Gram-negative bacterial EV ELISA Kit

Cosmo Bio

Catalog No.: CSR-EVEL02

Product Description

The Bacterial EV ELISA Kit captures extracellular vesicles (EVs) on the plate surface by utilizing their negatively charged membranes. By employing an anti-LPS (lipopolysaccharide) antibody for EVs derived from Gram-negative bacteria, this kit enables the relative quantification of microbial EVs without the need for expensive instrumentation.

Features

• Relative quantification of microbial-derived EVs can be performed using the same procedure as a conventional ELISA.
• Reference materials included for immediate use.
• High sensitivity enables analysis with small sample volumes.
   Detection limit: 8.0 × 10^6 particles/mL

If the sample contains a high concentration of salt, EVs may not be captured on the plate.
Before use, perform buffer exchange or dilute the sample with phosphate buffer or a similar buffer so that the salt concentration is 150 mM or less.

Product Data

Fig. Example of standard curve for Gram-negative Bacterial EV ELISA Kit
For the E. coli EV standard, measurements were performed in the presence of a 370-fold excess of human milk exosomes (1.9 × 10^11 human milk exosomes added to 5.12 × 10^8 E. coli exosomes). Accurate quantification of E. coli exosomes was maintained even in the presence of a large excess of non-target EVs. 

Product Specifications

Reactivity	Gram-negavite bacterial EV
Assay Principle	quantitative
Detection limit	8.0 × 10^6 particles
Measurement Type	Sandwich
Detection Wavelength	450 nm
Sample Volume	100 ul