Pepsin for Human Collagen Type I ELISA Kit

ACEL

Catalog No.: ACE-EC1-E110

When collagen production cell (fibroblast) is cultured in petri-dish, collagen in culture media deposit as ECM. The deposited collagen or tissuecollagen can be eluted by pepsin digestion.

Prepare pepsin solution (Dissolve pepsin powder into 50 mM acetic acid solution to final concentration 0.1 mg/mL).

 

Frequently asked questions

Can this kit be used to measure changes in collagen levels in cultured cells?
This kit allows for measurement of collagen both in culture supernatants and as extracellular matrix.

Does the serum concentration in the culture medium affect the measurement?
Serum concentration does not influence the measurement based on our internal validation.

What is the range of concentrations that can be measured?
The detection limits are 0.02 µg/mL at the lower end and 40 µg/mL at the upper end.
Since standards of 0.16 to 10 µg/mL are used in actual measurements, this range is recommended.

Can this kit be used in non-human samples?
This kit is specific to human collagen, and does not cross-react with samples from the species that were tested (bovine, chick)

What is the antibody used in this kit?
We use a polyclonal antibody that reacts to atelocollagen as an antigen. The antibody is believed to recognize and bind to the triple helix structure of atelocollagen.

What is the pH of the “200 mM Tris, 150 mM NaCl solution” used in the measurement?
No pH adjustment is necessary. For example, the pH of a “200mM Tris, 150mM NaCl solution” prepared using Tris and NaCl from Fujifilm Wako Pure Chemical Industries, Ltd. is approximately 10.5.

What is the measurement wavelength for plate readers?
The measurement wavelength is 450 nm.

Which cell lines have this kit been validated with?

This kit has been validated in human dermal fibroblasts and human lung fibroblasts.

 

References

In Vitro CO-Releasing and antioxidant properties of sulfonamide-based CAI-CORMs in a H2O2-stimulated human achilles tendon-derived cell model
Molecules 2025
 
Silencing of ERRα gene represses cell proliferation and induces apoptosis in human skin fibroblasts.
Molecular Medicine Reports 2024
 
Snail slime extracted by a cruelty free method preserves viability and controls inflammation occurrence: a focus on fibroblasts.
Molecules 2023
 
The open cell form of 3D-printed titanium improves osteconductive properties and adhesion behavior of dental pulp stem cells.
Materials 2021
 
Liposomes embedded with differentiating factors as a new strategy for enhancing DPSC osteogenic commitment.
European Cells & Materials Journal 2021
 
Dual acting carbon monoxide releasing molecules and carbonic anhydrase inhibitors differentially modulate inflammation in human tenocytes.
Biomedicines 2021
 
Bisphosfonate matrix metalloproteinase inhibitors for the treatment of periodontitis: An in vitro study.
International Journal of Molecular Medicine 2018
 
Intestinal myofibroblast TRPC6 channel may contribute to stenotic fibrosis in crohn’s disease.
Inflammatory Bowel Diseases 2015
 
Differences between protein expression and extracellular matrix state on uniaxial stretching for tenogenic differentiation.
Journal of Mechanics in Medicine and Biology 2014
 
Fibroblasts from the growing margin of keloid scars produce higher levels of collagen I and III compared with intralesional and extralesional sites: clinical implications for lesional site-directed therapy.
British Journal of Dermatology 2011