Anti-IL-18 (Clone 74) (Mouse) mAb (Clone: 74)

MBL Life Sciences

Catalog No.: MBL-D047-3

BACKGROUND

Monoclonal antibody of 100 µl targeting IL-18 for IPP.

Interleukin 18 (IL-18) is a 18 kDa cytokine which identified as a costimulatory factor for production of interferon-γ (IFN-γ) in response to toxic shock and shares functional similarities with IL-12. IL-18 is synthesized as a precursor 24 kDa molecule without a signal peptide and must be cleaved to produce an active molecule. IL-1 converting enzyme (ICE, Caspase-1) cleaves pro-IL-18 at aspartic acid in the P1 position, producing the mature, bioactive peptide that is readily released from the cells. It is reported that IL-18 is produced from Kupffer cells, activated macrophages, keratinocytes, intestinal epithelial cells, osteoblasts,
adrenal cortex cells and murine diencephalon. IFN-γ is produced by activated T or NK cells and plays critical roles in the defense against microbiral pathogens. IFN-γ activates macrophages, enhances NK activity and B cell
maturation, proliferation and Ig secretion, induces MHC class I and II antigens, and inhibits osteoclast activation.
IL-18 acts on T helper type-1 (Th1) T cells and in combination with IL-12 strongly induces them to produce IFN-γ. Pleiotropic effects of IL-18 has also been reported, such as, enhancement production of IFN-γ and GM-CSF
in peripheral blood mononuclear cells, production of Th1 cytokines, IL-2, GM-CSF and IFN-γ in T cells,
enhancement of Fas ligand expression by Th1 cells.

SOURCE

This antibody was purified from hybridoma (clone 74) supernatant using protein G agarose. This hybridoma was established by fusion of mouse myeloma cell Y3Ag 1.2.3 with SD rat splenocyte immunized with recombinant mouse IL-18

Product Specifications

• Application: IP
• Reactivity: Mouse
• Clonality: Monoclonal IgG2a
• Host: Rat
• Immunogen: Recombinant mouse IL-18
• Gene ID - Human:

  3606

• Gene ID - Mouse:

  16173

• Formulation: 100 µg IgG in 100 µl volume of PBS containing 50% glycerol, pH 7.2. No preservative is contained.
• Storage: -20C

Citations for Anti-IL-18 (Clone 74) (Mouse) mAb (Clone: 74) – 10 Found

Luo, Y; Yamada, H; Chen, X; Ryan, A A; Evanoff, D P; Triccas, J A; O'Donnell, M A. Recombinant Mycobacterium bovis bacillus Calmette-Guérin (BCG) expressing mouse IL-18 augments Th1 immunity and macrophage cytotoxicity. Clinical And Experimental Immunology. 2004;137(1):24-34. 
- Comments: Used in: WB

Abu Elhija, Mahmoud; Lunenfeld, Eitan; Eldar-Geva, Talia; Huleihel, Mahmoud. Over-expression of IL-18, ICE and IL-18 R in testicular tissue from sexually immature as compared to mature mice. European Cytokine Network. 2008;19(1):15-24. 
- Comments: Used in: ELISA

Abu Elhija, Mahmoud; Lunenfeld, Eitan; Huleihel, Mahmoud. LPS increases the expression levels of IL-18, ICE and IL-18 R in mouse testes. American Journal Of Reproductive Immunology (New York, N.y. : 1989). 2008;60(4):361-71. 
- Comments: Used in: ELISA

Murphy, Andrew J; Kraakman, Michael J; Kammoun, Helene L; Dragoljevic, Dragana; Lee, Man K S; Lawlor, Kate E; Wentworth, John M; Vasanthakumar, Ajithkumar; Gerlic, Motti; Whitehead, Lachlan W; DiRago, Ladina; Cengia, Louise; Lane, Rachael M; Metcalf, Donald; Vince, James E; Harrison, Leonard C; Kallies, Axel; Kile, Benjamin T; Croker, Ben A; Febbraio, Mark A; Masters, Seth L. IL-18 Production from the NLRP1 Inflammasome Prevents Obesity and Metabolic Syndrome. Cell Metabolism. 2016;23(1):155-64. 
- Comments: Used in: WB

Wang, Xiaowei; Shaw, Dana K; Sakhon, Olivia S; Snyder, Greg A; Sundberg, Eric J; Santambrogio, Laura; Sutterwala, Fayyaz S; Dumler, J Stephen; Shirey, Kari Ann; Perkins, Darren J; Richard, Katharina; Chagas, Andrezza C; Calvo, Eric; Kopecký, Jan; Kotsyfakis, Michail; Pedra, Joao H F. The Tick Protein Sialostatin L2 Binds to Annexin A2 and Inhibits NLRC4-Mediated Inflammasome Activation. Infection And Immunity. 2016;84(6):1796-1805. 
- Comments: Used in: ELISA

Wang, Xiaowei; Shaw, Dana K; Hammond, Holly L; Sutterwala, Fayyaz S; Rayamajhi, Manira; Shirey, Kari Ann; Perkins, Darren J; Bonventre, Joseph V; Velayutham, Thangam S; Evans, Sean M; Rodino, Kyle G; VieBrock, Lauren; Scanlon, Karen M; Carbonetti, Nicholas H; Carlyon, Jason A; Miao, Edward A; McBride, Jere W; Kotsyfakis, Michail; Pedra, Joao H F. The Prostaglandin E2-EP3 Receptor Axis Regulates Anaplasma phagocytophilum-Mediated NLRC4 Inflammasome Activation. Plos Pathogens. 2016;12(8):e1005803. 
- Comments: Used in: ELISA

Weindel, Chi G; Richey, Lauren J; Mehta, Abhiruchi J; Shah, Mansi; Huber, Brigitte T. Autophagy in Dendritic Cells and B Cells Is Critical for the Inflammatory State of TLR7-Mediated Autoimmunity. Journal Of Immunology (Baltimore, Md. : 1950). 2017;198(3):1081-1092. 
- Comments: Used in: ELISA

Lasseaux, Corentin; Fourmaux, Marie-Pierre; Chamaillard, Mathias; Poulin, Lionel Franz. Type I interferons drive inflammasome-independent emergency monocytopoiesis during endotoxemia. Scientific Reports. 2017;7(1):16935. 
- Comments: Used in: ELISA

Nichols, Randilea D; von Moltke, Jakob; Vance, Russell E. NAIP/NLRC4 inflammasome activation in MRP8(+) cells is sufficient to cause systemic inflammatory disease. Nature Communications. 2017;8(1):2209. 
- Comments: Used in: ELISA

Flood, Brian; Manils, Joan; Nulty, Ciara; Flis, Ewelina; Kenealy, Sinead; Barber, Gillian; Fay, Joanna; Mills, Kingston H G; Kay, Elaine W; Creagh, Emma M. Caspase-11 regulates the tumour suppressor function of STAT1 in a murine model of colitis-associated carcinogenesis. Oncogene. 2019;38(14):2658-2674. 
- Comments: Used in: ELISA