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Hyaluronan (HA) is an unbranched glycosaminoglycan composed of repeating D-glucuronic acid—N-acetyl-D-glucosamine disaccharide subunits. Hyaluronan Quantification Kit is a highly specific, competitive ELISA kit featuring an HA binding protein optimized to quantify HA polymers larger than 7.4 kDa in samples such as serum, plasma and culture supernatants.
Hyaluronan (HA) is an unbranched glycosaminoglycan composed of repeating D-glucuronic acid—N-acetyl-D-glucosamine disaccharide subunits. HA is abundant in synovial fluid, skin, umbilical cord, and vitreous bodies. HA is a prominent component of the extracellular matrix, contributing to tissue water retention, cellular growth, differentiation, and migration. Derived from the activity of several distinct HA synthases and degradation enzymes, HA exists in the body in a strikingly wide range of molecular weights. For example, the average molecular weights of HA in synovial fluid, blood and urine are 4-6 MDa, 100-300 kDa and less 10 kDa, respectively.
Features
- Minimally affected by the molecular size of HA
- Accurately quantitate HA in blood serum, plasma and supernatants where the molecular weight is above 7.4 kDa
- Quantitate HA concentrations between 3.13~200 ng/ml
Specificity
Hyaluronan Quantification Kit does not exhibit cross-reactivity with chondroitin sulfate-A (CS-A), CS- C, CS-D, CS-E, heparan sulfate (HS), heparin (Hep), keratan sulfate (KS) and keratan polysulfate (KPS) at concentrations up to 1000 ng/mL. Weak reactivity is detected for high concentrations of CS-B (dermatan sulfate).
Kit Contents
| Description | Volume | Quantity |
|---|---|---|
| 96 well plate | 8 x 12 wells | 1 |
| HA Coating Solution | 11 mL | 1 |
| Wash Buffer (20X) | 50 mL | 1 |
| Sample Buffer (2X) * | 50 mL | 1 |
| Blocking Buffer (2X) * | 11 mL | 1 |
| HA Standard (10 µg/mL) * | 0.1 mL | 1 |
| 96 well plate | 8 x 12 wells | 1 |
| Biotin-HABP * | 10 - 40 µL | 1 |
| HRP-Avidin (100X) * | 0.2 mL | 1 |
| Substrate Solution A | 1 mL | 1 |
| Substrate Solution B | 10 mL | 1 |
| Stop Solution | 11 mL | 1 |
| Plate Seals | 5 mL | 2 |
| 96 well plate | 8 x 12 wells | 1 |
* : containing 0.025 - 0.05% Proclin 300
| Documents & Links for Hyaluronan Quantification Kit | |
| Datasheet | csr-ha-96kit_hyaluronan-quantification-kit_datasheet.pdf |
| Vendor Page | Hyaluronan Quantification Kit at Cosmo Bio LTD |
| Documents & Links for Hyaluronan Quantification Kit | |
| Datasheet | csr-ha-96kit_hyaluronan-quantification-kit_datasheet.pdf |
| Vendor Page | Hyaluronan Quantification Kit at Cosmo Bio LTD |