Hyaluronan Quantification Kit

Cosmo Bio

Catalog No.: CSR-HA-96KIT

Background

Hyaluronan (HA) is an unbranched glycosaminoglycan composed of repeating D-glucuronic acid—N-acetyl-D-glucosamine disaccharide subunits. Hyaluronan Quantification Kit is a highly specific, competitive ELISA kit featuring an HA binding protein optimized to quantify HA polymers larger than 7.4 kDa in samples such as serum, plasma and culture supernatants.

Hyaluronan (HA) is an unbranched glycosaminoglycan composed of repeating D-glucuronic acid—N-acetyl-D-glucosamine disaccharide subunits. HA is abundant in synovial fluid, skin, umbilical cord, and vitreous bodies. HA is a prominent component of the extracellular matrix, contributing to tissue water retention, cellular growth, differentiation, and migration. Derived from the activity of several distinct HA synthases and degradation enzymes, HA exists in the body in a strikingly wide range of molecular weights. For example, the average molecular weights of HA in synovial fluid, blood and urine are 4-6 MDa, 100-300 kDa and less 10 kDa, respectively.

Features

• Minimally affected by the molecular size of HA
• Accurately quantitate HA in blood serum, plasma and supernatants where the molecular weight is above 7.4 kDa
• Quantitate HA concentrations between 3.13~200 ng/ml

Specificity

Hyaluronan Quantification Kit does not exhibit cross-reactivity with chondroitin sulfate-A (CS-A), CS- C, CS-D, CS-E, heparan sulfate (HS), heparin (Hep), keratan sulfate (KS) and keratan polysulfate (KPS) at concentrations up to 1000 ng/mL. Weak reactivity is detected for high concentrations of CS-B (dermatan sulfate).

Kit Contents

Component	Volume	Quantity
96 well plate	8 x 12 wells	1
HA Coating Solution	11 mL	1
Wash Buffer (20X)	50 mL	1
Sample Buffer (2X) *	50 mL	1
Blocking Buffer (2X) *	11 mL	1
HA Standard (10 µg/mL) *	0.1 mL	1
96 well plate	8 x 12 wells	1
Biotin-HABP *	10 - 40 µL	1
HRP-Avidin (100X) *	0.2 mL	1
Substrate Solution A	1 mL	1
Substrate Solution B	10 mL	1
Stop Solution	11 mL	1
Plate Seals	5 mL	2
96 well plate	8 x 12 wells	1

* : containing 0.025 - 0.05% Proclin 300

Citations for Hyaluronan Quantification Kit – 17 Found

Tsuboi, Eri; Asakawa, Yuki; Hirose, Naoto; Yanoshita, Makoto; Sumi, Chikako; Takano, Mami; Onishi, Azusa; Nishiyama, Sayuri; Kubo, Naoki; Kita, Daiki; Tanimoto, Kotaro. The role of semaphorin 3A on chondrogenic differentiation. In Vitro Cellular & Developmental Biology. Animal. 2024; 38727898( 38727898) 

Saga, Ryo; Iwamori, Kenta; Hosokawa, Yoichiro. Stromal-derived high-molecular-weight hyaluronan mediates radioresistance in the prostate cancer microenvironment. International Journal Of Radiation Biology. 102(2):138-144. 

Hsu, Peihan; Kamijyo, Yuriko; Koike, Emiri; Ichikawa, Saki; Zheng, Yifeng; Ohno, Tomohiro; Katayama, Shigeru. Exosome-like nanovesicles derived from kale juice enhance collagen production by downregulating Smad7 in human skin fibroblasts. Frontiers In Nutrition. 12:1486572. 

Bernardy, Jan; Fritz, Klaus; Salavastru, Carmen; Jarosova, Rea; Kralova, Natalie. Radiofrequency and targeted ultrasound enhance natural hyaluronic production: a pilot porcine study. Journal Of Ultrasound. 2025;28(4):1001-1006. 

Tsuboi, Eri; Asakawa, Yuki; Hirose, Naoto; Yanoshita, Makoto; Sumi, Chikako; Takano, Mami; Onishi, Azusa; Nishiyama, Sayuri; Kubo, Naoki; Kita, Daiki; Tanimoto, Kotaro. The role of semaphorin 3A on chondrogenic differentiation. In Vitro Cellular & Developmental Biology. Animal. 2024;60(6):609-615. 

