KP293 type 2 Expression medium

Kyokuto Pharmaceutical Indust

Catalog No.: KYO-29335

Product Description

This product is a culture medium for suspension culture of human embryonic kidney cell-derived 293 cells (e.g., Expi293F cells, 293T cells, etc.). It can be used for the expression and production of recombinant proteins using 293 cells. It enables stable subculturing and achieves high transfection efficiency.

 

Features

• Manufactured in our own factory in Japan.
• Transfection is possible using lipofection or polyethyleneimine methods.
• serum-free culture medium with known chemical composition
• Free of animal-derived ingredients and proteins.

Examples of proteins expressed using this product

• GFP ( Green Fluorescent Protein )*
• Secretory alkaline phosphatase
• PLXDC2 extracellular protein (secreted, human, mouse type) *
• Three types of SARS-CoV-2 proteins (NP, ORF 3b, ORF8)
• Rabbit monoclonal antibody (secretion) *

References

• Nakagawa M. et al. (2023) Electrophoresis 44:1446-1460.
• Sakuma C. et al. (2022) Biotechniques 72:207-218.
• Arakawa T. et al. (2022) Methods in Cell Biology 169:67-95.
• Nakagawa M. et al. (2021) Int J Biol Macromol 189:869-878.
• Sakuma C. et al. (2021) Int J Biol Macromol 172:589-596.
• Sakuma C. et al. (2021) Int J Biol Macromol 166:1106-1110.
• Li C. et al. (2020) 151:885-890.
• Riki Arakawa, Teruo Akuta (2021) Biochemistry 9:566-572.

Frequently Asked Questions

Q1. Does this product contain glutamine?

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This product contains L-Alanyl-L-Glutamine, so there is no need to add L-Glutamine separately.

Q2. Is adaptation from other culture media possible?

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The protocol includes instructions on how to acclimate the system, so please refer to that.

Q3. Which cell lines have proven successful with this product?

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These are Expi293F and 293T cells. We plan to increase the number of cases in the future.

Q4. Are there any precautions regarding storage?

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Please store this product in a refrigerator and in a dark place.