Maintaining and propagating valuable mouse lines through in vitro fertilization (IVF) is essential for advancing biomedical research. However, certain strains — especially C57BL/6 — are notoriously inefficient in standard IVF protocols.
Cosmo Bio USA offers a complete solution with Kyudo/Card reagents, designed to improve mouse sperm cryopreservation, thawing, and IVF outcomes. These reagents significantly increase fertilization rates using both frozen-thawed and fresh sperm, even in challenging strains.
Our Reagent System includes:
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CARD FERTIUP® CPA: A cryoprotectant that protects mouse spermatozoa during freezing and thawing, reducing cellular damage and preserving fertility potential.
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CARD FERTIUP® PM: A pre-incubation medium that enhances the fertilizing ability of mouse sperm before IVF.
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CARD MEDIUM®: A high-performance fertilization medium that acts on the oocyte pellucida to improve oocyte receptivity and fertilization success.
Used together, these reagents enable efficient, low-cost cryopreservation and IVF for transgenic (Tg), knockout (KO), and IVF-resistant mouse lines — helping researchers preserve genetic integrity and accelerate their studies.