Collagen Quantitation Kit

Cosmo Bio

Catalog No.: CSR-COL-001

Collagen is the one of the main components of extracellular matrix, and accounts for about 30% of the whole human protein. Recent
studies, the production of collagen are reduced in aged skin, the degradation and the accumulation ofcollagen found in a particular　disease, such as has been shown. Therefore, it has become important in accurately quantifying technology health maintenance and　disease diagnosis collagen.　Collagen contains approximately 30 % Glycine (Gly) of whole amino acid residues in each molecule, and constitutes a repeated amino acid sequence, -Gly-X-Y- in which Pro or Hyp frequently appears at the X or Y position. The proposed assay method takes an advantage of this unique sequence of collagen aswell as the remarkable specifi city of bacterial collagenase that cleaves all collagen types at the position of　N-terminal side of the Gly residue. Therefore, abundant N-terminal Gly-containing peptides such as Gly-Pro-Hyp, Gly-Pro-Ala, Gly-Pro-Pro, and Gly-Pro-Z (Z is other amino acid or oligopeptide) are produced from one molecule of each collagen, and greatly amplifi ed fl uorescence signals from these products are obtained by the reaction with 3,4-Dihydroxyphenylacetic acid (3,4-DHPAA).　This kit is a highly specific and sensitive method for the assay of whole collagen in biological samples using a fluorogenic reagent, 3,4-Dihydroxyphenylacetic acid (3,4-DHPAA). The 3, 4-DHPAA reagent can selectively detect N-terminal Gly-containing peptides.

Features

This kit can quickly measure the collagen in the food and cosmetics. Compared to the existing hydroxyproline method, there is no need of hydrochloric acid hydrolysis, it can be determined in a safe and simple procedure with a small amount of sample. This kit is suitable for quality control and product development during the manufacturing process.
● Simple procedure: takes only 2 - 3 hours
● Fast and convenient

Citations for Collagen Quantitation Kit – 9 Found

Machihara, Kayo; Oki, Shoma; Maejima, Yuka; Kageyama, Sou; Onda, Ayumu; Koseki, Yurino; Imai, Yasuyuki; Namba, Takushi. Restoration of mitochondrial function by Spirulina polysaccharide via upregulated SOD2 in aging fibroblasts. Iscience. 2023;26(7):107113. 

Katsuura, Misako; Homma, Jun; Higashi, Yuhei; Sekine, Hidekazu; Shimizu, Tatsuya. Author Correction: Densely vascularized thick 3D tissue shows enhanced protein secretion constructed with intermittent positive pressure. Communications Biology. 2025;8(1):388. 

Machihara, Kayo; Kageyama, Sou; Oki, Shoma; Makino, Hiroki; Sasaki, Masamichi; Iwahashi, Hiroyasu; Namba, Takushi. Lotus germ extract rejuvenates aging fibroblasts via restoration of disrupted proteostasis by the induction of autophagy. Aging. 2022;14(19):7662-7691. 

Hashimoto, Kei; Yamashita, Kimiko; Enoyoshi, Kanako; Dahan, Xavier; Takeuchi, Tatsu; Kori, Hiroshi; Gotoh, Mari. The effects of coating culture dishes with collagen on fibroblast cell shape and swirling pattern formation. Journal Of Biological Physics. 2020;46(4):351-369. 

Iimaa, Tuyajargal; Ikegami, Yasuhiro; Bual, Ronald; Shirakigawa, Nana; Ijima, Hiroyuki. Analysis of Sulfated Glycosaminoglycans in ECM Scaffolds for Tissue Engineering Applications: Modified Alcian Blue Method Development and Validation. Journal Of Functional Biomaterials. 2019;10(2) 

Bual, Ronald P; Ijima, Hiroyuki. Intact extracellular matrix component promotes maintenance of liver-specific functions and larger aggregates formation of primary rat hepatocytes. Regenerative Therapy. 2019;11:258-268. 

Machihara, Kayo; Kageyama, Sou; Oki, Shoma; Makino, Hiroki; Sasaki, Masamichi; Iwahashi, Hiroyasu; Namba, Takushi. Lotus germ extract rejuvenates aging fibroblasts via restoration of disrupted proteostasis by the induction of autophagy. Aging. 2022;14(19):7662-7691. 

Maejima, Yuka; Morioka, Yuki; Sato, Yusei; Hiraoka, Masanori; Onda, Ayumu; Namba, Takushi. Ulvan-Na, an Ulvan Subjected to Na(+) Cation Exchange, Improves Intestinal Barrier Function in Age-Related Leaky Gut. Marine Drugs. 2025;23(10) 

Tomohiro Itoh et., al. . Role of Royal Jelly Treated Adipose-Derived Stem Cell-Extracellular Vesicles on Fibroblast Proliferation, Migration, and Collagen Production. Dermatologic Therapy. 2023;(1):21.