Rat neuronal stem cell line 1464R

Cosmo Bio

Catalog No.: PMC-RNSCL-C

Neural stem cell line 1464R is a spontaneously immortalized cell line obtained from brain stem cultures of adult male Fischer 344 rats. It can be cultured for a long period of time and maintained without morphological changes even after repeated passaging. In the presence of retinoic acid, 1464R cells stop dividing and differentiate mainly into tubulin beta-III (TuJ1)-positive neurons. They also differentiate into GFAP-positive astrocytes and O4-positive oligodendrocytes as glial cell lineages. This product was established by Professor Kazuhiko Watabe of the Laboratory of Molecular Neurobiology, Kyorin University School of Health Sciences, and is sold under license from the Tokyo Metropolitan Institute of Medical Science.

Citations for Rat neuronal stem cell line 1464R – 6 Found

Watabe, Kazuhiko; Akiyama, Keiko; Kawakami, Emiko; Ishii, Tomohiro; Endo, Kentaro; Yanagisawa, Hiroko; Sango, Kazunori; Tsukamoto, Masami. Adenoviral expression of TDP-43 and FUS genes and shRNAs for protein degradation pathways in rodent motoneurons in vitro and in vivo. Neuropathology : Official Journal Of The Japanese Society Of Neuropathology. 2014;34(1):83-98. 

Ishii, Tomohiro; Kawakami, Emiko; Endo, Kentaro; Misawa, Hidemi; Watabe, Kazuhiko. Myelinating cocultures of rodent stem cell line-derived neurons and immortalized Schwann cells. Neuropathology : Official Journal Of The Japanese Society Of Neuropathology. 2017;37(5):475-481. 

Ishii, Tomohiro; Kawakami, Emiko; Endo, Kentaro; Misawa, Hidemi; Watabe, Kazuhiko. Formation and spreading of TDP-43 aggregates in cultured neuronal and glial cells demonstrated by time-lapse imaging. Plos One. 12(6):e0179375. 

Watabe, Kazuhiko; Kato, Yoichiro; Sakuma, Miho; Murata, Makiko; Niida-Kawaguchi, Motoko; Takemura, Taro; Hanagata, Nobutaka; Tada, Mari; Kakita, Akiyoshi; Shibata, Noriyuki. Praja1 RING-finger E3 ubiquitin ligase suppresses neuronal cytoplasmic TDP-43 aggregate formation. Neuropathology : Official Journal Of The Japanese Society Of Neuropathology. 2020;40(6):570-586. 

Watabe, Kazuhiko; Niida-Kawaguchi, Motoko; Tada, Mari; Kato, Yoichiro; Murata, Makiko; Tanji, Kunikazu; Wakabayashi, Koichi; Yamada, Mitsunori; Kakita, Akiyoshi; Shibata, Noriyuki. Praja1 RING-finger E3 ubiquitin ligase is a common suppressor of neurodegenerative disease-associated protein aggregation. Neuropathology : Official Journal Of The Japanese Society Of Neuropathology. 2022;42(6):488-504. 

Soejima-Kusunoki, Aki; Okada, Kinya; Saito, Ryuta; Watabe, Kazuhiko. The Protective Effect of Edaravone on TDP-43 Plus Oxidative Stress-Induced Neurotoxicity in Neuronal Cells: Analysis of Its Neuroprotective Mechanisms Using RNA Sequencing. Pharmaceuticals (Basel, Switzerland). 2022;15(7) 

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Direct Shipment and Fees Authorization
This item must ship directly from our Japan office due to permit requirements, temperature sensitivity, or other shipping considerations. Access the direct shipment authorization form in the Documents section above. Please download and fill in the document as requested. Submit the completed document with your order to orders@cosmobiousa.com.

Rat neuronal stem cell line 1464R FAQs

It is stated that Rat Neuronal Stem Cell Line 1464R (Cat. No. PMC-RNSCL-C) can be cultured for an extended period. How long can this cell line be maintained in culture? Also, how many times can I passage Rat Neuronal Stem Cell Line 1464R?
According to the professor who developed Rat Neuronal Stem Cell Line 1464R, the cell line can be passaged approximately 50 to 70 times.
The number of passages varies from lot to lot, so please contact us for details.

Can I control the differentiation ratio of Rat Neuronal Stem Cell Line 1464R (Cat. No. PMC-RNSCL-C) to obtain specific proportions of neurons, astrocytes, and oligodendrocytes?
No. You cannot control the differentiation ratio because Rat Neuronal Stem Cell Line 1464R differentiates without the use of forced differentiation agents. The final culture typically consists of approximately 70% neurons, 20% astrocytes, and 10% oligodendrocytes.