QuickSwitch™ Quant H-2 Kb Tetramer Kit-BV421

MBL Life Sciences

SKU: MBL-TS-7400-4R

Anti-mouse CD8 clone choice is critical when mouse MHC I tetramers, as some clones, including 53-6.7, lead to high background staining of all CD8+ T cells, rather than the antigen-specific ones. For best results, clone KT15, available from MBL International in FITC (K0227-4)is recommended. Additional information can be found in the datasheet that accompanies mouse class I tetramers, as well as the following reference containing a protocol for performing a cross titration series for optimization: Current Protocols in Immunology, MHC-Peptide tetramers to Visualize Antigen-Specific T Cells, Section 17.3.23-24, Supplement 143. Please use this link for access to the QuickSwitch™ Peptide Exchange Calculator to check your binding affinity.

Product Specifications

• Application: FC
• Reactivity: Mouse
• Conjugation: BV421
• Peptide Sequence: Custom
• Class: Class I
• MHC Allele: H-2K^b