PUREfrex 2.0

Cosmo Bio LTD

Catalog No: GFK-PF201-0.25-EX



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PUREfrex^® 2.0 reconstituted coupled transcription/translation system is a new and improved version of PUREfrex^® 1.0 that debuted in 2011. By improving the purification process for components of PUREfrex^® 2.0RNase, β-galactosidase and LPS contamination has been reduced, the latter to around 0.1EU/µL of reaction mixture. This, together with a newly optimized composition has boosted protein yield 2-10 times compared to PUREfrex^® 1.0. Further, all proteinaceous components of PUREfrex^® 2.0 are free of fusion tags, allowing users the freedom to incorporate any chosen tag for protein purification/detection.

For proteins whose functional conformation requires the assistance of molecular chaperones or the creation of disulfide bonds, we offer supplemental reagents that work in conjunction with PUREfrex^® 2.0.

Supplements – Molecular Chaperones

These are supplements for PUREfrex^® series reconstituted coupled transcription/translation systems to promote production and solubility of aggregation-prone proteins.


• DnaK Mix (#PF003-0.5-EX)

• GroE Mix (#PF004-0.5-EX)


Supplements – Forming disulfide bonds

These are supplements for PUREfrex^® series reconstituted coupled transcription/translation systems to promote synthesis of functional proteins containing disulfide bonds.


• DsbC Set (#PF005-0.5-EX)

• PDI Set (#PF006-0.5-EX)






Citations for PUREfrex 2.0 – 17 Found

Bordeau et al. 2014. Curli synthesis and biofilm formation in enteric bacteria are controlled by a dynamic small RNA module made up of a pseudoknot assisted by an RNA chaperone. Nucleic Acids Res. 42(7):4682-96.   , Journal

Bordeau et al. 2014. Curli synthesis and biofilm formation in enteric bacteria are controlled by a dynamic small RNA module made up of a pseudoknot assisted by an RNA chaperone. Nucleic Acids Res. 42(7):4682-96.   , Journal

Vázquez-Laslop et al. 2011. Role of antibiotic ligand in nascent peptide-dependent ribosome stalling. PNAs. 108(26):10496-501.   , Journal

Ullman et al. 2011. In vitro methods for peptide display and their applications. Brief Funct Genomics. 10(3):125-34.   , Journal

Narayan et al. 2011. A multiprotein binding interface in an intrinsically disordered region of the tumor suppressor protein interferon regulatory factor-1. J Biol Chem. 286(16):14291-303.   , Journal

Handa et al. 2011. YaeJ is a novel ribosome-associated protein in Escherichia coli that can hydrolyze peptidyl-tRNA on stalled ribosomes. Nucleic Acids Res. 39(5):1739-48.   , Journal

Yamaguchi et al. 2010. Cortisol is involved in temperature-dependent sex determination in the Japanese flounder. Endocrinology. 151(8):3900-8.   , Journal

Asahara et al. 2010. In vitro genetic reconstruction of bacterial transcription initiation by coupled synthesis and detection of RNA polymerase holoenzyme. Nucleic Acids Res. 38(13):e141.   , Journal

Uchida et al. 2009. Salmonella enterica serotype Typhimurium DT104 ArtA-dependent modification of pertussis toxin-sensitive G proteins in the presence of [32P]NAD. Microbiology. 155(11):3710-8.   , Journal

Yamamoto et al. 2009. Roles of Tom70 in import of presequence-containing mitochondrial proteins. J Biol Chem. 284(46):31635-46.   , Journal

Terashima et al. 2008. Cell-free synthesis of the torque-generating membrane proteins, PomA and PomB, of the Na+-driven flagellar motor in Vibrio alginolyticus. J Biochem. 144(5):635-42.   , Journal

Maki et al. 2008. RNA, but not protein partners, is directly responsible for translational silencing by a bacterial Hfq-binding small RNA. PNAs. 105(30):10332-7.   , Journal

Yamano et al. 2008. Tom20 and Tom22 share the common signal recognition pathway in mitochondrial protein import. J Biol Chem. 283(7):3799-807.   , Journal

Sharma et al. 2007. A small RNA regulates multiple ABC transporter mRNAs by targeting C/A-rich elements inside and upstream of ribosome-binding sites. Genes Dev. 21(21):2804-17.   , Journal

Shimizu et al. 2006. SmpB triggers GTP hydrolysis of elongation factor Tu on ribosomes by compensating for the lack of codon-anticodon interaction during trans-translation initiation. J Biol Chem. 281(23):15987-96.   , Journal

Fukushima et al. 2005. Functional role played by the glycosylphosphatidylinositol anchor glycan of CD48 in interleukin-18-induced interferon-gamma production. J Biol Chem. 280(18):18056-62.   , Journal

Yano et al. 2005. Involvement of Hu and heterogeneous nuclear ribonucleoprotein K in neuronal differentiation through p21 mRNA post-transcriptional regulation. J Biol Chem. 280(13):12690-9.   , Journal