Yamaguchi, Makoto; Mizuno, Masashi; Kitamura, Fumiya; Iwagaitsu, Shiho; Nobata, Hironobu; Kinashi, Hiroshi; Banno, Shogo; Asai, Akimasa; Ishimoto, Takuji; Katsuno, Takayuki; Ito, Yasuhiko. Case report: Thrombotic microangiopathy concomitant with macrophage activation syndrome in systemic lupus erythematosus refractory to conventional treatment successfully treated with eculizumab. Frontiers In Medicine. 9:1097528. 

Alhasan, M Anas; Tomokiyo, Atsushi; Hamano, Sayuri; Sugii, Hideki; Ono, Taiga; Ipposhi, Keita; Yamashita, Kozue; Mardini, Bara; Minowa, Fumiko; Maeda, Hidefumi. Hyaluronic Acid Induction Promotes the Differentiation of Human Neural Crest-like Cells into Periodontal Ligament Stem-like Cells. Cells. 2023;12(23) 

Hamester, Fabienne; Stürken, Christine; Legler, Karen; Eylmann, Kathrin; Möller, Katrin; Roßberg, Maila; Gorzelanny, Christian; Bauer, Alexander T; Windhorst, Sabine; Schmalfeldt, Barbara; Laakmann, Elena; Müller, Volkmar; Witzel, Isabell; Oliveira-Ferrer, Leticia. Key Role of Hyaluronan Metabolism for the Development of Brain Metastases in Triple-Negative Breast Cancer. Cells. 2022;11(20) 

Burukçu, Derya; Yilmaz, Sema; Alagöz, Oya; Yaltirik, Cumhur Kaan; Attar, Erkut; Şahin, Fikrettin; Aydemir, Esra. The investigation of diverse physiological and therapeutic impact of cellular-based products derived from human cumulus cells. Turkish Journal Of Biology = Turk Biyoloji Dergisi. 46(5):400-413. 

Marunaka, Kana; Shu, Shokoku; Kobayashi, Mao; Goto, Makiko; Katsuta, Yuji; Yoshino, Yuta; Ikari, Akira. Elevation of Hyaluronan Synthase by Magnesium Supplementation Mediated through the Activation of GSK3 and CREB in Human Keratinocyte-Derived HaCaT Cells. International Journal Of Molecular Sciences. 2021;23(1) 

Asai, Akimasa; Hatayama, Naoyuki; Kamiya, Keisuke; Yamauchi, Mai; Kinashi, Hiroshi; Yamaguchi, Makoto; Katsuno, Takayuki; Nobata, Hironobu; Watanabe, Kazushi; Wakatsuki, Akihiko; Aten, Jan; Maruyama, Shoichi; Ishimoto, Takuji; Hirai, Shuichi; Naito, Munekazu; Ito, Yasuhiko. Roles of glomerular endothelial hyaluronan in the development of proteinuria. Physiological Reports. 2021;9(17):e15019. 

Katoh, Shojiro; Yoshioka, Hiroshi; Senthilkumar, Rajappa; Preethy, Senthilkumar; Abraham, Samuel J K. Enhanced expression of hyaluronic acid in osteoarthritis-affected knee-cartilage chondrocytes during three-dimensional in vitro culture in a hyaluronic-acid-retaining polymer scaffold. The Knee. 2021;29:365-373. 

Uemura, Akiko; Ogawa, Shingo; Tanaka, Ryou. Intrathoracic retention of insoluble hyaluronic acid and its absorption process in rats. The International Journal Of Artificial Organs. 2020;43(4):283-287. 

Yoshino, Yuta; Ishisaka, Mitsue; Tsuruma, Kazuhiro; Shimazawa, Masamitsu; Yoshida, Hiroyuki; Inoue, Shintaro; Shimoda, Masayuki; Okada, Yasunori; Hara, Hideaki. Distribution and function of hyaluronan binding protein involved in hyaluronan depolymerization (HYBID, KIAA1199) in the mouse central nervous system. Neuroscience. 2017;347:1-10. 

Kakizaki, Ikuko; Miura, Ayako; Mineta, Takashi; Hong, Jinseo; Kato, Yoji. Characterization of Proteoglycan and Hyaluronan in Hot Water Extract from Salmon Cartilage. Journal Of Applied Glycoscience. 64(4):83-90. 

Maeda, H; Fujita, H; Sakura, Y; Miyazaki, K; Goto, M. A competitive enzyme-linked immunosorbent assay-like method for the measurement of urinary hyaluronan. Bioscience, Biotechnology, And Biochemistry. 1999;63(5):892-5. 

Haruna Asano et., al.. A Case of Aseptic Abscess Formation in The Pectoralis Major Muscle 10 Years After Breast Augmentation with Hyaluronic Acid. International Journal of Surgical Wound Care. 5(2):62-66.