Anti LGALS3 mAb (Clone 4E1)

CUSABIO

Catalog No.:
CSB-RA970289A0HU-50
Western Blot<br /> Positive WB detected in: MCF-7 whole cell lysate, Hela whole cell lysate, NIH/3T3 whole cell lysate, Colo320 whole cell lysate, HT29 whole cell lysate<br /> All lanes: Galectin 3 antibody at 1:2000<br /> Secondary<br /> Goat polyclonal to rabbit IgG at 1/50000 dilution<br /> Predicted band size: 27 kDa<br /> Observed band size: 31 kDa<br />
IHC image of CSB-RA970289A0HU diluted at 1:100 and staining in paraffin-embedded human ovarian cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
Immunofluorescence staining of MCF7 Cells with CSB-RA970289A0HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% TritonX-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4℃. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L).
Immunoprecipitating LGALS3 in Hela whole cell lysate<br /> Lane 1: Rabbit control IgG instead of CSB-RA970289A0HU in Hela whole cell lysate. For western blotting,a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)<br /> Lane 2: CSB-RA970289A0HU(2µg)+ Hela whole cell lysate(500µg)<br /> Lane 3: Hela whole cell lysate (10µg)<br />
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Galactose-specific lectin which binds IgE. May mediate with the alpha-3, beta-1 integrin the stimulation by CSPG4 of endothelial cells migration. Together with DMBT1, required for terminal differentiation of columnar epithelial cells during early embryogenesis (By similarity). In the nucleus: acts as a pre-mRNA splicing factor. Involved in acute inflammatory responses including neutrophil activation and adhesion, chemoattraction of monocytes macrophages, opsonization of apoptotic neutrophils, and activation of mast cells.

Product Specifications
Application WB, IHC, IF, IP, ELISA
Reactivity Human
Clonality Monoclonal (Clone No.: 40)

Documents & Links for Anti LGALS3 mAb (Clone 4E1)
Datasheet Anti LGALS3 mAb (Clone 4E1) Datasheet

Documents & Links for Anti LGALS3 mAb (Clone 4E1)
Datasheet Anti LGALS3 mAb (Clone 4E1) Datasheet
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