Yatalase, Fungal Cell Lytic Enzymes

Ozeki

Catalog No.: OZK-OZ-10EX

Background

Yatalase is used to lyse cell walls of filamentous fungi. The product is prepared from culture supernatants of Corynebacterium sp. OZ-21, and consists mainly of chitinase, chitobiase and β-1,3-glucanase.

 

 

Features

Has excellent thermostability and can be stored at room temperature. Efficiently digests native chitin. Has the revitalization of Chitinase, Chitobiase, Chitosanase, and β-1,3-Glucanarse Can be used alone to prepare protoplasts from filamentous fungi.

 

Source

Corynebacterium sp. OZ-21 Form : Lyophilized powder(containing lactose)

 

Storage

Lyophilized preparation is stable for at least 1 year at 4°C.
Maintain at -20°C for long term strage. Avoid freeze/thaw cycles.

 

Specific activities

Chitinase activity : Approximately 50 units/g powder
Chitobiase activity : Approximately 500 units/g powder Lytic activity against cell walls : Approximately 10,000 units/g powder

 

Properties

Optimum pH : pH 5〜8 Optimum temperature for enzyme activity : 30〜50℃

Unit definition

Chitinase activity: One unit of chitinase activity is determined as the amount required to release 1µmol of N-acetylglucosamine from chitin in 1 minute.
Chitobiase activity: One unit of chitobiase activity is defined as the amount of enzyme required to release 1µmol of p-nitrophenol from p-nitrophenyl-N-acetyl-β-D-glucosaminide in 1 minute. Lytic activity: One unit of enzyme activity is defined as the amount required to cause a 1% decrease in absorbance in 1 hour.

A Frequently Asked Question

Is it effective against any type of filamentous fungus?

Aspergillus oryzaewithTrichoderma reesei,T.viride,T. koningiican be used to prepare protoplasts very efficiently and in reduced amounts.
OtherAspergillusIf protoplasts are difficult to obtain from Ascomycetes, Zygomycetes, and Basidiomycetes, including those of the genus Pseudomonas, we recommend using it in combination with 0.5% cellulase "Onozuka" R-10.

Citations for Yatalase, Fungal Cell Lytic Enzymes – 0 Found

Reissig, J. L., Strominger, J. L. and Leloir, L. F.. J. Biol. Chem.. 1955;217:959. 

Fujii, F., Ozeki, K., Kanda, A., Hamaji, M., and Nunokawa, Y.. 1998;87:757. 

Ozeki, K., Ishimaru (Nagamasu), Y., Koda, A., Minetoki, T., Obuchi, K., Hamaji, M., and Kumagai, C. . 1998;76:187.