PPIs Detection Reagent : Fluoppi [ Mcl1-BAK ] (pAsh/Mcl1, phAG/BAK cassettes)

MBL Life Sciences

Catalog No.: MBL-AM-P0006

Product Description

Fluoppi is a technology providing an easy way to visualize protein-protein interactions (PPIs) with a high signal to noise ratio. It employs an oligomeric assembly helper tag (Ash-tag) and a tetrameric fluorescent protein tag (FP-tag) to create detectable fluorescent puncta when there are interactions between two proteins fused to the tags. Genetic fusion of protein X with FP-tag, and Y with Ash-tag creates a tetrameric fluorescent fusion protein X-FP and an oligomeric fusion protein Y-Ash respectively. Because each fusion protein has multiple Xs or Ys, the interaction between X and Y causes phase-separated droplets where the fluorescence by X-FP is concentrated and detectable as fluorescent puncta.