SAINOME 384-Well Format Gel Excision Tool

sainome

Catalog No.: SAI-SAI-386-B

Product Description

SAINOME® is a useful analysis tool for proteome analysis and DEG assay (Diced Electrophoresis Gel assay). The gel after electrophoresis can be dispensed into a 384-well microplate and used for reaction or measurement using a microplate reader. DEG Assay is a new analytical method that can detect with high sensitivity a trace amount of enzyme activity that could not be detected by conventional methods. With SAINOME®, DEG Assays can be performed efficiently and reproducibly. 

SAINOME® was developed under the guidance of Dr. Toru Komatsu of the University of Tokyo Graduate School of Pharmaceutical Sciences. Domestic patent registered (Patent No. 5594501), PCT international application completed, domestic trademark registered.

How to use SAINOME® : DEG Assay

DEG Assay

DEG Assay is a novel analytical method that can detect trace amounts of enzyme activity that could not be detected by conventional methods with high sensitivity. By using SAINOME®, DEG Assay can be performed efficiently with high reproducibility.

A novel research method of omics analysis (Enzymomics) that focuses on enzymes.

This method comprehensively detects only the proteins with the desired enzyme activity within the proteome.
It can even detect minute amounts of protein, less than 0.1 ng.

 

Examples of enzyme probes and their sensitivity

	Enzyme	Probe	Detection Limit
Glycosidases	β-Galactosidase	TGβ-Gal	0.03ng
	β-Glucosidase	TGβ-Glc	1ng
Esterases	Alkaline Phosphatase	Umb Phos	0.3ng
	Porcine Liver esterase (PLE)	Fluorescin Dibutyrase (FDB)	10ng
Peptidases	Elastase	Meo-Suc-Ala-Ala-Pro-lle-AMC	3ng
	Leucine Aminopeptidase (LAP)	Leu-AMC	0.3ng

Detection of enzyme activity by isozyme

We introduce over 129 types of enzyme probes, tailored to specific enzymes.

We can also meet your needs for detecting various enzyme activities.

We also support the search for enzymes that control phenotypes at the cellular level
(e.g., activity evaluation of APEH, an enzyme involved in the metabolism of N-formylmethionine-containing peptides).

Application examples: It can be used in various basic and clinical research.

• Discovery of a 2-formylated peptide metabolizing enzyme
• Comprehensive analysis of enzyme activity involved in the metabolism of oxidatively modified proteins (see figure below)
• Detection of alkaline phosphatase activity in serum

References

1. Kentaro Yoshioka, Toru Komatsu, Kenjiro Hanaoka, Tasuku Ueno, Takuya Terai, Tetsuo Nagano, and Yasuteru Urano, Chem. Commun., 52, 4377-4380 (2016)
2. Kentaro Yoshioka, Toru Komatsu, Akihiro Nakada, Jun Onagi, Yugo Kuriki, Mitsuyasu Kawaguchi, Takuya Terai, Tasuku Ueno, Kenjiro Hanaoka, Tetsuo Nagano, and Yasuteru Urano, J. Am. Chem. Soc., 2015, 137, 12187–12190
3. Toru Komatsu and Tetsuo Nagano, MEDCHEM NEWS No. 3 AUGUST 2013. 33
4. Toru Komatsu, Kenjiro Hanaoka, Alexander Adibekian, Kentaro Yoshioka, Takuya Terai, Tasuku Ueno, Mitsuyasu Kawaguchi, Benjamin F. Cravatt, and Tetsuo Nagano, J. Am. Chem. Soc. 2013, 135, 6002-6005