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A reagent suitable for exfoliation and dispersion of human iPS / ES cells. Pronase is a cell dissociation enzyme.
Contains no animal-derived ingredients.
A high engraftment rate can be obtained by using it together with "CP-5E".
Storage method: -15 ° C or less
Validity period: 24 months
References:
(1) Masao Nomoto, et al .: Research on actinomycete proteases (6th report). Report from RIKEN. 1959, 35(2), p.154-166.
(2) Imaizumi, Keitaro. et al, : A simple and highly effective method for slow-freezing human pluripotent stem cells using dimethyl sulfoxide, hydroxyethyl starch and ethylene glycol. PLoS One. 2014 Feb 12;9(2):e88696.
(3) Imaizumi, Keitaro. et al, : A simple and efficient method of slow freezing for human embryonic stem cells and induced pluripotent stem cells. ES Cells: Methods and Protocols-2nd Edition, Springer Protocols, 2015 in press.