Product Description

The Bacterial EV ELISA Kit captures extracellular vesicles (EVs) on the plate surface by utilizing their negatively charged membranes. By employing an anti-LTA (lipoteichoic acid) antibody for EVs derived from Gram-positive bacteria, this kit enables the relative quantification of microbial EVs without the need for expensive instrumentation.

Features

• Relative quantification of microbial-derived EVs can be performed using the same procedure as a conventional ELISA.
• Reference materials included for immediate use.
• High sensitivity enables analysis with small sample volumes.
   Detection limit: 4.0 × 10^5 particles/mL

If the sample contains a high concentration of salt, EVs may not be captured on the plate.
Before use, perform buffer exchange or dilute the sample with phosphate buffer or a similar buffer so that the salt concentration is 150 mM or less.

Product Data

Examples of standard curve for Gram-positive Bacterial EV ELISA Kit
Fig. Examples of standard curve for Gram-positive Bacterial EV ELISA Kit

Product  Specifications

Reactivity Gram-positive bacterial EV
Assay Principle quantitative
Detection limit 4.0 × 10^5 particles/mL
Measurement Type Sandwich
Detection Wavelength 450 nm
Sample Volume 100ul



Documents & Links for Gram-positive bacterial EV ELISA Kit
Datasheet Gram-positive bacterial EV ELISA Kit Datasheet

Documents & Links for Gram-positive bacterial EV ELISA Kit
Datasheet Gram-positive bacterial EV ELISA Kit Datasheet