Myc-Tag Monoclonal Antibody

ORF Biologics

Catalog No.: ORF.MYCMAB-50

Product Description

The Myc tag is a short peptide sequence derived from the human c-Myc proto-oncogene product, a nuclear phosphoprotein involved in regulating cell cycle progression, apoptosis, and cellular transformation. In its native form, c-Myc functions by forming a heterodimer with the transcription factor MAX. This complex binds to the E-box DNA consensus sequence, controlling the transcription of specific target genes. The Myc gene is frequently amplified in various human cancers, and chromosomal translocations involving this gene are linked to conditions such as Burkitt lymphoma and multiple myeloma. Notably, c-Myc translation can initiate from both an upstream non-AUG (CUG) codon and a downstream AUG start site, producing two isoforms with distinct N-terminal regions. This monoclonal antibody specifically recognizes the Myc epitope, enabling reliable detection, localization, and purification of Myc-tagged recombinant proteins. It is ideal for applications including Western blotting, immunofluorescence, immunoprecipitation, and ELISA, offering high sensitivity and specificity for both native and denatured protein forms.

Product Application

WB: 1:2000 – 1:5000 IF/IC: 1:200 – 1:500 IP: 1:100 – 1:200

Specificity: Recognizes Myc-tag fusion proteins.
Format: Liquid

Product Specifications

• Application: WB, IF, IC, IP
• Clonality: Monoclonal IgG
• Host: Mouse
• Conjugation: KLH
• Immunogen: KLH-conjugated synthetic peptide encompassing a sequence of Myc-tag. The exact sequence is proprietary