DnaK Mix

Catalog No:
GFK-PF003-0.5-EX
$158.00

DnaK Mix is a newly developed supplement for the PUREfrex® series reconstituted coupled transcription/translation systems, designed to promote proper folding and improve solubility for proteins that require molecular chaperones for proper folding.

It comprises an optimized ratio of highly purified E.coli-derived DnaK, DnaJ and GrpE.

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DnaK Mix is a newly developed supplement for the PUREfrex® series reconstituted coupled transcription/translation systems, designed to promote proper folding and improve solubility for proteins that require molecular chaperones for proper folding. It comprises an optimized ratio of highly purified E.coli-derived DnaK, DnaJ and GrpE.

DnaK Mix is a newly developed supplement for the PUREfrex® series, designed to assist proper folding and improve solubility of proteins that require molecular chaperones for proper protein folding. DnaK Mix comprises an optimized ratio of highly purified E.coli-derived DnaK, DnaJ and GrpE. ATPase activity of DnaK (Hsp70) is stimulated by co-chaperones DnaJ and GrpE. DnaJ promotes the ATPase activity of DnaK and can bind to hydrophobic regions of synthesized proteins. GrpE stimulates ADP/ATP exchange rate of DnaK.

Chaperone Supplement

DnaK Mix comprises highly purified and ratio-optimized E. coli DnaK, DnaJ and GrpE. DnaK, known as Hsp70, has ATPase activity and is stimulated by co-chaperones DnaJ and GrpE. DnaJ facilitates DnaK ATPase activity and binds to hydrophobic regions of synthesized proteins. GrpE stimulates ADP/ATP exchange of DnaK. DnaK Mix works well with PUREfrex®series and DsbC Set.

Reagent Qty Description Storage
DnaK Mix 25 ul  100µM DnaK, 20µM DnaJ and 20µM GrpE from E.coli in 30% glycerol buffer (No tags)  -80 C
 Dilution Buffer 500 ul  30% glycerol buffer   -20 C
       

Chaperone Supplement

GroE Mix comprises highly purified and ratio-optimized E. coli GroEL (known as Hsp60) and GroES (works in conjunction with GroEL). 

  

Reagent Qty Description Storage
 GroE Mix 12.5 ul   20µM GroEL, 14-mer form, and 40µM GroES, 7-mer form, from E.coli in 30% glycerol buffer (No tags)  -80 C
 Dilution Buffer 500 ul  30% glycerol buffer   -20 C