H-2Db LCMV gp33 Tetramer-KAVYNFATC-BV421

MBL Life Sciences

SKU: MBL-TS-5002-4C

Anti-mouse CD8 clone choice is critical when mouse MHC I tetramers, as some clones, including 53-6.7, lead to high background staining of all CD8+ T cells, rather than the antigen-specific ones. For best results, clone KT15, available from MBL International in FITC (K0227-4)is recommended. Additional information can be found in the datasheet that accompanies mouse class I tetramers, as well as the following reference containing a protocol for performing a cross titration series for optimization: Current Protocols in Immunology, MHC-Peptide tetramers to Visualize Antigen-Specific T Cells, Section 17.3.23-24, Supplement 58

Product Specifications

• Application: FC
• Reactivity: Mouse
• Conjugation: BV421
• Peptide Sequence: KAVYNFATC
• Class: Class I
• Sequence Location: 33-41
• MHC Allele: H-2D^b

Citations for H-2Db LCMV gp33 Tetramer-KAVYNFATC-BV421 – 1 Found

Liu, Qiao; Zhang, Liang; Shu, Zhou; Yu, Tingting; Zhou, Lina; Song, Wenxia; Zhao, Xiaodong. WASp Is Essential for Effector-to-Memory conversion and for Maintenance of CD8(+)T Cell Memory. Frontiers In Immunology. 10:2262. 
- Comments: Used in: FCM