806-2.

What is the difference between PrimeSurface® flat dishes and PrimeSurface® culture plates?
PrimeSurface flat dishes (35, 60 and 90 mm) have flat bottoms and randomly generate multiple spheroids with different sizes, whereas PrimeSurface culture plates (96 and 384 wells) were designed to generate single and uniformly sized spheroids in every well.

How do I change the culture medium of cell spheroids when using PrimeSurface® products (PrimeSurface® (Cultureware Dishes and Plates, White Plate, 96 Slit-well Plate)?
We recommend changing half of the culture medium using a micropipette and handling the sample gently. Because the spheroids are non-adherent, they may be inadvertently removed during medium exchange. Avoid positioning the pipette tip close to the spheroid. Slow and careful aspiration is strongly recommended to minimize spheroid loss.

Are there any special instructions for using PrimeSurface® products (PrimeSurface® (Cultureware Dishes and Plates, White Plate, 96 Slit-well Plate)?
Yes. As the surface of each well is coated with a hydrophilic polymer, during seeding make sure pipette tips do not scratch the bottom or sides of the wells. Cells may attach to the well if there is a scratch.

Can I use a centrifuge to spin down cells when using PrimeSurface® products (PrimeSurface® (Cultureware Dishes and Plates, White Plate, 96 Slit-well Plate)?
Yes. A centrifugal force of 3000 × g was used in our internal studies; however, this is highly variable, depends on the cell type, and is not a guaranteed value.

How can I control the size of spheroids when using PrimeSurface® products (PrimeSurface® (Cultureware Dishes and Plates, White Plate, 96 Slit-well Plate>)?
The diameter of the spheroids can be controlled by changing the initial seeding density.

What imaging systems have been used with PrimeSurface® products (PrimeSurface® (Cultureware Dishes and Plates, White Plate, 96 Slit-well Plate)?
The following imaging systems have been used with PrimeSurface® products:
・Olympus IX71
・Carl Zeiss LSM710
・Cell3iMager by SCREEN Holdings
・IncuCyte® by Sartorius
・Cytation by BioTek (now Agilent Technologies)
・Celligo by Nexcelom
・Opera Phenix by PerkinElmer