Human Collagen type I, ELISA Kit

ACEL

Catalog No.: ACE-EC1-E105

Product Description

This is an ELISA kit that allows for simple and rapid competitive measurement of native collagen. It can be used to quantify both culture supernatant and tissue homogenates.

Features

• Native Collagen Measurement
  This ELISA kit allows for the quantification of collagen in its natural molecular structure. (For amino acid analysis, the molecular structure is broken down and the amount of hydroxyproline is measured.)
• Simple
  The system uses a 96-well microtiter plate, allowing for simultaneous measurement of multiple samples.
  (Up to 80 samples in single replicates, 40 samples in duplicates, and 26 samples in triplicates.)
• Rapid
  Collagen is pre-immobilized on the plate, allowing for rapid quantification (reaction time of 2 hours and 30 minutes).

Human Collagen type I, ELISA Kit FAQs

Can this kit be used to measure changes in collagen levels in cultured cells using Human Collagen Type I ELISA Kit (without pepsin: Cat. No. ACE-EC1-E105 / with pepsin: Cat. No. ACE-EC1-E205)?
Human Collagen Type I ELISA Kit allows for the measurement of collagen both in culture supernatants and as extracellular matrix.

Does the serum concentration in the culture medium affect the measurement when using Human Collagen Type I ELISA Kit (without pepsin: Cat. No. ACE-EC1-E105 / with pepsin: Cat. No. ACE-EC1-E205)?
No. Serum concentration does not influence the measurement based on our internal validation.

What is the range of concentrations that can be measured using Human Collagen Type I ELISA Kit (without pepsin: Cat. No. ACE-EC1-E105 / with pepsin: Cat. No. ACE-EC1-E205)?
The detection limits are 0.02 µg/mL at the lower end and 40 µg/mL at the upper end. Since standards of 0.16 to 10 µg/mL are used in actual measurements, this range is recommended.

Can I use Human Collagen Type I ELISA Kit (without pepsin: Cat. No. ACE-EC1-E105 / with pepsin: Cat. No. ACE-EC1-E205) with non-human samples?
No. Human Collagen Type I ELISA Kit is specific to human collagen and does not cross-react with the species tested (bovine and chick).

What antibody is used in Human Collagen Type I ELISA Kit (without pepsin: Cat. No. ACE-EC1-E105 / with pepsin: Cat. No. ACE-EC1-E205)?
Human Collagen Type I ELISA Kit uses a polyclonal antibody raised against atelocollagen. Because atelocollagen is used as the antigen, the antibody is believed to recognize the triple helix structure.

What is the pH of the "200 mM Tris, 150 mM NaCl solution" used with Human Collagen Type I ELISA Kit (without pepsin: Cat. No. ACE-EC1-E105 / with pepsin: Cat. No. ACE-EC1-E205)?
As an example, the pH is approximately 10.5 when the solution is prepared using Tris and NaCl from FUJIFILM Wako Pure Chemical Corporation. pH adjustment is not required.

What is the measurement wavelength for plate readers when using Human Collagen Type I ELISA Kit (without pepsin: Cat. No. ACE-EC1-E105 / with pepsin: Cat. No. ACE-EC1-E205)?
The measurement wavelength is 450 nm.

Which cell lines have been used to measure collagen in culture supernatants with Human Collagen Type I ELISA Kit (without pepsin: Cat. No. ACE-EC1-E105 / with pepsin: Cat. No. ACE-EC1-E205)?
We have experience measuring collagen in culture supernatants of human dermal fibroblasts and human lung fibroblasts using Human Collagen Type I ELISA Kit.