Periodic Acid-Schiff (PAS)-Harris Hematoxylin

BQCkit

Catalog No: BQC-KH07012-125

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Purpose of Use:
Periodic Acid Schiff staining technique is used to demonstrate simple polysaccharides, neutral muco-polisaccharides, mucoproteins, and glicolipids. In this kit, Harris hematoxylin is used to stain cell nuclei.

Use and sample preparation:
Designed for research use only, it is not approved for human or animal use, or clinical diagnosis.

Components:

• Schiff Reagent
• Periodic acid 0,5%
• Sulfurose Acid Reagent
• Harris Hematoxylin

Short procedure:

1. Deparaffinize and hydrate
2. Add periodic acid 0,5%
3. Rinse with distilled water
4. Add Schiff Reagent
5. Rinse with 3 baths of sulfurose acid
6. Rinse with tap water
7. Place in hematoxylin
8. Dehydrate, clear and coverslip

Staining characteristics:
Nuclei can be visualized in violet, simple polysaccharides, neutral muco-polisaccharides, glicolipids and basement membrane in red/purple.