S100A9 Assay Kit (rat)

YAMASA

Catalog No.: YMS-80126-EX

Product Description

Assay principle of this kit is the solid phase enzyme-linked immunosorbent assay (ELISA) using two monoclonal antibodies against rat S100A9 and rat S100A9 as a standard material. It is possible to measure the concentration of rat S100A9 in serum by this kit.

Species of Origin: Rat
Cross-reactivity: Rat

Purpose of Use:
Calprotectin is a complex of two proteins, S100A8 and S100A9, and is known as a biomarker for inflammatory bowel disease (IBD). At Yamasa Corporation, we have developed an ELISA for measuring rat S100A9 using a monoclonal antibody against S100A9, which is one of the components of rat calprotectin.
Using this ELISA, we measured S100A9 in the feces of rats with ulcerative colitis (UC) induced by dextran sulfate sodium (DSS). The results showed an increase in S100A9 concentration from the early stages of inflammation, suggesting that it serves as a good indicator for the UC model.
This ELISA was then made into a commercial product as the Rat S100A9 Assay Kit.

Kit Components:

1. Antibody-coated plate
2. S100A9 Standard Solution 1 (3.75 ng/mL)
3. S100A9 Standard Solution 2 (15 ng/mL)
4. S100A9 Standard Solution 3 (60 ng/mL)
5. S100A9 Standard Solution 4 (240 ng/mL)
6. Dilution Buffer (3× concentrate)
7. Wash Solution (5× concentrate)
8. Enzyme-labeled antibody
9. Color Reagent A
10. Color Reagent B
11. Stop Solution