The T7 tag is an 11–amino acid epitope (sequence: MASMTGGQQMG) derived from the major capsid protein (gene 10) of bacteriophage T7. It is widely used as a fusion tag for recombinant proteins, enabling efficient detection and characterization in diverse expression systems.
Epitope tagging with T7 allows researchers to:
-Detect and localize recombinant proteins in various cell types.
-Study protein topology and protein–protein interactions.
-Identify binding partners and associated protein complexes.
-Characterize low-abundance or poorly immunogenic proteins where specific antibodies are not available.
This monoclonal antibody specifically recognizes the T7 epitope and is optimized for Western blotting, providing sensitive and reliable detection of T7-tagged proteins in research applications.
Product Application
WB: 1:2000 – 1:5000
Specificity: Recognizes T7 tag fusion proteins. Format: Liquid
Product Specifications
Application
WB
Clonality
Monoclonal IgG
Host
Mouse
Conjugation
KLH
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence of T7-tag. The exact sequence is proprietary
